We narrowed to 630 results for: emb

1. Anatomy of a Plasmid Page at Addgene

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   Blog Post

   ...provides useful information about how the vector was assembled, and enables requesting labs to utilize and modify...

2. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   Blog Post

   ...the cell. Can be used for generating transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified...

3. Summer SciComm Series: Modes of Communication

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   Blog Post

   ... limiting factor at multiple points. Before you embark on a project, do your best to understand what kind...

4. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

   ...’t work for all bacteria.   Zeocin  Zeocin is a member of the bleomycin family of antibiotics, which induce...

5. AAVs in Retinal Gene Therapy

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   Blog Post

   ...later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna™ the first ...

6. Transferable Skills Guide: Identifying Your Transferable Skills

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   Blog Post

   ...commitments, respond to any issues raised by team members, and plan for the future of the team. All while...

7. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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   Blog Post

   ... materials expired? Did you pipette carefully? Remember to discard flow-through solutions and use clean...

8. Antibodies 101: Producing Recombinant Antibodies

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   Blog Post

   ...system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (...

9. SciComm: Taking Science to Elementary and Middle Schools

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   Blog Post

   ... Science Night, a chance for parents and other members of the community to share their expertise with ...

10. CRISPR 101: Making a Knock-In Cell Line

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    Blog Post

    ...out your Cas9? It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a...

11. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

    ...mammalian cells. Like Cas9 nucleases, Cpf1 family members contain a RuvC-like endonuclease domain, but they...

12. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ...sensitive to toxicity from RNA editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development...

13. Antibodies 101: Immunoprecipitation

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    Blog Post

    ...adjusted depending on the sample. It is important to remember that sample lysates will also contain proteases...

14. Viral Vectors 101: Pseudotyping

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    Blog Post

    ...find tables and information on different viral membranes, tropism, and advantages and disadvantages: Table...

15. Viral Production at Addgene

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    Blog Post

    ...filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, ...

16. Plasmids 101: Plasmid Incompatibility

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    ...resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman et al., 2017)...

17. Neuronal labeling with Spaghetti Monster

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    Blog Post

    ... Flying Spaghetti Monster; the smFP structure resembles images of the deity (see comparison image above...

18. Transferable Skills Guide: External Collaborations

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    Blog Post

    ...feeling weird and started to be fun. I just had to remember to approach the relationship with the perspective...

19. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post

    ...Remember the game show “The $25,000 Pyramid” where one player tries to get the other to guess a category...

20. Transferable Skills Guide: Career Planning Resources

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    Blog Post

    ...clearly a good idea to be diligent in your research, remember that you will not be a graduate student or postdoc...