We narrowed to 639 results for: erf

1. Negotiating Work and Life: How to Find the Joy

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   Blog Post

   ... and/or your spouse won’t be at every school performance, chaperone every field trip or cheer at every...

2. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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   ...dilute the unintegrated plasmids (Fig. 2b). Next, perform a short (12-hour) induction with doxycycline prior...

3. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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   ...novel PAM recognition In 2015, Keith Joung’s lab performed a series of positive selection screens in bacteria...

4. Hot Plasmids - August 2020

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   ...pluripotent stem cells By Jennifer Tsang CRISPR gene interference (CRISPRi) and gene activation (CRISPRa) are ...

5. Deciphering the Mysteries of Behavior with Viral Vectors

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   ...discussed some of the general considerations for performing behavioral tests, but now we’ll focus on how ...

6. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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   ...together with information about the genome are powerful resources for investigating any biological process...

7. New Tools Enable CRISPRa for Neuroscience Applications

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   ...Fos, Egr3, and Arc. Thus, CRISPRa represents a powerful method to dissect the individual and shared contributions...

8. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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   ... plants are known to many as the only Monarch butterfly food. They have chemical pathways generating interesting...

9. CRISPR-based Genome Editing Systems For Editing in E. coli

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   ...strand breaks it introduces makes it difficult to perform two or more integrations on the genome. Most recently...

10. Plasmids 101: Plasmid Incompatibility

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    ... CM, Partridge SR, Iredell JR (2017) Plasmid interference for curing antibiotic resistance plasmids in...

11. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    ...conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research Conference...

12. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    ...difficult to obtain. These can then be used to perform cell therapy and to model diseases in vitro. Find...

13. Designing Your Chalk Talk for the Academic Job Interview

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    ... exciting. Practice presenting your chalk talk Perfect your story and delivery. Design simple figures ...

14. CRISPR-mediated Plant Base Editors

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    ...G)? You may wonder if a CRISPR-base editor can perform this change. However, there are no naturally known...

15. How to Be an Excellent Trainee

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    ...enough information there for you to be independent performing the technique you trained to do? Walk through...

16. QC Sequencing Technologies at Addgene

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    ...specified discrepancies. Any sequences that are a perfect match from this script can then continue in the...

17. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post

    ...vector was useful for the requestors or how it performed. Addgene launched our AAV Data Hub as a community...

18. Hot Plasmids Spring 2024

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    ...Ace2N-mNeon also led to similar improvements in performance.      Figure 4: Structure of Voltron...

19. Tips for Writing a Good Cover Letter

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    Blog Post

    ...for Scientists Series Get Tips on Finding Your Perfect Job after Graduation Additional Resources Hamilton...

20. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ...discovery, plasmid addiction systems have become a powerful tool to address a diverse set of biological inquiries...