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...will spend 10 or more years with a small number of formal advisors learning how to be a scientist. It is ...willing to talk by phone. Don’t use email to get information if you can help it. Observe as much as you can...If lab members, neighbors or alumni give you information about the PI that concerns you, don’t just brush...

...multiple plasmids used for various platform applications. For more information on CRISPR/Cas, check out Addgene...creatively to deliver a highly specific, programmable platform well-suited for various innovative biological ...

...collaboration without having to worry about proprietary information Work hours are long, but there is more flexibility...opportunities—projects end and layoffs occur, but good performers move around easily  Table 1: Taking it a Step... career with advancement in one company, good performers in for the long haul Limited movement in one...

...these pieces aren't always appropriate for your formal scientific communications, they are fantastic fodder...Second, the introduction needs to provide enough information so that the audience can understand the conflict...scientific problem) will also help you determine what information is necessary and what can be left out, which ...

...1970s by Alex van der Eb in the Netherlands. Transformation of HEK cells with adenovirus 5 DNA was postdoc...Resources References Shaw G., et al., Preferential transformation of human neuronal cells by human adenoviruses...0995fje Tan, E., et al., HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors...

...demonstrates that you have advanced writing and formatting skills, which are useful to many employers. Increasingly...Science Reporter Technical Writer Statistician Bioinformatician Science Writer Grant Writer Journal Editor...are willing to exercise these skills outside of a formal work environment also shows that you are passionate...

...Perhaps you have a paper, protocol, or other information that may be helpful or of interest to them. Don...you to someone who you need to speak to for informational or career purposes.  Don’t only talk about work... conference soon? Get tips here Learn about informational interviews ...

...cells transmit electrical information. Such voltage imaging can be performed in intact animals and offers...circulatory systems, but can also lead to the formation of atherosclerotic plaques. ApoB bound lipoproteins...

... DOI Citation Formatter Just enter the DOI of a paper and select your desired formatting style. This tool...to get those words out and onto the screen and transform your work into a paper or a grant. Here’s a list...

... like gel electrophoresis to separate DNA or transforming cells with green fluorescent protein, these ... into a host cell (through a process called transformation), essentially giving the cell all the instructions...hands-on educational activities using the BioBits platform, please get in touch with us! We’ll work with ...

...participants, clarification - new and useful information, and invigoration - a renewed energy for continuing.... This keeps our all-company meetings short, informative, and pleasant. Email: Sometimes, the right ...Before long, you will be running collaborative, informative, energizing meetings all your own. Additional...

...You can’t delegate the giving of feedback or performance review. You can’t delegate tasks assigned to ... take on all tasks that involve confidential information. No matter who you delegate to, or what tasks...ensure successful results.  Can you provide the information and resources needed for the assignment? Setting...

...uncut DNA (lane 2) shows 3 possible plasmid conformations, with relaxed and nicked marked with asterisks...make it easier for you to obtain a useful and informative diagnostic restriction digest. For your digest...fact that the circular DNA takes on several conformations the most abundant being: supercoiled, relaxed...

... to work with, time consuming, and minimally informative. In this blog post, we’ll introduce digital PCR...target DNA. Conventional thermal cycling is then performed to amplify the target using PCR. Instead of tracking...design such assays, in addition to testing their performance (2). Remember that although digital PCR is a ...

...variable heavy (VH) chain sequences. Fusion PCR was performed to create an amplicon that  was cloned into a ...frequency within functional light chain sequences. By performing a BciVI digest of the PCR products prior to cloning...improving access to useful research materials and information. References and Resources Additional Resources...

...therefore will require crosslinking, which uses formaldehyde or UV light to create strong but reversible ...actively interacting with, reducing background information. Your options for this step are sonication, which... with a PCR purification kit or with a phenol-chloroform extraction.    Fig. 1: Schematic overview...

... genes, researchers at the Genetic Pertubation Platform at the Broad Institute have expanded the genetic...-based method for screens. Cas12a’s ability to perform multiplex editing within a single expression cassette...used to create a genome-wide library capable of performing similarly to Cas9 screens in a compacted library...

...have been identified in the sea pansy (Renilla reniformis), click beetle (Pyrophorus plagiophthalamus),... 562 61 Luciferin ATP No Sea pansy (Renilla reniformis) Natural 481 36 Coelenterazine None No Click...biosensors where BRET reports on the change in conformation of a single protein. Considerations for luciferase...

...and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1 protocol. Pro-tip: ...trademarked Tet-On(R) sold by Clontech. For additional information, see the manual provided by the Weiderschain ...vector encodes both SpCas9 and your gRNA. It’s the former version of our sixth most popular plasmid pSpCas9...

... still in its infancy. A long timeline from transformation to testing and a lack of well-characterized...s made even more difficult by the fact that transformation methods have also been patented. In order to.... We will also be working with open source, transformation technologies like those pioneered by Ewen Mullins...