We narrowed to 599 results for: arl

1. Plasmids 101: Dimers and Multimers

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   ...plasmid multimerization. Plasmid Multimerization Early studies on plasmid topology recorded plasmid multimerization...

2. Antibodies 101: The Basics of Western Blotting

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   ...could contain unintended cross-reactive proteins. Similarly, if you vary the experimental conditions from ...

3. Technique: Probe Phage Genomes for Host Binding Proteins

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   ... and a few proteases knocked out making them particularly useful for protein production), then dilute ...

4. Finding Your Perfect Job After University

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   ... a type of pediatric kidney cancer. This was particularly interesting as I had just completed my thesis...

5. Building Global Connections with the International Mentorship Program USA-EUROPE

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   ...President from the non-profit IMFAHE, Inc. She is particularly interested in breast cancer and metastasis as...

6. Important Considerations When Using AAVs

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   ...receptors (i.e. chemogenetics vs. optogenetics) I’m particularly interested in using chemogenetic and optogenetic...

7. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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   ...the “elegans” methods outlined in this post – particularly the use of co-selection methods – could greatly...

8. RNA Interference in Plant Biology: New Tools for an Old Favorite

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   ...silencing plants. Non-destructive methods, most popularly with a fluorescent reporter, directly couple silencing...

9. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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   ... or excise a gene that is located between them. Early recombinase-dependent vector designs used a lox-...

10. Deep Dive: Statistical Tests (Comparisons)

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    ...Groups with Ordinal Dependent Variables: A Monte Carlo Simulation Study. Front. Appl. Math. Stat. Volume...

11. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    ...Medicine at the University of Washington. She is particularly interested in science communication and in vitro...

12. Sequencing Options for CRISPR Genotyping

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    ...25566793. PubMed Central PMCID: PMC4349094. 3. Bell, Charles C., et al. "A high-throughput screening strategy...

13. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    ...spectrometry to identify the interacting proteins. Similarly, in ChIP-seq assays, chromatin fragments that ...

14. Which Fluorescence Microscopy Technique is Best for Me?

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    ...tissues of this size. For example, it could take nearly two months to image an entire mouse brain with ...

15. Custom CRISPR Screens & the Green Listed Software

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    ... studied cellular behavior. This approach is particularly suitable for in vivo screens, where CRISPR modified...

16. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    ...leading to dimmer fluorescence. In fact, many of the early reversible PA-FPs also display this “negative” photoswitching...

17. Antibodies 101: Introduction to Antibodies

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    ...   Enzyme-linked immunosorbent assay (ELISA) Similarly, ELISA is used to detect the presence of a single...

18. Typing CRISPR Systems

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    ...about 90% of identified CRISPR-Cas in bacteria and nearly 100% in archaea, though Class 1 remains largely...

19. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    ...stable signal over extended periods, which is particularly beneficial when studying dynamic processes like...

20. Antibodies 101: Designing Your First Flow Panel

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    ...Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects ...