We narrowed to 928 results for: mpl

...sequence used as an initiation point for PCR amplification or sequencing. Primers can be exploited for ...clone into. Regardless, once the cloning steps are complete, the vector containing the newly inserted gene...and troubleshooting tips to make plasmid usage as simple and straightforward as possible. If you have a ...

... or condition of interest using a simple keyword search. For example, searching the term "breast cancer...broadest possible scope of screens to be curated, for example phenotype screens with read-outs other than cell...

...and cell lines. Although ChIP-seq is a relatively simple and robust protocol it does have limitations. The...change offers a significant improvement and simplification of the original technique, specifically in ...CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids for isolating...

...Pinterest for scientists”? ReFigure extension is a simple Chrome plugin that allows you to save any image... even include your own data in a ReFigure. For example, you might include a confirmatory image to show...knowledge of which findings are reproducible. For an example, see our ReFigures on the therapeutic use of anti-CD47...

...this frequency can indicate behavioral state, for example, if someone is awake, agitated, or asleep. Though...performed in a live awake animal by surgical implantation of the probe into a specific brain region. While...neurotransmitters in various species and while exhibiting complex behaviors. Find dopamine and other biosensors in...

... a fast endophyte screening system and plans to employ the light‐inducible bacterial expression system...alternative source of essential omega-3 fatty acids complementing vegetable oil, a somewhat poor natural source...them. By using yeast integration vectors (see for example this yeast cloning kit) she intends to make yeast...

...given ion - we’ll use calcium biosensors as an example. Many calcium biosensors include GFP fused to calmodulin...reticulum (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted calcium...available, and the conditions of your experiment. For example, does the fluorescent tag need to be pH stable ...

...expressed. Once the design and construction steps are complete, it’s time to make virus! The constructed viral...the biological outcome you want to achieve? For example, do you want the nucleic acid cargo to be integrated... to achieve different biological outcomes. For example, some viruses integrate into the host genome (lentivirus...

... of origins of replication out there so, for simplicity’s sake, we've ignored those used in eukaryotic...manipulated with mutations in the replicon. For example, the pMB1 ori maintains about 20 copies per cell...sensitive; others ori's can be "tricked" into amplifying more copies with the addition of chloramphenicol...

...an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ... like as long as it’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas...also drive different levels of expression. For example, CAG is a strong promoter that drives very high...

...the public’s perception of head injury risk. In completing a master’s thesis in science communication, I... Department of Science Communication where he completed a master's degree in 2020, his research explores...athletes, as well as the ethical and health policy implications arising from sport-related concussion.   More...

...permanently attached to a protein, making them hard to amplify and image for long periods of time.  Are antibody... scientists fused them to sequences called complementarity determining regions (CDRs, or loops) of an ...demonstrated that the HA frankenbody can be used to amplify fluorescent signals in in vivo imaging: the 10xHA...

...phenotype typically emerges. Every three days, a sample of cells was collected for scRNA-seq analysis and...tended to have similar reprogramming outcomes. For example, cells with shared CellTagD13 tags often had similar...et al. "CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics." bioRxiv...

...jGCaMP7 sensors with different properties (for example, overall brightness or on/off kinetics) that makes...electrical activity in the brain was measured with implanted electrodes. The Archon1 voltage reporter allows...quantify targeting of particular cell types. For example, the mDlx enhancer restricts expression to GABAergic...

...in Columbus, Ohio, ABRC ships more than 100,000 samples to researchers and educators in 60 countries each...Engineering the Plant Genome Using CRISPR/Cas9 pSiM24: Simplifying Plant Genetic Engineering Resources on Addgene.org...Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory Lab Work MoClo Plants Parts...

...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ...dozens of other published FRET-based sensors. For example, his team is now applying it to improve upon an...monitoring calcium versus zinc should require the simple swapping of binding domains, in practice optimizing...

...agreement, read on!  What is the MTA?  An MTA is simply a contract or agreement that states the terms of...has placed a request for your plasmid, they must complete a Recipient Scientist Acknowledgment (RSA), which...use for the requested material. Once the RSA is completed, the recipient organization is sent the MTA to...

... you have something to ask or say. It can be as simple as “it was nice meeting you.” Include follow-up...person on LinkedIn I once helped by answering one simple question so I accepted the connection invitation...this because I want it to be clear that it is completely impossible for me or anyone to stay in touch ...

...lentivirus production frustrating - a researcher can complete an entire lentiviral production protocol only ...and we’ll be adding more as the need arises. For example, we’ve teamed up with the Broad Institute to provide...gRNA library in plasmid format, a researcher must amplify the library, use next-generation sequencing to ...

... It doesn’t have to be this way. We can never completely eliminate the risk that a minor variation in ...collective priority list. There has been progress—for example, a number of funders (e.g., the NIH) now look for...Press.  She trained originally in cell biology, completing her PhD at the MRC LMB in Cambridge UK and then...