...among the first batch of antibodies we produced at Addgene and, as you might suspect, it has been one of our...always best to have options.      Fig. 2: Addgene’s western blot for mEGFP-HRas (from Plasmid 18662... [N86/8R] page for more details. Image credit: Addgene.   Find anti-GFP [N86/8R] here!   Andrews NP...

... Overview of SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid...to find all available sequence information from Addgene depositors and our own sequencing results: On this...suggesting these changes, and the SnapGene and Addgene software development teams for working tirelessly...comments section below and keep checking in at blog.addgene.org for additional enhancements that will further...

... Labeling in Flow Cytometry Resource on addgene.org Addgene's Antibody Guide rAb Affinity Purification...Harvard Medical School.   More resources on the Addgene blog Antibodies 101: Flow Cytometry Conventional...Purification Protocol Video rAbs in the Addgene repository...

... Cytometry Resources on Addgene.org Addgene's Antibody Guide Browse Addgene's Antibody Collection     ...Harvard Medical School.   More resources on the Addgene blog Introduction to Gating in Flow Cytometry Antibodies...

...your foot in the door Resources on Addgene.org Check out how Addgene uses videos for science communication... science communication, including the teams at Addgene, ComSciCon, and many more great organizations. ...entertainment industry.       Additional resources on the Addgene Blog Learn about careers in science communication...

...Protocols Check Out How Addgene Develops Lab Management Software Resources on Addgene.org Find Protoctols ... and do not necessarily represent the views of Addgene. I think it’s safe to assume that anyone reading...100percentSDS.     Additional Resources on the Addgene Blog Learn How to Keep a Lab Notebook for Bioinformatic...

...limited distribution. We hope that will change with Addgene’s expansion into plant synthetic biology. There ...tool for plant engineering, please share it with Addgene to both build the plant synthetic biology community...on Twitter Like RevBio on Facebook Read more Addgene blog posts about synthetic biology ...

... over 3,000 eligible AAV cargo plasmids in the Addgene repository to be packaged in your choice of five..., read on.  Expanding our viral vector service Addgene has offered in-stock viral vectors since 2016, ...scientific support team is always available at help@addgene.org.  We hope you find this service useful in your...

...Markson et al., 2024), developed and deposited at Addgene by Arlene Sharpe’s lab, enables in vivo screens...10.1084/jem.20230699. Additional resources on the Addgene blog X-CHIME: Context-Dependent Germline Knockout...

...Posts Last month there was plenty of excitement at Addgene. Our new viral service has begun to pick up steam... Lego Movie). October was no less busy for the Addgene blog which once again had record readership with...

...Resources on Addgene.org Find tools for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's plasmids...work is required, several vectors can be found at Addgene that could serve as an excellent start (#22988,...many tools for shRNA in mammals are available at Addgene. Identifying actively silenced plants Once you’...APT-based RNAi experiment are now available on Addgene. These include the construct that allows for easy...10.1104/pp.18.00828 Additional resource on the Addgene blog Browse our blog posts about plant biology...

...resources on Addgene.org  Addgene's Plasmids 101 eBookAddgene's Molecular Biology ReferenceAddgene's Restriction... performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our uncut... the short-read Illumina MiSeq sequencing that Addgene uses.) However, when some researchers received ...described in this article.   Long-read sequencing Addgene uses Illumina MiSeq NGS to perform whole plasmid...Restriction Digest Protocol More resources on the Addgene blog:  Plasmids 101: What is a plasmid? Plasmids ... in the comments below or contact us at help@addgene.org. Resources and References References Bacolla...

...Science Communication Resources at Addgene.org Check out Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...small tasks (like... preparing plasmids to send to Addgene). Following my advice from above, you should probably... in the comments! Additional Resources on the Addgene Blog Management for Scientists eBook Get Advice...

...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything...content on many of the requested topics to the Addgene blog The blog has many other excellent posts on...and happy to help. Additional Resources on the Addgene Blog: 10 Ways to Share Your Science 6 Tools & ...How We're Sharing Science through Video with the Addgene Youtube Channel Check Out Vidoes of Gender & Science...

... for the generation of human disease models." Addgene Outreach Scientist and fellow blogger Caroline ...CRISPR Plasmids? CRISPR-Cas9 Plasmids available at Addgene CRISPR-Cas9 References, Protocols, and more References...

...pigment. Find plasmids for blue-white screening at Addgene! Some bacterial strains contain lacZ in their genome... troubleshooting Sanger sequencing results. At Addgene, we now use next-generation sequencing during our...the correct clone. Additional resources on the Addgene blog Get all the plasmid basics in our Plasmids...molecular biology protocols and tips Resources on Addgene.org Learn more about Sanger sequencing Read the ...

...desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent protein...stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems.../jbc.m117.777722 Additional resources on the Addgene blog Browse all of our CRISPR blog posts Find ...with our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

...resources on the Addgene blog Find our synthetic biology blog posts Resources on Addgene.org Find plasmids... pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a stable Cas expression...” by Benoit Giquel, an outreach scientist from Addgene. The talk highlighted the importance of reagent...

...Paolo Colombi, a product development scientist at Addgene. Cloning can be quite an arduous process. The PCR...currently the product development scientist at Addgene. In the lab he develops new assays and processes...possible aspects.     Additional Resources on the Addgene Blog Browse our Plasmids 101 blog posts Read blog...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ...

...added phosphoserine. A community resource Like Addgene, GCE4All is a community resource which depends ...researchers. One way of doing that is by using Addgene’s Blue Flame award to identify highly requested ...the plasmids and bacterial strains (available at Addgene for GCE work. Plus, keep an eye on our blog – we...