We narrowed to 838 results for: tat
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TypeBlog Post...Society was founded in 1944 with the goal of facilitating networking between chemistry students, especially...over 170 members in its first year. The society facilitates opportunities to learn about Synthetic Biology...
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Sleeping Beauty Awakens for Genome Engineering
TypeBlog Post...interest allowing for insertion in the correct orientation. These plasmids use a novel synthetic RPBSA promoter... exciting tool for producing specific genetic mutations and gene disruptions without the use of viral ... -
Management for Scientists: Giving Feedback
TypeBlog Post...compare with your own perception? Tell me your interpretation of my feedback to you. What are We Learning...Steven Pinker. As a manager you must be able to state your observations truthfully and in clear terms.... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post...MarathonRT represents a major step forward in facilitating techniques that are dependent on long, error-free...sequence, fluorophore, or APEX2), allowing for quantitative analysis using different detection methods. ... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog Post...dissemination of unwarranted claims or unacceptable interpretations. A good peer-reviewer will help spot inconsistencies...data on ... The language is ... The general representation of data is... The figures are ... In general... -
Antibodies 101: Epitope Availability
TypeBlog Post...the protein folded, changing it from its native state to a less folded, or denatured, one. This enables... protein of interest while it’s in a transition state or specific configuration. So how do you ensure ... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...extract. Graphical Abstract: Schematic representation of cell-free system, capable of producing fluorescent...modify up to 60% of the disease-causing point mutations. They allow single-nucleotide modifications to... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post... to facilitate use. Dr. Trimmer’s lab will ensure the quality of the antibodies using quantitative assays... -
Antibodies 101: Epitope Tags
TypeBlog Post... This tag has been used successfully for immunoprecipitation, western blot, and immunofluorescence with...has the flexibility of also being used for immunoprecipitation, purification, and western blot as monoclonal... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...be examined. Increased yields with sucrose supplementation is serotype specific In our study we observed...preparations for, we wanted to be certain that this supplementation strategy would have no effect on the purity... -
Plasmids 101: TOPO Cloning
TypeBlog Post...cleaving and rejoining supercoiled DNA ends to facilitate replication. The TOPO technique specifically ...Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion and Deletions in... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post... the National Academy of Sciences of the United States of America 110.39 (2013): 15521–15529. PMC. PubMed... the National Academy of Sciences of the United States of America 70.11 (1973): 3240–3244. PubMed PMID... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post...requires very little hands-on labor and could facilitate the tagging of every protein in the C. elegans...other genome modifications, including targeted mutations. SEC-based systems may prove effective in other... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post...repository provides. Repositories around the world facilitate the rapid pace of SARS-CoV-2 research by removing...and SARS-CoV with implications for clinical manifestations, transmissibility, and laboratory studies of... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection. Expression is driven...QBI UCSF. They are particularly interested in facilitating collaborative science, and making it accessible... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...high contrast between its resting state and its calcium-bound state, which was achieved by lowering the... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post...cellular components ultimately leading to cell fragmentation and death (Elmore, 2007). To study apoptosis...fluorescence based reporters however have several limitations. First, FRET signals are often difficult to detect... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...makes it easier to spot even minor weakness or limitations in a piece of research (Drummond & Fischhoff,... the National Academy of Sciences of the United States of America, 114(36), 9587-9592. doi:10.1073/pnas... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...Society for Microbiology (2005). https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3/gram-stain-protocol... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...However, the first two generations of iGluSnFR had limitations: they were not optimal for experiments requiring...photon imaging. Figure 1: 1P and 2P excitation and emission spectra of SF-Venus-iGluSnFR.A184V...