We narrowed to 755 results for: ELP

...sharing-oriented, and there are many resources to help you learn more about fly biology and fly-based experimental...likely an easily accessible resource out there to help keep your research moving. Below, you’ll find some...tailored to your field of study. 5 great resources to help get your fly work started If you’ve never worked...hand corner. They have video tutorials under the help tab like this one to get you started. Any given ...Resources website has in vitro reagents and tools to help you knock down, knock out, and overexpress YFG in... are extremely effective. Their Snapdragon tool helps you select primers to synthesize RNA for gene silencing...

...offering advice on areas where you might need some help. Today in our transferable skills guide: managing...other teams, expanding your network…. I found it helpful to plan my week(s) in advance as much as possible...way to create more time for yourself, but it will help develop your team members by giving them new opportunities...good” or “fine” as an answer which is not really helpful. “What is the best/worst part of working with X...situation. But keep in mind that a good manager should help you work through your situation, not tell you what... former will empower you, keep you motivated and help you grow in your role. The latter will make you ...

...grad school, though often called “nontraditional,” helped prepare me to start CINSO. Here, I share some of...the tips I learned along the way and hope they’ll help you make the most of the opportunities you’re provided.... Framing my efforts in these comfortable terms helped guide me through the process. As I mentioned, my... time. Once, I had this crazy idea that I needed help with: wouldn’t it be fun to get several of the labs... organization. My short experience with my blog helped immensely when CINSO started brainstorming the ...a week to get recharged. The fun change of pace helped break up the negativity and allowed me to focus...

...Addgene Data Hub is here to help!  The Addgene Data Hub is designed to help scientists understand how our...are all in this together and everybody, helping everybody, helps everybody! References and resources ...Data Hub is a free resource to share this data and help speed scientific progress, but it will only be effective... effective if many scientists choose to help each other by sharing their data. So take advantage of it...

...transferable skills. At Addgene we're helping to fill this gap by both helping researchers recognize their MANY...offering advice on areas where you might need some help. Today, we’re highlighting an oft-underlooked transferable...in which people were asked to create a device to help students during an interview. When they were instructed...groupthink? Here are some ideas that I have found helpful in my team brainstorming sessions.  Allow the ...Lab Inventory Management system. I hope these tips help you to see that the creative skills you have developed...

...faculty members who can help you identify overly ambitious goals and can help you distinguish mechanistic...assays for your screen result may or may not actually help. Ultimately, if you cannot describe the likely outcome...question, the methods, the tools or techniques that can help resolve the question, the anticipated outcomes, ... Creating a coherent unified story and model can help make your ideas easier to understand and more exciting...length practices (~1 h) and get feedback. It’s also helpful to practice at least a few times with people that...

...papers with flow data... and to read them it's helpful to have done flow first… which can send you in ...to do on a plot generated in Biorender! While a helpful visualization tool, a quadrant will often be too...populations, I suggest using the imaginary quadrant to help (Figure 5). Even though this real-world data is ...messier than biorender plots, quadrants can still help me as a reader if I'm struggling to see the different...from Barlow-Anacker et al., 2017, with quadrants to help visualize the different populations. Used under ...would be good for your work. Hopefully this blog helps you with that…and happy flowing!    References ...

... step to any experiment and can help prevent future headaches. To help you plan PEI transfections for ... 1 Transfer Plasmid : 1 pHelper: 1 RepCap OR 3 Transfer Plasmid : 5 pHelper: 2 RepCap Select what type...based on using 28 μg of total plasmid (Transfer, helper, and RepCap added together) per 15 cm dish or 2500...

...names*, voted, and helped us on our arduous quest. Keep an eye out for Abi as they help us prepare for our...newest friend, our Addgene antibody mascot, here to help us with our upcoming affinity reagent distribution... best fits our new immunoglobulin friend? Please help us decide by voting for your favorite on this form...reagents. Name Our New Antibody Friend We need your help naming our latest mascot, an antibody joining us...

