...fusion protein binds to glutamate, conformational changes lead to an increase in fluorescence intensity from... from the inserted GFP. After characterizing iGluSnFR in vitro, showing that it is selectively activated...response than its predecessors. The detailed characterization of the new variants is available here. Upgrades...

...throughput functional characterization of specific edits to the genome. Such characterization can be done in ...The RNAi process had just been discovered, the mechanism was barely understood.” Doench’s graduate work...

...non-academic one, one of the biggest changes (and perhaps challenges) is the need to present yourself using...studied astronomy or bioinformatics data, then the chance is that you have handled large data sets and are...

... for them to be imaged in distinct fluorescent channels on the microscope. With the proliferation of fluorescent...the best protein for a given application is a challenge. To help keep you abreast of the latest fluorescent... abundant proteins, with the blue and infrared channels used for more abundant proteins or marking compartments...

...cells Blocks polypeptide synthesis at 80S; inhibits chain elongation 100-800 ug/mL Hygromycin B hygB HeLa...cells Blocks polypeptide synthesis at 80S; inhibits chain elongation. 50-500 ug/mL Puromycin pac HeLa, 293HEK...Jurkat T cells Inhibits protein synthesis; premature chain termination 1-10 ug/mL Zeocin Sh bla HeLa, NIH3T3...

...management, the journey can be as exciting as it is challenging. How do you prepare for this shift and navigate...experiences, and practical advice for overcoming challenges. Experimenting with leadership opportunities...'ve witnessed several colleagues do this while changing industries and positions. Some people discover...

...picked up by the sensors. You can adjust this by changing the voltage for the particular fluorophore prior...protein into your cells (visualized via the FITC channel) and you are trying to see what percentage of total...expression, using a histogram plot and MFI values to characterize your samples may allow for better interpretation...

...our understanding of what accessibility is would change over the next twenty years. But learn we did! ...scientific paper, you may feel it’s overwhelming or challenging. So instead, let’s focus on practicing with a...when I’m done? What are we trying to fundamentally change about their knowledge?    Here, this image is being...

...IPTG, the lac repressor undergoes a conformational change that removes it from lacO sites within the promoter...there is very little crossover. The induction mechanism must also be compatible with your experimental...The Promoter Region Plasmids 101: Cre-lox Light-Switchable Two-Component Systems for E. coli Browse other...

...knew that the best days in the classroom are characterized by intense student engagement and joy, rather...development comes from the Caltech lab of Professor Richard Murray. Murray’s lab uses cellular extracts to ...collaboration has resulted in a new BioBuilder book chapter and an illustrated, downloadable classroom poster...

...biological phenomenon, there’s a reasonable good chance that Addgene can help. Though it would be difficult...Kerstin Bystricky Courtney Hodges Sharon Dent Archa Fox Elaine Fuchs Kai Ge Robert Kingston Thoru ...Julie Ahringer Alexander Stark Bas van Steensel Richard Young Yi Zhang Leonard Zon  You!     Chromatin-related...

...experimental approaches. One example is CasX, characterized by the Oakes and Doudna Labs, which is both ...research and potentially treatments. Cas12b, characterized by the Zhang (Strecker et al., 2019) and Li ... relative to Cas9. As scientists continue to characterize CRISPR nucleases from a variety of sources, ...

...imaging! These eNL constructs use the brightest characterized luciferase, NanoLuc, and its substrate furimazine...Nagai laboratory has a done a wonderful job characterizing Nano-lanterns and eNL for many different applications...eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al. also envision...

...the presentation and Q&A session on our YouTube channel. And with no more ado, the questions!  AAV Prep...prohibitive problem? A: Re-administration is a challenge for AAV due to the development of neutralizing... in diseases which may be linked to epigenetic changes, such as multiple sclerosis?  A: Basic science ...

...non-coding portion of the genome. Mendenhall and Richard Myers of HudsonAlpha (where Mendenhall is also ...transcription factor. The FLAG antibody has been well characterized and the system allows you to add this same antibody... Besides being fast and efficient, what other challenges does this system help to solve? Myers: Only a...

...contributed by David Wyatt and Dale Ramsden, UNC at Chapel Hill. One advantage to using the CRISPR/Cas system...heterogeneous. There is consequently about a two-thirds chance of causing a frameshift mutation. Of some importance...the Lineberger Comprehensive Cancer Center at UNC Chapel Hill.   References Bennardo N, Gunn A, Cheng A,...

...Developmental Editor at F1000Research.  With many changes taking place in the world of science publishing... you look unprofessional and doesn’t help your chances of getting your manuscripts published in the future...manuscript full of spelling errors will not have a great chance of getting published. In summary, it is a good...

...for genetic analysis, as well as an efficient mechanism of homologous recombination to facilitate simple...primarily pertains to ORIs in budding yeast, Saccharomyces cerevisiae; however, we’ve also noted some features...required for the replication of fission yeast, Schizosaccharomyces pombe, vectors at the end.) In a previous...

.... In a recent issue of Cell Stem Cell, Musunuru, Chad Cowan and their colleagues describe a much more ...genome editing tool with which one can introduce changes into the genomes of mammalian or other cells. TALENs...for a particular sequence, there is a much better chance they will bind that sequence. That’s the revolutionary...

...Technical difficulties turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving... speaking is hard. Aside from the diving board charades, I doubt anyone but me remembers any of the incidents...opportunities, and gives your entire audience a chance to comfortably engage with you. If you can give...