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  2. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...Perhaps you have a paper, protocol, or other information that may be helpful or of interest to them. Don...you to someone who you need to speak to for informational or career purposes.  Don’t only talk about work... conference soon? Get tips here Learn about informational interviews ...

  4. Science Career Options

    Type
    Blog Post
    ...demonstrates that you have advanced writing and formatting skills, which are useful to many employers. Increasingly...Science Reporter Technical Writer Statistician Bioinformatician Science Writer Grant Writer Journal Editor...are willing to exercise these skills outside of a formal work environment also shows that you are passionate...

  5. How to Lead a Great Meeting

    Type
    Blog Post
    ...participants, clarification - new and useful information, and invigoration - a renewed energy for continuing.... This keeps our all-company meetings short, informative, and pleasant. Email: Sometimes, the right ...Before long, you will be running collaborative, informative, energizing meetings all your own. Additional...

  6. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...You can’t delegate the giving of feedback or performance review. You can’t delegate tasks assigned to ... take on all tasks that involve confidential information. No matter who you delegate to, or what tasks...ensure successful results.  Can you provide the information and resources needed for the assignment? Setting...

  7. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...uncut DNA (lane 2) shows 3 possible plasmid conformations, with relaxed and nicked marked with asterisks...make it easier for you to obtain a useful and informative diagnostic restriction digest. For your digest...fact that the circular DNA takes on several conformations the most abundant being: supercoiled, relaxed...

  8. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...have been identified in the sea pansy (Renilla reniformis), click beetle (Pyrophorus plagiophthalamus),... 562 61 Luciferin ATP No Sea pansy (Renilla reniformis) Natural 481 36 Coelenterazine None No Click...biosensors where BRET reports on the change in conformation of a single protein. Considerations for luciferase...

  9. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...variable heavy (VH) chain sequences. Fusion PCR was performed to create an amplicon that  was cloned into a ...frequency within functional light chain sequences. By performing a BciVI digest of the PCR products prior to cloning...improving access to useful research materials and information. References and Resources Additional Resources...

  10. Antibodies 101: ChIP

    Type
    Blog Post
    ...therefore will require crosslinking, which uses formaldehyde or UV light to create strong but reversible ...actively interacting with, reducing background information. Your options for this step are sonication, which... with a PCR purification kit or with a phenol-chloroform extraction.    Fig. 1: Schematic overview...

  11. Hot Plasmids - February 2022

    Type
    Blog Post
    ... genes, researchers at the Genetic Pertubation Platform at the Broad Institute have expanded the genetic...-based method for screens. Cas12a’s ability to perform multiplex editing within a single expression cassette...used to create a genome-wide library capable of performing similarly to Cas9 screens in a compacted library...

  12. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1 protocol. Pro-tip: ...trademarked Tet-On(R) sold by Clontech. For additional information, see the manual provided by the Weiderschain ...vector encodes both SpCas9 and your gRNA. It’s the former version of our sixth most popular plasmid pSpCas9...

  13. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ... to work with, time consuming, and minimally informative. In this blog post, we’ll introduce digital PCR...target DNA. Conventional thermal cycling is then performed to amplify the target using PCR. Instead of tracking...design such assays, in addition to testing their performance (2). Remember that although digital PCR is a ...

  14. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ... still in its infancy. A long timeline from transformation to testing and a lack of well-characterized...s made even more difficult by the fact that transformation methods have also been patented. In order to.... We will also be working with open source, transformation technologies like those pioneered by Ewen Mullins...

  15. Addgene: The First Twenty Years

    Type
    Blog Post
    ... to support sharing of CRISPR constructs and information across the globe.  “Read the Addgene guide: https...barrier for depositors and requestors alike. We transformed this process by aligning institutions with standardized...the Neuroscience AntiBody Open Resource (NABOR) platform, supported by an NIH BRAIN Initiative. We also...

  17. To Each HIS Own

    Type
    Blog Post
    ...methods where binding relies on a specific protein conformation, the polyhistidine-metal ion interaction is ...this one developed by Dr. James Trimmer’s lab, to perform assays that they otherwise would not be able to...polyhistidine tags. For more antibody-related information, sign up to receive alerts for antibody posts...

  18. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...as “sample,” “material,” “item,” and “format” all received formal definitions in our lexicon. We created...container, when applicable Any other relevant information Prescribing an official name to all of our plates...

  19. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...relevant host biomarkers. The lab validated their platform on clinical stool samples by comparison to qPCR...Gilliamella apicoli. The plasmids could also be transformed into Alphaproteobacteira, Betaproteobacteria,... al. "A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers...
Showing: 561 - 580 of 961 results