...mentoring to several people that found my insights helpful. All went on to get faculty positions at top state...success. What I can promise is that my suggestions can help you make the best case for your candidacy. A major...corresponding author publications in quality journals. It helps to come from the lab of a well-known investigator...guidance or the document examples are not especially helpful. I set out to make something practical and accessible...are not that group. If you read my eBook and it helps you, please introduce yourself to me by email or...compelled to repay me in some way, I encourage you to help your friends and colleagues with their job searches...

...years worth!) with academic scientists who can’t help us learn about the nonacademic sphere where most...job. Ask openly to be connected to someone who can help you land that position such as a direct connection..., use a written list and take notes if that is helpful. Keep in mind that this is a good time to ask personal...will get from the interaction. Most people like to help One good place to look for interesting connections...Benjamin Franklin Effect, most people actually enjoy helping (just ignore the people too busy to respond to ...

...excellent resume…it is worth it. Read here to get help if you are having trouble preparing your most excellent... you have a networking connection that will be helping (see below). Your resume must be in the official...small, any person at the company might be able to help you. If the organization is large, you will need...in your resume and don't be shy about asking for help. The worst thing that can happen is someone will...no, but if you don't ask, you will never get the help that can make a difference. Having your resume forwarded...

...experiment. However, by taking this time, you can help ensure that you understand the methodology and can...increase your rate of productive results and thereby help decrease the time to your first manuscripts. 4. ...decisions is complex, here are a few guidelines to help you evaluate projects:   If all of your experiments...create more detailed or functional models or more helpful background slides. If you don't have much new data... in empty calories will make you feel better and help maintain your health. Have a hobby- do something... a hobby that provides instant gratification can help boost your ego in between scientific achievements...

...raises money and awareness for rare disease and helps patients find the correct treatments, if available...are paired with patient partners so that they can help tell their stories. I was paired with a six-year-old...made her career as Senior Director of Biology, is helping us fight against FOP in two ways. First, Addgene...variety of research labs and hopes that these will help researchers build their knowledge of FOP, it’s biological... donation via the link below. Please continue to help bring HOPE to AJ.   PS: The superhero race is the...

...wrangling bacteria and instead help Addgene fulfill its mission of helping scientists share information ...put, I moved into this position because I want to help make the research enterprise work better and because...time when I was too nervous to ask store clerks for help at the mall. In undergrad I was forced to open up...These roles can include positions, like mine, that help support academia but the skills researchers gain...

...ensure the advisors they choose will be ones that help them succeed after the training period. While there...non-academic path, will the PI support this path and help you pursue any career alternative? Listen to our...encourage diversity? Knowing your own priorities will help in determining a good fit. Here are just a few suggestions...phone. Don’t use email to get information if you can help it. Observe as much as you can in person. Rotate...Additionally, if you are looking for more resources to help you choose a good mentor, I have provided a downloadable...

...we, as scientists, provide for journal editors to help decide whether work is suitable for publication ...unacceptable interpretations. A good peer-reviewer will help spot inconsistencies in the work, missing information... think that delivering constructive critique can help your colleagues at the receiving end, if not for...judge how aggressive my sentences might sound. What helps me in keeping the “right tone” is to address the...colleague in the office next door. So be kind, helpful, and thorough in reviewing the paper of your colleagues...

... to help a Mentee reach goals. One of the most important contributions made by a Mentor is to help the... any change in your skill sets or activate the helpful accountability that is one major advantage of working...careers, find a summer internship, etc.). It is helpful to work together on setting SMART goals using a...template. A Development Plan or mentoring journal will help a Mentee track progress, adjust expectations, and...

... guidelines to help authors understand what they are expected to do. All of that helps, but it’s only ...in an incubator), but there’s a lot you can do to help other scientists both what you did and build on ... citizen” and making sure you do what you can to help your fellow researchers advance science overall....open and willing to share with other researchers to help them troubleshoot and understand. All of this communication...

