...three plasmids: (1) The transfer plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These...lentiviral vector will infect, pseudotyping can help. In pseudotyping, you produce viral vectors using viral...Sandoval S (2017) Pseudotyped Lentiviral Vectors: One Vector, Many Guises. Human Gene Therapy Methods ...To deliver genes using lentiviral vectors, you need an envelope protein on the virus’s surface and a ...virus. How to pseudotype To pseudotype your viral vector, you use the same protocol for generating lentivirus...pseudotyped protein is encoded on the envelope plasmid which contains a promoter, the envelope gene, and...Sometimes this is due to the cells that the viral vectors were produced in. For example, production of vesicular...

...functionality. We wanted users to be able to find plasmids and viral vector tools more easily. In the redesign, the...every time you come to Addgene to find the plasmid or viral vector for your next experiment, that you can ...look clean and easy to use.  Easily search for plasmids (and more!) The main goal for the homepage was...on the homepage, Blugene is now accompanied by an AAV friend.  We encourage you to take a look and explore...

...are transfected with both vectors. Flex vectors are the solution. Flex vectors expressing fluorescent cytoplasmic....org Find Cre-Lox Plasmids Find Optogenetics Plasmids Find Chemogenetics Plasmids .... It became possible to design FLEx vectors (Flip-excision vectors) that only allow one inversion event...networks Double inverted vectors expressing light inducible ion channels (plasmid # 20298 for example) have...concentrations of vectors expressing the required recombinase. The high concentration of FLEx vectors guarantees...Learn More about FLEX-vectors Learn More about Cre-Lox Check out Retrograde AAVs for Neurscience Research...experiments. Mutated FRT and loxP sites in Flex Vectors – inversion only once All recombination events ...

...cost required to make a plasmid. In 2023 (and at closer to 2 million plasmids shared than not!), we think...great Plasmid Debate of ’18, Blue Flame depositor John Doench calculated the time to make a plasmid. Doench...insertion of a PCR product into a vector. The time it takes to make a plasmid could increase dramatically if... make a plasmid? Doench also calculated the cost of consumable reagents used to make a plasmid, which ...which came out to $115/plasmid, excluding any restriction enzymes or vector purchases (Table 1). Restriction... to make plasmids that already exist, and we significantly reduce costs of producing plasmids with high-throughput...lot of plasmids, it’s almost always faster to order from us!) Ask the PI If you want a plasmid for free...

... Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website, Data...is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists to ...on the Addgene Blog Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

... viral service Find optogenetics AAVs for your research Find all AAVs from the viral service ...neurons that could be activated with light.  Find plasmids from the Isacoff lab! They then tested what kind...optogenetics blog posts Read blog posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org...

...toolbox. Natural plasmids, also called environmental plasmids, are all around us! A plasmid is a small piece...colicin-producing plasmids have been referred to as “Col plasmids” or “Col factors.” Virulence plasmids: microbial...based on these vectors (Shareck et al., 2004). Plasmid categorization is complex Plasmid classification...Old Solution but a New Problem Plasmids 101: What is a Plasmid? Plasmids 101: Transformation, Transduction...F. (2023). Plasmid classifications. Plasmid, 126, 102684. https://doi.org/10.1016/j.plasmid.2023.102684...., 2001) Beneficial traits of natural plasmids Natural plasmids often convey a variety of functions that...functions of natural plasmids. Created with BioRender.com. Resistance (R) plasmids: microbial defense...

...double crossover event between the vector plasmid and the packaging plasmid caused the Psi sequence to be ...generation of lentiviral vectors consisted of three plasmids encoding 1) the lentiviral vector genome which was... the transgene   Transfer plasmids The transfer plasmid is the vector you clone your gene of interest...your experiment using the lentiviral vector system. Viral vectors have been increasingly popular in fundamental...primary cells. Amongst the different vectors used, lentiviral vector constructs have proven very useful ...fluorescent sensors. Retroviral vector background Until the development of viral vectors, gene transfer into mammalian...un-transfectable cells, labs use viral vectors and particularly retroviral vectors because they target a broader...

...transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral plasmid containing the cargo...Adenoviral Vector Production and Troubleshooting Additional resources on Addgene.org AAV Production...T-antigen. This allows them to replicate viral plasmids with the SV40 origin of replication. To generate...Platform to Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol. (2021) (9):796991. DOI...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Human whole genome...cells with sgRNAiBAR library containing lentiviral vectors the iBARs can be detected by next generation sequencing...manipulations such as injection of precise titrations of AAV or heat-shocking the organism. The SPARC system uses...4X CDRE repeat. In addition to showing that this vector is suitable for flow cytometry and fluorescence...attached fluorescent protein to the DNA. They created plasmids for purification of tTALE-eGFP and tTALE-mCherry...

...Addgene.org Browse Our Viral Vector Plasmids Read Our Lentiviral Vector Guide Read Our AAV Guide  ...encoded on separate plasmids and are co-transfected into cells alongside a plasmid encoding the minigenome...four viral genes in the same plasmid (as opposed to four separate plasmids), there are a few reasons arguing...separate (and thus smaller) plasmids. Finally, having four separate plasmids allows researchers to partially... 2 μg minigenome plasmid per well (in a 6 well plate format). However, since plasmid quality and transfection...producing infectious virus (similar to lentiviral vectors) because they do not encode all of the necessary...Minigenome System). From a more technical standpoint, a plasmid with all four genes and necessary control elements...

...researchers developed adeno-associated viral (AAV) vectors for the targeted delivery of a functional MECP2...mouse model using an adeno-associated viral (AAV) vector. The editase repaired half of the mutant MECP2...their MECP2-related plasmid tools with Addgene and share additional resources. Plasmid tools to facilitate...to partner with them to launch a Rett Syndrome Plasmid Collection and Resource Center for Rett Syndrome... Rett Syndrome in novel ways.  Addgene’s MECP2 Plasmid Collection and Resource Center will continue to...

...on the Addgene blog New to viral vectors? Browse our Viral Vectors 101 blog posts Read blog posts about...targeting expression involve tissue specific promoters, AAV serotypes, and the ever-growing variety of recombinase...viral preps for your research Find chemogenetics plasmids for your research ...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern CH, ...

...arrays, which is scalable and efficient in plasmid and viral vectors. In applications when repetitive tRNA ...number of gRNAs that can be packaged into viral vectors for potential in vivo applications or multiplex...elements for deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing...unprecedented number of genes with a single viral vector.   Fig. 1: Schematic of multiplex gene ...manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715) and hCtRNA_VT (#186716).   ...

...Download our Plasmids 101 eBook to get all the background you need to start working with plasmids! What are...Techniques Read all of our Plasmids 101 Posts Resources on Addgene.org Browse our Plasmid Reference Pages Just...Plasmids designed to express genes in a given host cell type are generally broken down into two broad...based on the functional elements they contain. Plasmid DNA in both prokaryotic and eukaryotic systems ...Rho-dependent terminators are not usually employed in plasmid-based expression systems, so these will not be ...the end.  Nearly all common bacterial expression plasmids use Rho-independent terminators, which include...terminator will suffice. Many commercial expression vectors use double terminators to reduce unwanted translation...

.... Viral vectors undergo DNA rearrangements. Depending on the composition of the viral vector and the ...about Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages ... one point or another. After amplifying a viral vector, collecting virus, and transducing target cells...the repeat sequences present in the LTR, the vector may undergo genomic rearrangements during replication...genomic rearrangements, try amplifying the viral vector with bacteria designed to minimize rearrangements...to their transduction has been shown to increase AAV transduction efficiency by approximately 15-fold ...have low titers (which is unavoidable for some vectors) you can concentrate your virus by ultracentrifugation...

...small capacity vectors like AAV. In addition to the mammalian option described below, plasmids for making ...multiple gRNAs on the same plasmid, you’ll make sure that each cell that gets the plasmid contains all of the...into distinct expression vectors using the enzyme BbsI. These expression vectors each contain Type IIS restriction...rectangles) are cloned into various plasmids using oligonucleotides. These plasmids contain Type IIS restriction...different purposes.   Gersbach Lab multiplexing plasmids: This plasmid set allows you to express 2-4 gRNAs, with...Cas9 or dCas9-containing destination vectors. Destination vector options include humanized wt Cas9, dCas9...transcriptional activator)-containing plasmids. Each destination vector contains GFP, enabling you to select...

...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles. ...than SpyCas9, allowing them to be packaged in AAV vectors, potentially with multiplexed targets.   ...be used for applications beyond cryo-EM.   Find plasmids for MagIC-Cryo-EM here!   Arimura, Y., Konishi...disease phenotype in an animal model. Find PE-eVLP plasmids here! The lab’s other recent deposit builds on...over the wild-type enzyme. Find optimized PASSIGE plasmids here!     Figure 3: Schematic of the PASSIGE...recombinase integrates a sequence from a donor plasmid containing a complementary site into the genome...from Neumann et al. 2024. The team showed that AAV-delivered CHARM could silence the expression of prion...

...Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that will not be published are...and entering a plasmid name,plasmid type, and plasmid description/experimental purpose (right). On the...with your plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given...Feng Zhang (Addgene plasmid #52961)".   The vector type should describe how your plasmid is intended to be...lentiviral, retroviral, and AAV plasmids, we recommend the NEB Stable strain. For plasmids containing the ccdB...easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with...Spreadsheet". Start your plasmid deposit There are two options to begin your plasmid deposit. Figure 2: Choose...

