We narrowed to 163 results for: NAP

1. Newly Updated AAV Data Hub!

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   ...instance, this report shows that anterograde, monosynaptic connections between BLA projection neurons and...

2. Fueled by Coffee at #SfN14

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   ...dent in the global coffee supply… My back-of-the-napkin calculations put the daily consumption here at ...

3. Supporting Rare Disease Research with BeHeard 2017

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   ...the Institute of Biosciences and Bioresources in Naples, Italy. His group works on spinal muscular atrophy...

4. Working 9-5: an Addgene Co-op

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   ...labs intended. With the use of applications like SnapGene and Geneious, I was able to determine if any discrepancies...

5. Year of the Camelid: Antibody Style

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   ...maintaining a llama facility is out of reach and unappealing to many labs), the field is moving towards using...

6. Running for Rare Disease, Running for FOP, Running for AJ

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   ...antibody that binds Activin A and interferes with inappropriate activin-ACVR1 interactions prevents FOP bone...

7. Sensing Neuronal Dopamine

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   ...Dopamine-sensitive adenyl cyclase: possible role in synaptic transmission." Science 174.4016 (1971): 1346-1349...

8. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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   ...proteins and tags including mCherry, HaloTag and SNAP-tag for multi-color imaging. Find the HA frankenbody...

9. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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   ...very high, ubiquitous expression, while the human synapsin 1 promoter is neuronal specific (Haery et al.,...

10. New and Upcoming Viral Vectors - December 2019

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    ... Plasmid Serotype Name 106174  AAVrg pAAV.hSynapsin.SF-iGluSnFR.A184S Viral vectors coming soon...

11. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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    ...constructs with containing chemical tags such as the SNAP-tag, Halo-tag, and CLIP-tag (Kohl et al., 2014) ...

12. 10 Great Guest Posts We're Thankful For

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    ...artefacts in your experiments. Read this post from Erik Snapp, Associate Professor at Albert Einstein College ...

13. Visualizing Translation at the Single Molecule Level

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    ...biochemical method developed in 2009, takes a “snapshot” of all of the mRNA bound-ribosomes in a cell....

14. Revamp Your Lab Meetings With Creative Virtual Collaboration

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    ...miscommunication, a lack of leadership, and inappropriate tools or writing approaches can lead to frustration...

15. Celebrate National Book Month with Addgene

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    ...(which had been lost for an age) fight in the Napoleonic Wars and against something even more sinister...

16. Networking Follow-up: I Met a Person. Now What?

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    ...Networking connections can last years This is a graphic snapshot of my LinkedIn network from a few years ago using...

17. The Importance of High Titer for AAV Transductions

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    ...Zingg, Brian, et al. "AAV-mediated anterograde transsynaptic tagging: mapping corticocollicular input-defined...

18. Fluorescent Proteins 101: Luciferases

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    ... (luciferin),730–743 (analogs) 64 Luciferin or naphthyl-luciferin analogs ATP No Deep sea copepod (Gaussia...

19. Viral Vectors 101: The AAV Retrograde Serotype

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    ... neurons and six hundred trillion connections (synapses.)  In this post we’ll describe the AAV retrograde...

20. Sharing is Caring: But How to Distribute Open Hardware?

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    ...optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling, et. al, "Robotic microscopy for...

21. A Deep Dive into BioBuilder

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    ...and in more than 30 countries around the world. Snapshots of the BioBuilder experience With its intentionally...

22. Plasmids 101: Methylation and Restriction Enzymes

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    ...illustrates how sequence visualization software (we used Snapgene here) can help you assess whether your restriction...

23. Why and how to start studying chromatin (with Addgene's help)

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    ...grows on a daily basis, the lists below provide a snapshot of relevant resources and a good jumping off point...

24. Antibodies 101: ChIP

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    ...ChIP experiment! Image courtesy of https://www.wannapik.com/   Even though ChIP requires a larger ...

25. Hot Plasmids - February 2022

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    ...anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag fusions, while the anti-HA frankenbodies...

26. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    ...base editor corrects early stop codons within T7 RNAP. A phage encoding the deaminase TadA-7.10 A mutagenesis...

27. Plasmids 101: Screens vs. Selections

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    ...lot in common. In fact, selections are often inappropriately categorized as screens! Why the confusion? ...

28. RANbodies: Reporter Nanobody Fusions

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    ... GFP, RFP) Detecting GFP reconstitution across synaptic partners (GRASP) assays No antibody required...

29. Seven Tips for Using LinkedIn as a Scientist

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    ...updates, or click bait of any kind. For more on inappropriate content see this article. However, do comment...

30. RNA Extraction Without A Kit

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    ...These products, namely RNAlater®  from Thermo and RNAProtect® from Qiagen, are ammonium sulfate-based solutions...

31. Addgene's Tips for Plasmid Quality Control

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    ...examples include Benchling, Serial Cloner, and Snapgene), and save these to a shared computer or server...

32. Fluorescent Proteins 101: Introduction to FRET

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    ...spider silk, or the activity of a protein in a synapse. What kinds of tools enable us to measure these...

33. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...selectable markers by detecting common features using Snapgene. If we find information that differs from what...

34. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    ...and Nancy Kleckner. "Recombination, pairing, and synapsis of homologs during meiosis." Cold Spring Harbor...

35. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    ...for the visualization of endogenous proteins and synapses. Feng S et al. Commun Biol. 2019 Bo Huang Near-Infrared...

36. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    ...neurons, and are especially abundant in neuronal synapses. shRNA silencing of one circular RNA they have...

37. Degrading DNA with Cascade-Cas3

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    ...Wright, J. T., & Taylor, D. W. (2023). Structural snapshots of R-loop formation by a type I-C CRISPR Cascade...

38. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP) tags, a selectable marker (floxed Cbr-unc-119) ...

39. AAV Vector Quality Control: Going the Extra Mile with NGS

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    ...The images in this blog post were created using Snapgene and Geneious software. References and resources...

40. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ...Drosophila cell lines are extremely effective. Their Snapdragon tool helps you select primers to synthesize RNA...