We narrowed to 622 results for: erf

... vector.  Once the BP and/or LR reactions are performed, the next step is to transform competent E. coli...

...making it easier for comparable studies to be performed on a cell line even decades after it is initially...

...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...

...discovery, plasmid addiction systems have become a powerful tool to address a diverse set of biological inquiries...

... in your expression host of choice, you should perform a functional assay to make sure your protein isn...

...identified (Hart et al, Bassik et al). The library performed well in both screens, with the growth screen having...

...dilute the unintegrated plasmids (Fig. 2b). Next, perform a short (12-hour) induction with doxycycline prior...

...strand breaks it introduces makes it difficult to perform two or more integrations on the genome. Most recently...

...novel PAM recognition In 2015, Keith Joung’s lab performed a series of positive selection screens in bacteria...

...pluripotent stem cells By Jennifer Tsang CRISPR gene interference (CRISPRi) and gene activation (CRISPRa) are ...

... CM, Partridge SR, Iredell JR (2017) Plasmid interference for curing antibiotic resistance plasmids in...

...conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research Conference...

...discussed some of the general considerations for performing behavioral tests, but now we’ll focus on how ...

...together with information about the genome are powerful resources for investigating any biological process...

...in the laboratory mouse, most optogenetics is performed in this species. However, transgenic rat technology...

... expression from high-copy number plasmids and powerful promoters will greatly exceed that of any native...

...a good place to start when trying to pick your perfect promoter! Eukaryotic Promoters   Promoter   ...

...integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the synthetic...

...recruiting proteins to the cell membrane isn’t powerful enough, what about altering the chemical composition...

...weeds. Check out Joanne Kamens's "10 Steps to a Perfect Science Talk." 4. The chalk talk, simple in principle...