...the bioscience and tech transfer communities who help guide and promote Addgene in its mission to make... Institute and has worked with many Addgenies to help improve the understanding, curation, and explanation...recently sat down with Dr. Doench for an interview to help further introduce him to the Addgene community. ... was barely understood.” Doench’s graduate work helped expand our understanding of how miRNAs regulate...his work at the Broad Institute today, where he helps researchers in a variety of fields develop RNAi ...

...We have outlined some important considerations to help you choose the plasmid prep kit that is right for... significant source of waste in laboratories. To help combat this, some manufacturers have designed eco-friendly...for another time, however!) Where can you look for help?  All manufacturers provide specs, protocols, and...purification, reading the full instruction manual will help explain the rationale behind each step and provide...troubleshooting advice. In these cases, it can be helpful to select a kit with easy-to-follow instructions...reviewing their quality assurance documentation can help you select a kit. So...which kit should you pick...

...color controls are used to help you set up your voltages and can also be helpful for compensation. You should...isotypes can help you more easily gate your target population. In addition, the controls will help you save... flow cytometry, you’ll need various controls to help you set up and analyze your samples. While you are...

...SARS-CoV-2 plasmids to help academic and industrial labs accelerate research and to help find a faster solution... page on our website. Contributing your results helps other researchers using these plasmids and will ...have been instrumental in achieving our mission to help science move faster in the middle of a global pandemic... the reach of these plasmids is indeed global. Helping scientists speed up research is what we’re here...

...outline what works for me, and I hope that it is helpful to those of you just getting started. The practices...clearly stated, specific goal for each analysis will help you locate relevant information in the future. ...brief overview of the approach that you will take to help you plan your analysis. There is no need to go into...run. This technique leaves a trail that further helps you keep track of your exact changes. Alternatively...useful for those that are just starting out, and helps clarify the role of the code. # This is a comment...

...of work you put into it.” Have any other helpful tips to help transition a project from academia to a ...detect unwanted bacteria. How did graduate school help prepare you for the roles and skills necessary to...success in this business. A good presentation can help win a business plan competition and advance investors... its goals and vision for the company; this will help avoid future complications and potential conflicts...

...with Teytelman, Schwessinger joined this cause and helped by expanding this team, leading monthly calls, ...effort later in the year and in the fall of 2018, I helped organize a workshop for community scientists at...tracking material downloads. This was particularly helpful for securing funding to support this effort. The... the team has grown to include experts who could help provide new materials and reproducibility tools....April Clyburne-Sherin, Benjamin Schwessinger for helpful conversations on the history of the group, lessons...

...thinking “How can I help?” and not “What can you do for me?” One of the easiest ways to help is to connect ...build or use a network. Total strangers can’t really help or refer you. Do not ask people you don’t know for...people that want to meet or that you think might be helpful to each other. This is a quick way to do an easy...at that organization. This type of connection can help you gather information, make introductions, and ...

...analogues in nature. For example, helper plasmids were developed to replace helper viruses in AAV production ... entice their hosts to keep them around and even help them spread. They can be inherited by daughter cells...typically also carry the necessary resistance genes to help cells survive their own secretions. Pro tip! Historically...virulence plasmids! This category includes genes that help bacteria invade hosts, including attaching to host...

...collaborators can also help you get some critical insight into what you're doing wrong. All this helps to improve ...world for your research to rapidly progress. Need Help Building Your Scientific Network? Check out Our ...people to keep contributing to science, it would be helpful for them to know what we are doing and why. I always...grant for a proof-of-principal study were very helpful in assisting me with applications for much larger...

...be its own deep dive, but here are some general helpful principles (and I recommend Bustin, 2017 for a ...literature, a geNorm study (Vandesompele, 2002) will help you select the appropriate reference genes - and...reliable and reproducible data. Here are some tips to help you in your execution:  Accuracy is not as important...Setting up triplicates in multiple configurations can help you identify pipetting or evaporation errors. And...occur. A passive reference dye, such as ROX, can help normalize for that. Passive reference dyes are inert...automatically record fluorescent data on all channels - helpful if you forget to set the channel wavelength before...

