We narrowed to 810 results for: mal
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TypeBlog Post...), but there are differences in tropism between animal species or even strains of the same species. What... to define high titer, then, is this: It’s the optimal number of viral particles per volume required to...
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Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post... frozen or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...in the uncut DNA; the rest of the digest appears normal and produces the expected fragments for that plasmid...transfected supercoiled plasmid DNA concatemers in mammalian cells. J Gene Med. 2009 May;11(5):444-53. doi:... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...of one AAV serotype with the capsid of another. Formally, pseudotypes are described with two numbers separated...using the genomic structure of AAV2, so they are formally called rAAV2/1, rAAV2/2, and so on, although we... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post...collaborative learning style at all. Finally I rotated in a small lab with a new PI. I realized that as a first born...collaboration? If you are considering a career at a small, teaching college you might want to consider less... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...its target states are only represented in a very small number of registers. Instead, one may use qPCR to... not be suitable for applications that require minimal cellular perturbation. Also, this method requires... -
Finding Your Perfect Job After University
TypeBlog Post... agencies and noticed a position available at a small science non-profit doing customer service (recruitment...this is a fairly unique situation, because it is a small and dynamic company and so it has the flexibility... -
Finding Your Science Policy Path
TypeBlog Post...societies, and industry through convening activities, formal lobbying, or strategy setting. The “little p” ...question may change over time and that’s perfectly normal! Action Items: Start a career journal during... -
A History of Genome Engineering in Popular Culture
TypeBlog Post...Moreau (1896) where he explores the concepts of animal testing and modifications. The premise of the novel... novel involves Dr. Moreau who creates human-animal hybrids. This book is published 30 years after the... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post... (22). Large purine:purine mismatches and most smaller pyrimidine:pyrimidine mismatches are typically ...screened per plate. The results indicated that the optimal buffer for Taq DNA Ligase contains 100 – 200 mM... -
Quick Guide to All Things Lentivirus
TypeBlog Post...development of viral vectors, gene transfer into mammalian cells often relied on chemical (CaCl2, cationic...If not using proper technique, there’s always a small chance that a researcher could infect him or herself... -
Transferable Skills Guide: Teamwork
TypeBlog Post...found that many researchers feel that they lack formal training in a variety of transferable skills. At...until the team really starts functioning (or malfunctioning). Clear lines of communication Few people actually... -
Antibodies 101: Flow Cytometry
TypeBlog Post...antibodies to pass through the cell membrane. Formaldehyde fixation creates cross-links between lysine ...Sometimes, users will combine methods and fix with formaldehyde first to freeze everything in place followed... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...ninety-nine cubits of Aristotle, nor would you mention my small error and at the same time pass over in silence ...she studies the development and regeneration of mammalian taste buds. Erin is also the President of Project... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post... activation system, but settled on a different optimal fusion protein combination. The optimized LACE ...optogenetic manipulation of CRISPR-based systems in animal models. Use Anti-CRISPRs to Turn Off Genome Editing... -
New Antibody Animation!
TypeBlog Post..., and the different antibody isotypes found in mammals. Subscribe to Addgene's YouTube channel ... -
#DeckTheLab to Win a Tasty Winter Treat This Holiday Season
TypeBlog Post...the entire lab (hint: you might need some mini marshmallows…). We’ll choose 4 submissions at random and ... -
The 12 Days of CRISPR
TypeBlog Post...Cas13 variants for targeted RNA degradation with minimal collateral effects🎵 On the 1st day of CRISPR, ... -
Expanded Access to Rabies Plasmids
TypeBlog Post..., Addgene then ships the material following our normal process. Due to recent regulation changes, an... -
PEI Calculator for Planning AAV Packaging Transfections
TypeBlog Post...AAVs AAVs Are Great Tools for Gene Expression in Mammals Resources on Addgene.org Find AAV Packaging and...