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...article can be obtained through Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885...At Addgene we're continually impressed with the amazing plasmid technologies developed by our community... Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche et al., Nat ...brilliant SunTag system plasmids are available through Addgene and are already very popular. Use these plasmids...pathways (Figure 2). They are all available now at Addgene. 1 Lungu et al., Chem Biol. 2012 Apr 20;19(4..., and other plasmids from this article through Addgene. Nihongaki et al., Chemistry and Biology. 2015...human genes. The plasmids are all available at Addgene ready to light up the genome of your favorite cells...

... & Small Molecule Fluorophores. Addgene Blog. https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores... is now the most popular self-labeling tag in Addgene’s collection. CLIP-tag: Also in 2008, Kai Johnsson...are the most popular self-labeling enzymes in Addgene’s collection, so you may be able to get a plasmid...ligand to use for your experiment, and explore Addgene's collection to find self-labeling fusion proteins...10.1021/acs.biochem.1c00258 More resources from Addgene Better Dyeing Through Chemistry Illuminating ...GCE4All: Making Genetic Code Expansion Accessible Addgene's Genetic Code Expansion Collection ...

...conferences Resources on the Addgene website Become of a member of the Addgene depositor community Learn... science communication.       Resources on the Addgene blog Read other posts on scientific communication...Learn about careers at Addgene ...

...partner with Addgene. What are the most important issues for our partners and how can Addgene work with ...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by highlighting...Richard D. Lewis Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

...informed this section.  Find mStayGold plasmids at Addgene here!   Ando, R., et al. (2023). StayGold variants...primate brains with brilliance By Hridaya Patel (Addgene co-op) Researchers in the Gradinaru lab have developed...good option for essentially all applications. Addgene’s plasmid collection includes over 10,000 plasmids...both of which are also available in trial sizes. Addgene recommends these recombinant antibodies for use...

... Resources on Addgene.org Addgene’s Viral Services Addgene’s Virus Protocols Addgene’s Chemogenetics ...Chemogenetics Guide Resources on the Addgene blog Viral Vectors 101: Chemogenetics Viral Vectors 101: Optogenetics...

... and references Resources on addgene.org Addgene's CRISPR Guide Addgene's CRISPR 101 eBook CRISPR resources...nearby genes (Figure 1A-C). Find CRISPR plasmids at Addgene! Knock-in: Introducing tags or stop signals Once...resources More resources on the Addgene blog RNA Editing with Cas13 Targeting RNA with Cas13a (C2c2) Cas13d...

... do not necessarily represent the views of Addgene. Addgene performs Sanger sequencing on select regions...Twitter - Tweet suggestions and tips with #NgAgo The Addgene Blog - Leave suggestions and tips in the comments...PubMed Central PMCID: PMC4343198. Resources on the Addgene Blog Learn More about NgAgo Enhance CRISPR Targeting...comments section below or contact blog@addgene.org if you'd like to write a more detailed post It may take...Cpf1 Stacks Up to Cas9 and NgAgo Resources on Addgene.org Request NgAgo Find CRISPR Resources Catch Up...

...where the gene is expressed. Click here to view Addgene’s collection of empty backbones for constructing... such as FRET, calmodulin, and others. Review Addgene's collection of fluorescent biosensors here. Optogenetics...OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection: Expression constructs ...microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide Note: A. Max Juchheim...

...’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook...CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease, or both. Applications...

...Human Cell Differentiation Molecules group Addgene Data Hub Any (see notes) Any Data repository...repository Only accepts data for antibodies in Addgene’s repository  IBEX Knowledge-Base  Any IBEX...

...the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was unsuccessful. Nothing more ...pMal-T-Avi-His/BirA plasmid now available through Addgene. We have successfully used this plasmid to biotinylate...cell signaling.     Additional Resources on the Addgene Blog Check out other Hot Plasmids Find Additional...Posts on Plasmid Technologies Resources at Addgene.org Learn about Popular Plasmids in the Repository...

...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...These vectors listed below should be released at Addgene in the next 6 months, pending quality control. ....1-WPRE-SV40  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...

...For example, the many resources available on the Addgene website didn't come into being through the actions... Managerial Skill" Additional Resources on the Addgene Blog Read the Rest of the Management for Scientists... Individual Development Plan Resources on the Addgene Website Are You a Molecular Biologist? Find Plasmids...

...received matches the expected sequence. Here at Addgene, we use NGS-based quality control to confirm the...necessary but also an easy process. Please vist Addgene's resource for plasmid verification to find additional...DNA gel.  More Plasmid Eductional Resources: Addgene's Molecular Biology Reference Find More Protocols...

...ordering plasmids containing them from Addgene. If a plasmid at Addgene contains a gene that’s been codon ... for example). As many plasmids available from Addgene now have full sequence data, we recommend directly...PMCID: PMC2840511. Additional resources on the Addgene blog Plasmids 101: protein expression Plasmids...Plasmids 101 featured topic page Resources on Addgene.org Molecular biology reference pages Genetic code...

... Joanne Kamens Resources at Addgene.org Find Open Positions at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...that person before ever asking them for work. Addgene’s very own Joanne Kamens, a strong advocate for ...Scientists on docjobs.com Additional Resources on the Addgene Blog Check Out Career Topic Page Tips on Writing...

...Technical Support Calls Resources at Addgene Visit the Addgene Careers Page Check Out Our Career Videos...post. Klaus Wanisch is an Outreach Scientist at Addgene. These days he's doing a lot of science communication...cellular therapies.     Additional Resources on the Addgene Blog Read What Do I Do Now? Academic v. Non-Academic...this overall rewarding role. Learn about Other Addgenies and Their Careers in Our Career Videos What's ...

...Resources at Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All ...and used in assays for over half a century; the Addgene blog has an excellent primer and tips from experts..., Pages 1348-1353. Additional Resources on the Addgene Blog Tips for Using FRET in Your Experiments Learn...

...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts  ...Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ... found this blog helpful be sure to check out Addgene’s blog for other antibody topics.     References...