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We narrowed to 641 results for: ato

Showing: 561 - 580 of 641 results
  1. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...hypothesis: proper controls! For every experiment, an investigator needs a standard against which the results can...
  2. AAV Production in HEK293 Cells

    Type
    Protocol
    ...before putting the CS5 in the incubator. Return the CS5 to the incubator for 96 h. This incubation time...Hemocytometer or cell counter Ice bucket CO 2 incubator Pipet controller Hazardous waste container pH ...pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents Adherent HEK293T cells (...media and mix. Seed all cells in 1 CS2. Return to incubator for 48 h. Trypsinize and resuspend cells from ... with a total volume of 850 mL. Return to the incubator for 24 h–36 h. Proceed with transfection: Calculate...2% HI-FBS. Mix well. Take the CS5 out of the incubator and pour the media into a waste container. Carefully...evenly between the layers. Return the CS5 to the incubator for ~72 h. Harvest cells and media by tapping ...
  3. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...dCas9, zinc finger proteins, and transcription activator-like effectors (TALEs), with fifteen total variants...
  4. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...introduce or remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter...
  5. Evolution of Lab Techniques

    Type
    Blog Post
    ...DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress...
Showing: 561 - 580 of 641 results