Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 819 results for: cell

Showing: 561 - 580 of 819 results

  1. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...Class 2 CRISPR-Cas System. Cell 163:759–771 . https://doi.org/10.1016/j.cell.2015.09.038 Additional resources... also detect low-frequency cancer mutations from cell-free DNA fragments as well as health-related single...similar types of HPV, HPV16 and HPV18, from human cells, at atomolar levels, within one hour. DETECTR thus...of Diverse Class 2 CRISPR-Cas Systems. Molecular Cell 60:385–397 . https://doi.org/10.1016/j.molcel.2015.10.008...

  2. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ... brain regions. You can partially control which cells are successfully infected by an AAV by altering ...control where your gene of interest is expressed.  A cell-type specific Cre can provide you with that control...

  3. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...multiple plasmids in one cell, it might be that you can't stably maintain them in the cell. This blog post features...

  4. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...mixture of two base editor enzymes in mammalian cells. Read the paper in bioRxiv Find Target-ACE   CRISPR...bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages like reduced off-target activity ...

  5. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...pathways that are frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis...pathway. “The idea is that if you introduce them into cells one at a time, you could identify those that confer...

  6. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... GFP, enabling you to select cells with high GFP expression. These cells have the highest levels of Cas9... on the same plasmid, you’ll make sure that each cell that gets the plasmid contains all of the desired...gRNA, which causes hypopigmentation, thus marking cells that have undergone genomic modification. In this..., et al. “Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system.” Scientific...

  7. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...lack uracil, but contain methionine. (This allows cells carrying nonfunctional as well as functional MET... is available at our Investigations in Molecular Cell Biology Website. Many thanks to our guest blogger...right) has taught courses in biochemistry, molecular cell biology and genetics at Boston College. She is particularly...

  8. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ... monitoring sensitized emission in living plant cells." (2013). PubMed PMID: 24194740. PubMed Central ...-molecule FRET for imaging biomolecules in live cells (Sustarsic and Kapanidis 2015), which may lead to...understanding biomolecular structure and dynamics in live cells." Current opinion in structural biology 34 (2015...fluorescent proteins available. Fortunately, several excellent reviews have collated data from various papers... alters the FRET efficiency. Detecting certain cellular changes with an intermolecular probe is often ...Miyawaki et al., 1997), was designed to measure intracellular calcium and published in 1997. Since that time...of labs to answer sophisticated questions about cellular processes. Currently, FRET experiments can probe...

  9. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...currently doing this using two approaches: single-cell RNA-sequencing to identify neuronal class and deep...WDDD aims to acquire quantitative data on all the cell divisions of the C. elegans embryo when all essential...Wang et al., 2018).” With the split system, only cells that have both halves of cGAL can drive expression...

  10. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...Drosophilia Plasmid pRDB_051 (for stable fly cell lines; attL and attR sequences for genome integration...Drosophilia Plasmid pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration...rapid assessment of emerging CRISPR technologies. Cell Genomics, 4(3), 100519. https://doi.org/10.1016/...

  11. Editor's Choice, August 2016

    Type
    Blog Post
    ...or G->A transitions in a target sequence in live cells. This technique uses catalytically inactive CRISPR...and other means of generating mutations in live cells are all discussed further in this informative post...Clearly we have all of our writers to thank for the excellent content they’ve churned out in the last month....

  12. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...small Cas protein, is active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered ...demonstrating that CasX is active in both E. coli and human cells. Using the structure of CasX at eight different ...

  13. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ... check viral transgene expression in transduced cells whenever possible. This post will describe our workflow...fragments of DNA from the helper plasmids or the cell genome can be packaged inside the vectors during...earlier, it is common to find DNA from the packaging cell genome, bacterial genomes, cloning vectors, and ...

  15. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...tag and small molecule. It does work in mammalian cells, mice, and even yeast, though (we have a kit!). ...and complete degradation of proteins in mammalian cells. Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350...Tags Plasmids for Endogenous Gene Tagging in Human Cells Antibodies 101: Epitope Tags  ...uses a small molecule, a tag, and the endogenous cellular degradation machinery to function. The dTAG is...

  16. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ... localization of that biomolecule inside living cells. FPs classified by the emission color (emission ...proteins and their applications in imaging living cells and tissues”  Physiol. Rev. 90:1103. Wu et. al. ... nuclear localization, and dynamics” Curr. Opin. Cell. Biol. 23:310. .... Therefore, these can be used as “timers” for cellular processes following their activation. The four...Biosensors: Throwing color lights on molecular and cellular processes” Curr. Protein. Pept. Sci. 9(4):338....

  18. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...predict that we’d be able to edit RNA in mammalian cells with CRISPR? I certainly didn’t, but now we can ...Improve HDR Efficiency for CRISPR Editing in Human Cells Day 3: Cpf1: Cpf1: A New Tool for CRISPR Genome ...

  19. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...mismatched translation rates and potential effects on cell growth, even hosts producing alternative tRNAs may...inclusion bodies that will generally segregate with the cell pellet during purification procedures. Even if you...a means to fine-tune gene expression." Molecular cell 59.2 (2015): 149-161. PubMed PMID: 26186290. PubMed...reasons include: Metabolic pressures - it takes cellular resources to produce tRNAs that recognize different...

  20. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...score (KO-score) which represents the proportion of cells that have either a frameshift or 21+ bp indel. This...Following delivery of CRISPR components into target cells, genomic DNA from both edited and unedited (control... score. KO Score - Represents the proportion of cells that have either a frameshift or 21+ bp indel. This...
Showing: 561 - 580 of 819 results