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Showing: 561 - 580 of 794 results

  1. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...error rate of current NGS approaches allows for a detection limit of roughly 0.1%. Thus, rare, pre-existing...prior to treatment, there are small (previously undetectable) populations of cancer cells that are resistant...

  2. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post
    ...control. But even with the advent of CRISPR/Cas9 technology, inserting large tags into a precise location...plasmid into the cells we use electroporation, a technique that briefly destabilizes the cell’s outer membrane...

  3. 6 Tips for Grant Writing

    Type
    Blog Post
    ...network of your peers. Furthermore, using new technologies to solve a problem can allow you to approach...different labs during your career; you learn new techniques and interact with new people. If you are looking...

  4. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...hexon proteins. The viral genome DNA is further protected by histone-like proteins forming the nucleocapsid... assays are commercially available for faster detection of RCA. 2nd Gen AdVs were designed to have increased...

  6. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...10.1021/acssynbio.9b00395 O’Brian, SP, et al., Biotechnol J.  2014. https://pubmed.ncbi.nlm.nih.gov/24390935...as time-lapse imaging or with high light power detection of molecules.  Lee, J. et al., Science Advances...

  8. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...to avoid mistakes due to miscommunication and to detect trouble. I’m not comfortable with awkward silences...someone more experienced to guide you - is valuable. Protect your team Make you team feel safe. Your team members...

  9. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...Yoshimi, K., & Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system...for genome engineering in human cells. Nature Biotechnology, 37(12), 1471–1477. https://doi.org/10.1038/...

  10. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...Lots of scientists work to invent novel techniques or to engineer improved tools for familiar applications...expressing good ol’ EGFP — showed a slight but detectable change in fluorescence when a small magnet was...

  12. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...physical models of proteins using 3D printing technology. The tagline for our company is “…where molecules...effective this model was in explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new...

  13. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post
    ...your plasmid prep We'll discuss the following techniques to increase plasmid yield. Keep in mind that ...that you might need to use multiple techniques for a particularly stubborn prep. Check the bacterial strain...

  14. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...expressing GFP from a given promoter, and the technique can be adapted to other transgenes. It’s important...dominance over HDR in this species. As CRISPR technology continues to develop, it’s become clear that ...

  15. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...advantage of CRISPR/Cas9 over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9...you'd like to brush up on the various CRISPR technologies, check out Addgene's CRISPR Guide. Figure...

  18. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature 538(7624) (2016):270-273. PubMed PMID...Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a/C2c2." Science (2017): eaam9321...

  19. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...best teachers and mentors pass on more than just technical knowledge, shaping their trainees’ very way of.... The MCC develops and makes freely available technical and pedagogical resources, offers mentoring for...
Showing: 561 - 580 of 794 results