...useful. We’ll need something like Google Maps to help us identify and visualize addresses. That’s where...where enhancers and genome math come in. They help us to transform raw genomic coordinates into meaningful...difference. That’s the intuition for how genome math helps us to locate interesting addresses in the genome...powerful stretches of DNA known as enhancers with the help of genome math. Figure 1: Genome math illustrated...yet to explore. I hope that this brief guide can help you do just that. Good luck, and always keep going...

...listed below can give you the resources and path to help you enter and to have a successful and meaningful...passion for the arts? Do you find satisfaction in helping your local food shelf raise money? Do you have ...consultant, building your area of expertise will help you find work and target your professional development...and coaching services, including Smartegrants, to help those entering the Grants Profession. Whether you...first career, a mentor in the Grants Profession can help you focus your career goals, identify your niche...

...offering advice on areas where you might need some help. Today in our transferable skills guide: Collaborating...to respond in a supportive way and are willing to help because you are not (yet) asking for a job or a ...outside my university approachable, friendly, and helpful. Finally, don’t forget about collaborations and...land you a job someday. Universities themselves can help you do this through practical tools like alumni ...organizations in science and beyond. For example, I help organize scientific workshops, manage conference...

...the hopes that these findings can help others as much as they've helped us.  Interested in providing feedback... to ensure that the service has grown steadily, helped by a partnership with the University of Pennsylvania...broadest category of viral vector tools, which may help explain why they are the most commonly requested...Browsing the Data Hub before selecting an AAV can help a researcher make a more informed choice on which...

...Understanding the function of these genes in fish will help clarify their roles in humans. The sequenced genome...Additional analysis tools have been developed to help parse and visualize expression data in useful ways...mutagenesis screens. Microscopy-based lineage tracing can help illuminate how signaling pathways—such as those ...cell types. Sequencing-based lineage tracing can help elucidate the gene networks that transform zygotes...this field and others are likely to continue with help from zebrafish. Many thanks to our guest blogger...

...vocabulary for this skill. What this post may do is help you realize whether or not you have a natural inclination...resolve conflicts within a given organization, it can help tremendously to have as much context as possible... conflict resolution? Definitely not. But it can help, and if and when you find yourself transferring ...you are able to broker a compromise well, you can help everyone walk away from a conflict happy, feeling... the workplace is inevitable, but having who can help resolve these conflicts is not inevitable. When ...

...added bonus to knowing your audience is that it can help you avoid making mistakes. I once made a joke about...doing it for the first time? Practice talks also help a speaker determine how long the talk will really...clear and concise answers. This preparation will help you avoid giving only yes or no answers in front...ready after your formal conclusion slide will also help with your confidence. It is best not to be asked...the sharing model works. See if you can elicit helpful comments from your audience as in “Does anyone ...

... a tremendously positive experience. I enjoyed helping people and learning to explain concepts in ways...from my role in Industry and took evening work to help cover the costs. My MSc courses were interesting...my idea of a dream job, but I knew that I enjoy helping people and decided to compromise and applied anyway...job. Because of my background in genetics, I can help scientists problem solve experiments over the phone...email me are scientists who are grateful for some help.  I also get the chance to contribute content to...

...obstacles; for instance there are few resources to help get individuals started on the path to internationalization...talented professionals to their original countries to help build, together with the universities, a bright ...level where I would like to be in a few years, helping me to advance in my career.” Adrian Mancebo (Mentee...discussion topics at IMP In these sessions, mentors helped mentees create “Career Development Plans” which...goals, and identified possible funding sources to help them carry out their plans. Through their shared...

...listed? Help us serve you! Let us know about other organisms we should include by emailing us at help@addgene.org...editing techniques in your microbe of choice. For more help with CRISPR, read our recent blog post explaining...narrow your keyword search to one of these organisms. Help us continue to develop resources for your community...