We narrowed to 637 results for: erf

1. Plasmids 101: Protein tags

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   ...co-IP, IF, flow -cytometry; can occassionally interfere with protein folding HBH HHHHHHAGKA GEGEIPAPLA...

2. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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   ...recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method to suppress PCR amplification...

3. Negotiating Work and Life: How to Find the Joy

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   ... and/or your spouse won’t be at every school performance, chaperone every field trip or cheer at every...

4. Deciphering the Mysteries of Behavior with Viral Vectors

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   ...discussed some of the general considerations for performing behavioral tests, but now we’ll focus on how ...

5. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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   ...together with information about the genome are powerful resources for investigating any biological process...

6. New Tools Enable CRISPRa for Neuroscience Applications

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   ...Fos, Egr3, and Arc. Thus, CRISPRa represents a powerful method to dissect the individual and shared contributions...

7. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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   ... plants are known to many as the only Monarch butterfly food. They have chemical pathways generating interesting...

8. Designing Your Chalk Talk for the Academic Job Interview

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   ... exciting. Practice presenting your chalk talk Perfect your story and delivery. Design simple figures ...

9. Twenty Years of Sharing: Addgene's Viral Vector Service

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   ...vector was useful for the requestors or how it performed. Addgene launched our AAV Data Hub as a community...

10. Hot Plasmids Spring 2024

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    ...Ace2N-mNeon also led to similar improvements in performance.      Figure 4: Structure of Voltron...

11. Tips for Writing a Good Cover Letter

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    ...for Scientists Series Get Tips on Finding Your Perfect Job after Graduation Additional Resources Hamilton...

12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    ...from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize the protein in mammalian...

13. Fluorescent Proteins 101: Aptamer Fluorophores

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    ...veggies) Though the aptabeacons can be used as powerful biosensors, the bound protein fluorophores and...

14. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...novel PAM recognition In 2015, Keith Joung’s lab performed a series of positive selection screens in bacteria...

15. Hot Plasmids - August 2020

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    ...pluripotent stem cells By Jennifer Tsang CRISPR gene interference (CRISPRi) and gene activation (CRISPRa) are ...

16. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    ...dilute the unintegrated plasmids (Fig. 2b). Next, perform a short (12-hour) induction with doxycycline prior...

17. CRISPR-based Genome Editing Systems For Editing in E. coli

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    ...strand breaks it introduces makes it difficult to perform two or more integrations on the genome. Most recently...

18. QC Sequencing Technologies at Addgene

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    ...specified discrepancies. Any sequences that are a perfect match from this script can then continue in the...

19. Plasmids 101: Plasmid Incompatibility

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    ... CM, Partridge SR, Iredell JR (2017) Plasmid interference for curing antibiotic resistance plasmids in...

20. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    ...conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research Conference...