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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 850 results for: met

Showing: 561 - 580 of 850 results

  1. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...sequencing have prevented laboratories from using these methods for routine processes like plasmid validation. ... problematic for conventional NGS library prep methods because the yield or quality of individual libraries...

  2. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...Staining. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html Smith, A. & Hussey...medicine and more successful experiments. It’s something that all scientists should understand when stepping...

  4. Science Career Options

    Type
    Blog Post
    ...Sales Epidemiologist Scientific Recruitment Meteorologist Research and Development Healthcare Consulting...Lab Manager Patent Lawyer Technical Writer Meteorologist Journal Editor Textile Technologist/Designer...

  5. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...addressing these important questions that the scientific method cannot address. Listen to Our Podcast Interview...say that I'm glad we're so fast and affordable. Something in their world should be fast and affordable. ...

  6. How to Lead a Great Meeting

    Type
    Blog Post
    ... will too. Even if you are meeting to discuss something difficult, be excited about the prospect of working...meetings short, informative, and pleasant. Email: Sometimes, the right meeting is no meeting at all. When ...

  7. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...assignment that was open ended... it was actually something bordering on research, rather than 'here’s the..., sharing ethic that Addgene has behind it is something that very much resonates with me.” About Addgene...

  8. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...lab? Obviously, funding and support are crucial parameters, and familiarity with equipment always helps.... and dealing with email can be half of my day sometimes.” He points out that the copious amounts of email...

  10. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...Chemogenetics Chemogenetics offers noninvasive methods for manipulating cell activity, particularly in...targeting. While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases allow...

  11. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...cells/organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer plasmid...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...

  12. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...another factor, the receptor. The receptor could be something like an antibody, enzyme, DNA, etc. The receptors...One common biosensor class is designed to monitor metal ions. Within these ions, calcium is by far one of...

  13. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...deliver these systems virally instead of by another method? Viral delivery is attractive due to the ease of...viral vectors instead of a traditional transfection method. This is because viral systems have a high infection...

  14. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...conjugation reaction is done, through a variety of methods or commercial kits.  However, antibodies conjugated...January 31, 2022, from https://www.bu.edu/flow-cytometry/files/2013/06/Fluorochromes-Brightness-Chart.pdf...

  15. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...cell (National Research Council (US) Committee on Methods of Producing Monoclonal Antibodies, 1999). This...jbc.m116.736942  National Research Council (US) Committee on Methods of Producing Monoclonal Antibodies. Monoclonal ...

  16. How Addgene Creates Content

    Type
    Blog Post
    ...experts, watch webinars, or use other research methods. In the case of the antibody guide, primary research... as well as consults with microscopy and flow cytometry experts for the panel recommendations. A few discussions...

  17. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...Alternatively, you can simply make a note in your methods section about the edit that was temporarily used...a lot of space and don’t tell you why you did something or what the result was, so try not to do this....

  18. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ... from a polycistronic transcript. Although this method shortens construct size, it requires coexpression... be processed, a distinct advantage over other methods that favor certain nucleotides at the 5’ end. ...

  20. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...actually don’t have to worry about and that is something to recognize! Distributing plasmids, viral service...before it was cool or a necessary result of the #MeToo movement. We are carefully attentive to genuine ...
Showing: 561 - 580 of 850 results