We narrowed to 846 results for: tat
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TypeBlog Post...biosensors track cellular characteristics like pH, redox state, and voltage across membranes. These sensors generally... experimental setup? Consider what kind of instrumentation you have available, and the conditions of your...
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Bringing Sustainable Practices to the Lab: Recycling
TypeBlog Post...drop off the box at any location where the United States Postal Service picks up mail. NEB actually started...drop off the box at any location where the United States Postal Service picks up mail. Recycle plastic... -
Hot Plasmids - October 2022
TypeBlog Post...of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...a maximum absorption of 540 nm and two-photon excitation around 1,040 nm, has larger photocurrents, and... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post...disease typically caused by a single R206H point mutation on the receptor ACVR1. ACVR1 triggers appropriate...regeneration in healthy patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous... -
Networking and Inspiring Young Scientists at Freshers Fairs
TypeBlog Post...Society was founded in 1944 with the goal of facilitating networking between chemistry students, especially...over 170 members in its first year. The society facilitates opportunities to learn about Synthetic Biology... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog Post...interest allowing for insertion in the correct orientation. These plasmids use a novel synthetic RPBSA promoter... exciting tool for producing specific genetic mutations and gene disruptions without the use of viral ... -
Management for Scientists: Giving Feedback
TypeBlog Post...compare with your own perception? Tell me your interpretation of my feedback to you. What are We Learning...Steven Pinker. As a manager you must be able to state your observations truthfully and in clear terms.... -
Antibodies 101: Epitope Tags
TypeBlog Post... This tag has been used successfully for immunoprecipitation, western blot, and immunofluorescence with...has the flexibility of also being used for immunoprecipitation, purification, and western blot as monoclonal... -
Antibodies 101: Epitope Availability
TypeBlog Post...the protein folded, changing it from its native state to a less folded, or denatured, one. This enables... protein of interest while it’s in a transition state or specific configuration. So how do you ensure ... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...extract. Graphical Abstract: Schematic representation of cell-free system, capable of producing fluorescent...modify up to 60% of the disease-causing point mutations. They allow single-nucleotide modifications to... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post... to facilitate use. Dr. Trimmer’s lab will ensure the quality of the antibodies using quantitative assays... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post...MarathonRT represents a major step forward in facilitating techniques that are dependent on long, error-free...sequence, fluorophore, or APEX2), allowing for quantitative analysis using different detection methods. ... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...be examined. Increased yields with sucrose supplementation is serotype specific In our study we observed...preparations for, we wanted to be certain that this supplementation strategy would have no effect on the purity... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog Post...dissemination of unwarranted claims or unacceptable interpretations. A good peer-reviewer will help spot inconsistencies...data on ... The language is ... The general representation of data is... The figures are ... In general... -
Plasmids 101: TOPO Cloning
TypeBlog Post...cleaving and rejoining supercoiled DNA ends to facilitate replication. The TOPO technique specifically ...Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion and Deletions in... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post... the National Academy of Sciences of the United States of America 110.39 (2013): 15521–15529. PMC. PubMed... the National Academy of Sciences of the United States of America 70.11 (1973): 3240–3244. PubMed PMID... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post...requires very little hands-on labor and could facilitate the tagging of every protein in the C. elegans...other genome modifications, including targeted mutations. SEC-based systems may prove effective in other... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...under CC BY from Mattei et.al., 2022. Limitations of the in silico approach The in silico approach... verification. All approaches have their own limitations. Using methods together can inform decisions ... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post...repository provides. Repositories around the world facilitate the rapid pace of SARS-CoV-2 research by removing...and SARS-CoV with implications for clinical manifestations, transmissibility, and laboratory studies of... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection. Expression is driven...QBI UCSF. They are particularly interested in facilitating collaborative science, and making it accessible...