We narrowed to 794 results for: tec

...hexon proteins. The viral genome DNA is further protected by histone-like proteins forming the nucleocapsid... assays are commercially available for faster detection of RCA. 2nd Gen AdVs were designed to have increased...

...control. But even with the advent of CRISPR/Cas9 technology, inserting large tags into a precise location...plasmid into the cells we use electroporation, a technique that briefly destabilizes the cell’s outer membrane...

...network of your peers. Furthermore, using new technologies to solve a problem can allow you to approach...different labs during your career; you learn new techniques and interact with new people. If you are looking...

...amounts of protein for any such disease. This technology therefore represents a potential platform that...CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and CRISPR resources on Addgene's...

...Lots of scientists work to invent novel techniques or to engineer improved tools for familiar applications...expressing good ol’ EGFP — showed a slight but detectable change in fluorescence when a small magnet was...

...Yoshimi, K., & Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system...for genome engineering in human cells. Nature Biotechnology, 37(12), 1471–1477. https://doi.org/10.1038/...

...to avoid mistakes due to miscommunication and to detect trouble. I’m not comfortable with awkward silences...someone more experienced to guide you - is valuable. Protect your team Make you team feel safe. Your team members...

...physical models of proteins using 3D printing technology. The tagline for our company is “…where molecules...effective this model was in explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new...

...your plasmid prep We'll discuss the following techniques to increase plasmid yield. Keep in mind that ...that you might need to use multiple techniques for a particularly stubborn prep. Check the bacterial strain...

...expressing GFP from a given promoter, and the technique can be adapted to other transgenes. It’s important...dominance over HDR in this species. As CRISPR technology continues to develop, it’s become clear that ...

...advantage of CRISPR/Cas9 over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9...you'd like to brush up on the various CRISPR technologies, check out Addgene's CRISPR Guide. Figure...

...the entirety of a cell. That's a big long-term technical challenge. We have a strategy that organizes how... into actionable signals. Let's say I wanted to detect every primary metabolite in a cell. The only way...

...CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature 538(7624) (2016):270-273. PubMed PMID...Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a/C2c2." Science (2017): eaam9321...

...best teachers and mentors pass on more than just technical knowledge, shaping their trainees’ very way of.... The MCC develops and makes freely available technical and pedagogical resources, offers mentoring for...

...knock-ins in mammalian cells. J Biotechnol., 16(4): 387-390 (2020). 10.1016/j.jbiotec.2019.11.010 Wright, D. W...

...them that their findings may be the result of a technical artifact that introduced an ancestry bias into... a reduction in the number of the dependencies detected. Given this new information, Misek notes that ...

...CRISPR-Cas9–mediated gene inactivation." Nature biotechnology 32.12 (2014): 1262. PubMed PMID: 25184501. PubMed... off-target effects of CRISPR-Cas9." Nature biotechnology 34.2 (2016): 184. PubMed PMID: 26780180. PubMed...

.... Not surprisingly, brain transduction was not detected. They also used an ELISA to measure binding of...widespread gene transfer to the adult brain." Nature biotechnology 34.2 (2016): 204. PubMed PMID: 26829320. PubMed...

...terms of the question, the methods, the tools or techniques that can help resolve the question, the anticipated... execute the proposal and the tools/resources/technology exist to solve the problem. What will you have...

...adeno-associated viral (AAV) vector toolbox. Biotechnology Journal 9:1402–1412 . https://doi.org/10.1002...in the mammalian brain using CRISPR-Cas9. Nat Biotechnol 33:102–106 . https://doi.org/10.1038/nbt.3055...