We narrowed to 592 results for: arl

1. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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   ...Medicine at the University of Washington. She is particularly interested in science communication and in vitro...

2. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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   ...the “elegans” methods outlined in this post – particularly the use of co-selection methods – could greatly...

3. RNA Interference in Plant Biology: New Tools for an Old Favorite

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   ...silencing plants. Non-destructive methods, most popularly with a fluorescent reporter, directly couple silencing...

4. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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   ... or excise a gene that is located between them. Early recombinase-dependent vector designs used a lox-...

5. Deep Dive: Statistical Tests (Comparisons)

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   ...Groups with Ordinal Dependent Variables: A Monte Carlo Simulation Study. Front. Appl. Math. Stat. Volume...

6. Sequencing Options for CRISPR Genotyping

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   ...25566793. PubMed Central PMCID: PMC4349094. 3. Bell, Charles C., et al. "A high-throughput screening strategy...

7. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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   ...spectrometry to identify the interacting proteins. Similarly, in ChIP-seq assays, chromatin fragments that ...

8. Which Fluorescence Microscopy Technique is Best for Me?

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   ...tissues of this size. For example, it could take nearly two months to image an entire mouse brain with ...

9. Custom CRISPR Screens & the Green Listed Software

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   ... studied cellular behavior. This approach is particularly suitable for in vivo screens, where CRISPR modified...

10. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    ...leading to dimmer fluorescence. In fact, many of the early reversible PA-FPs also display this “negative” photoswitching...

11. Antibodies 101: Introduction to Antibodies

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    ...   Enzyme-linked immunosorbent assay (ELISA) Similarly, ELISA is used to detect the presence of a single...

12. Antibodies 101: Designing Your First Flow Panel

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    ...Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects ...

13. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    ...stable signal over extended periods, which is particularly beneficial when studying dynamic processes like...

14. Typing CRISPR Systems

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    ...about 90% of identified CRISPR-Cas in bacteria and nearly 100% in archaea, though Class 1 remains largely...

15. Special Delivery: Fluorophore Targeting for FRET Studies

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    ...80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling...

16. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    ...38]. In 1987, Davis et al. reported one of the earliest examples of transdifferentiation in vitro where...

17. CRISPR 101: Multiplex Expression of gRNAs

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    ...plasmids from this paper at Addgene. 12. Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE...

18. Antibody Validation for Flow Cytometry

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    ...different providers for the same clone. This can be particularly useful when designing larger multicolour panels...

19. Delivery Methods for Generating iPSCs

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    ...5. González, Federico, Stéphanie Boué, and Juan Carlos Izpisúa Belmonte. "Methods for making induced pluripotent...

20. Technical Design of a Western Blot

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    ...important part of your western is your antibodies, particularly your primary antibodies. We always recommend...