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We narrowed to 976 results for: lars;

Showing: 561 - 580 of 976 results

  1. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...from the David Liu and Dali Li labs reported a similar tool shortly thereafter, using mouse instead of...the base editor. Black arrows indicate natural cellular processes. Created with BioRender.com. The...mitochondrial base editing to base editors optimized for particular species and more, base editors are getting smaller... programmable T-to-G and T-to-C base editing. Molecular Cell, 84(7), 1257-1270.e6. https://doi.org/10.1016...

  2. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...  Physical titer is typically calculated by two popular PCR-based methods: Quantitative PCR (qPCR) Digital...generating a standard curve of known concentration. We regularly make and validate fresh plasmid standards. We...). However, because purified AAV preps contain similar and low levels of background protein and chemical...email us and we can do our best to send you a particular lot (if we still have it available). References...

  3. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...so great. If we all came from similar backgrounds, we would have similar cognitive biases and this would... information. It is quite possible that the clarifications lead to the story being scrapped or broken ...While writing the code, we may need additional clarification and again we collaborate with the Product Team...

  4. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ... the symptoms of mesothelioma are usually very similar to the symptoms of other diseases, such as asthma...diagnosis indicate, for Stage 3, that the cancer is large in size and may have spread while Stage 4 indicates...with new ways to discover the cancer earlier, in particular before it starts to spread beyond its point of...tumors. Studies have shown that bevacizumab is particularly effective when used in conjunction with standard...

  5. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...transcription factors, therefore promoters play a large role in determining where and when your gene of ...between cell types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...Repressible Gets turned off with high levels of cellular tryptophan. lac General expression Promoter ...

  6. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This... enough for all applications. To resolve this, similar systems have been developed for tracking various...animals only permits visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila...making this system useful for visualization of cellular extensions, such as neurons in the fly visual ...

  7. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...classes and resources that can provide details and vocabulary for this skill. What this post may do is help...something should be communicated is necessary in a large organization, because confirming that every person... I don’t just rely on what I know about those particular teams at that given time. I think about how previous... previous iterations of those teams resolved similar issues in the past, or how completely different teams...

  8. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ... you rule out errors like uneven pipetting or irregular membrane transfer. Figure 1: A loading ...important to validate these assumptions for your particular experiment and choice of loading control. First...Ubiquitously expressed proteins like β-actin or GAPDH are popular choices for loading controls. These are often called...in many cases, you should validate it for your particular setup. We recommend starting by searching the...

  9. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...accomplishment while the longer tasks will give you larger payoffs later. Don’t be afraid to accomplish some...ve also recently started using Trello to manage larger, multi-person projects. Day-to-day, flexible to-do...below): Read new Zhang Lab Paper Find plasmids similar to those in the paper Ask Mary G. about her thoughts...how much time you can possibly handle doing a particularly annoying task and then dedicating that much ...

  10. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...that strongly binds the glycoprotein, avidin or a similar protein, streptavidin. This strong interaction ...bait protein can even target BirA* to specific subcellular locations, such as the nuclear envelope, allowing...29169465.  Ge, Yun, et al. "Enzyme-Mediated Intercellular Proximity Labeling for Detecting Cell–Cell Interactions...smaller biotin ligase for BioID proximity labeling. Molecular biology of the cell, 27(8), 1188-1196. PubMed ...

  11. March for Science

    Type
    Blog Post
    ..., are limited by reality – a government cannot declare that gravity change direction and make things fly...comparison to 37% of the general public (5, 6). Similarly 90% of active research scientists believe climate... supporting it.  3) Linking scientists to one particular political party while alienating the other While...or politicians to interpret it for them. Other popular reasons to march include: General inclusion of...

  12. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...genes for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency...Table 2 provides a quick reference for some of the popular strains, their genotypes, and their primary use...Chloramphenicol High competency cloning and propagation of large plasmids, ligated DNA, and libraries, nalidixic ...coli and others are model organisms! Our Most Popular Plasmids 101 Posts: Origin of Replication The ...

  13. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...cells for mycoplasma (Link opens in a new window) regularly Do not over or under-grow your cells. Thaw a new...well (0 µL lentivirus, above) are dying. Perform regular media changes and monitor the growth of the cells...selection. It is important to monitor these cells regularly and replace the cell media. Cell death by some...cells in the culture, so it is important to do regular media changes and maintain optimal growth conditions... or removed from the culture, check the cells regularly to confirm transgene expression. As polyclonal... individual wells become confluent, expand into larger vessels. A confluent well of a 6-well dish can ...

  14. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... factors of phages) constitute one of the most popular classes of phage proteins to study. These are highly...characterizing sugars and sugar-containing molecules in molecular biology labs (for a review, see Simpson et al.... without causing issues associated with cloning large fragments. After fragmentation, repair sheared ends... and a few proteases knocked out making them particularly useful for protein production), then dilute ...

  15. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ... excited to announce the fourth edition of our popular eBook, Plasmids 101, is now available!   Plasmids...everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting tips. The...updated with twelve new sections, including our popular "Dimers and Multimers" post. It's also been re-...

  16. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...Our popular Antibodies 101 animation is becoming a TikTok series! Follow @addgene on TikTok for bite-...information! Link in bio. #STEM #antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene...Addgene We'll be posting these animations regularly from now until May, so follow us to get updates whenever...

  17. AAV Production in HEK293 Cells

    Type
    Protocol
    ... Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol can...efficient means to scale-up without having to handle a large number of T-175 flasks. Workflow Timeline Day 0:... (PES) filter system, Nalgene, 565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration...the amount of each plasmid needed to have a 1:1:1 molar ratio with 2 mg total DNA per CS5 Plasmid Plasmid...determine the total μg/bp we need to achieve a 1:1:1 molar ratio of each plasmid: 2000 μg / 24,961 bp = 0.08.... You are now ready to purify your prep . The clarified supernatant can be kept overnight at 4 °C before...

  18. Water Bath Protocol

    Type
    Protocol
    ...equipment that helps bring your materials to a particular temperature, catalyze chemical reactions such...such as restriction digests needed in molecular biology, and thaw cell lines. There are many different types...properly maintain the water bath, empty and clean it regularly. Determine the amount of water needed to fill ...out after your incubation is over. Note: If you regularly use a water bath in the lab, you can leave the...previous uses. However, you will still need to regularly clean out the water bath even if you are using...

  19. Centrifugation

    Type
    Protocol
    ...microfuge tubes used in labs, though some larger models can hold larger conical tubes. Depending on the centrifuge...They often spin at similar speeds to their smaller cousins, but can hold much larger containers. Ultracentrifuges...generally floor sized centrifuges that can hold larger containers, but they can also rotate at much higher...

  20. Lentivirus ddPCR Titration

    Type
    Protocol
    ...viruses per cellular genome: Since the cellular gene RPP30 is used as a reference to the cellular genome, ...other targets. Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) are ... lower or higher dilutions depending on their particular sample. This protocol was modified from the publication...#71205-3 Polybrene 10 mg/mL, Millipore, TR-1003-G Molecular Biology Grade Water, Hyclone, SH30538.02 ddPCR...
Showing: 561 - 580 of 976 results