We narrowed to 819 results for: ust

...downright infuriating at worst. While it is tempting to just dive right in and start injecting your virus, a ...through the endogenous GFAP promoter results in robust transduction in the rat spinal cord following injury...

...study. These are highly useful for the biotech industry, which is in the process of capitalizing on phage...easily varied by the cell and whose structures are just beginning to be understood. Therefore, in addition...

...few gRNAs to choose from that sequence preferences must largely be ignored. Created with BioRender.com... For C>T and A>G base editors, the intended edit must be in a 5 - 10 nt window relative to the PAM, and...

...tools but their use is limited in vivo because they must be excited by external light sources. These external... subcellular structures and gene expression. You must see the movies demonstrating Ca2+ dynamics in cardiomyocytes...

...worked the first time probably because I used a robust cell line. If your cells are sensitive, it might...efficient at editing” to introduce the gRNA, but I just randomly chose one of my stable pools. Viruses containing...

... do so at a range of wavelengths, rather than at just one defined wavelength. Likewise, the emitted photons... spectrum shows two maxima, one local maximum at just under 500 nm and a global maximum at around 560 ...

... the sample are constantly exposed to light, not just those in the focal volume. This leads to more rapid.... Springer (2006). 7. Yang, Weijian, and Rafael Yuste. In vivo imaging of neural activity. Nature Methods...

...CBEs and ABEs are base transition editors. They adjust the side groups of DNA bases and leave the core...Protospacer-Adjacent Motif A short sequence that must be present in the non-target strand of DNA for Cas9...

... PubMed PMID: 22632979. 14. Sasaki, K., et al., Robust In Vitro Induction of Human Germ Cell Fate from...Central PMCID: PMC4169698. 24. Lian, X., et al., Robust cardiomyocyte differentiation from human pluripotent...

...with clinical endpoints. In the first scenario, robust and efficient generation of iPSCs is the number...Central PMCID: 5072705. 2. Sommer, Cesar A., and Gustavo Mostoslavsky. "The evolving field of induced pluripotency...

...blood brain barrier by endothelial transcytosis (Foust et al., 2009; Merkel et al., 2017), and these two...PMID: 19578477. PubMed Central PMCID: PMC2600441. Foust, K.D., Nurre, E., Montgomery, C.L., Hernandez, A...

...libraries, kits, and cloning grade DNA!     This is just the first of many milestones Addgene is poised to...

...Experiences (CUREs)Author: Jason PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors...

...to the blog to stay in the know!     No pranks, just fun and games this April Fool’s Day on the Addgene...

...Yit Heng Chooi Lab at the University of Western Australia.    🤩 our lab in Bayliss Building @SMS_UWA is...

...annoying. To make it easier, try this quick trick. Just flip, and press! Lab tip #2: Tube flick trick...

... GFAP promoter in rat. Contribute your AAV data Just like Addgene’s plasmid repository, the AAV Data ...

...these GIFs on Twitter, Instagram, TikTok and more. Just type “addgene” into the GIF search bar to find that...

... connected and collaborative community makes not just for a better day at the office but for stronger ...

...CRISPR Cheat Sheet here!   CRISPR Cheat Sheet Clustered Regularly Interspaced Short Palindromic Repeats...