...you’re working with small capacity viral vectors such as AAV. Base removal RNA-guided CRISPR enzymes cause...for short. Addgene has already distributed this plasmid all over the world, and the question on everyone...identified NgAgo and showed that it can cleave plasmid and genomic DNA in a variety of mammalian cell ...homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and... contrast, RNA guides must be expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary.... Epub 2016 May 2. PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan...24531762. PubMed Central PMCID: PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, ...

...Lentiviral Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids... that made it easy to share plasmids, both for people depositing plasmids and for people requesting them... Verify Your Plasmid Using a Restriction Digest Analysis Videos What is a Plasmid? How to Design...video, however, is “What is a Plasmid?”, An animated explainer of a plasmid and its parts that helps the...them. Prior to this, many plasmids were available, but they often weren’t easily accessible, due to a ...barriers, allowing people to access a wide catalog of plasmids from all over the world. This idea, that available...Views Guides CRISPR Guide Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus Guide ...

...move antibiotic resistance genes between plasmids or from plasmids to the bacteria’s genome. In humans, if...which is a big advantage over viral vectors (~5 kB payload for AAV and ~8 kB payload for lentivirus). ...in viral vectors, but easily fit in a TE. Transposons are also less likely than viral vectors to induce...transposon’s LTRs, similar to when packaging viral vectors.   Let’s take a closer look at three different ... in human cells at rates similar to retroviral vectors. The hyperactive version of the SB transposase,...activity (Voigt et al., 2016). Sleeping Beauty plasmids  piggyBac Although its name suggests otherwise...efficiency in human cells similar to retroviral vectors. The hyperactive PB transposase (hyPB) is ~10-fold...

...Would you buy and use a plasmid if it didn’t include any plasmid sequence data? Of course not. Unfortunately...depositing your plasmids encoding antibodies, ScFvs, and nanobodies to Addgene! The plasmids will be made...distributions of research materials, such as plasmids and viral vectors. However, it typically takes a few years...Plückthun, 2015a, b). Recombinant antibodies are plasmid-based and offer numerous advantages, including ...antibodies that you have come to expect from our plasmid and viral services. The first step will be working...working with the community to build a resource of plasmids encoding recombinant antibodies as well as other...crowdsourced Data Hub (built off of the concept of our AAV Data Hub) to share further validation data with you...

...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...et al., 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent versions ...ONE vector G-protein Optical (ONE-GO) biosensors (Janicot and Maziarz et al., 2024). Each plasmid expresses...selectivity. With 10 plasmids available as a kit, plus 21 optional accessory plasmids, the collection targets...precise plant breeding. Find dicot prime editing plasmids here! Vu, T. V., Nguyen, N. T., Kim, J., Song... proteins for mammalian cell expression. These plasmids will be useful not only for future studies of ...proteins of interest too! Find CLIP, CLAP, and SNAP plasmids here! Smith, E.R., Turner, E.D., Abdelhamid, ...

...stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized, it’s important...for future preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections...culture, but Ran et al. have shown that CRISPR and AAV can be used together for postnatal genome editing...facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR does not cause obvious germline modification... Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your...with AAV9, whereas Nelson et al. and Tabebordbar et al. used the shorter SaCas9 with AAV8 and AAV9, respectively...

...deliver in low-capacity vectors, such as adeno-associated viral (AAV) vectors. In 2017, Zetsche et al....Francisella Cpf1 (FnCpf1), they employed an E. coli plasmid depletion assay to discover FnCpf1’s PAM sequence...system." Cell (2015). PubMed PMID: 26422227. Find plasmids from this publication at Addgene. 2. Zetsche,....1 (2016): 31-34. PubMed PMID: 27918548. Find plasmids from this publication at Addgene. 3. Kim, Daesik...Biotechnol. (2016). PubMed PMID: 27272384 Find plasmids from this publication at Addgene. Kleinstiver...Biotechnol. (2016). PubMed PMID: 27347757. Find plasmids from this publication at Addgene. Makarova, Kira...PMID: 26411297. Resources at Addgene Find CRISPR plasmids at Addgene Read Addgene's CRISPR Resource Guide...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'...Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system is a cost-effective alternative... ladders. Image from Santilli, et al. 2021 Plasmids are used to express 10, 15, 20, 30, 40, 50, 60...50 mLs) or through two polycistronic expression vectors (3570 lanes/50 mLs) coexpressing a set of either...kD proteins or 20, 40, 60, and 80 kD proteins. Plasmids containing 150 or 250 kD proteins are also available...

... BRAIN initiative plasmid collection More resources on the Addgene blog Plasmid tools for microbiome... of a live animal. They started by delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a...studies Channel rhodopsins for optogenetics Viral Vectors 101: Chemogenetics ...

...acids) means they can be delivered in vivo using AAV vectors. Having a way to shut off CRISPR-Cas systems ...Papayannopoulou T, Lieber A (2018) HDAd5/35++ Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR...CRISPR   Resources on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible...light-inducible Cas9 activity? Check out these these plasmids Catch Up on Your CRISPR Background with Our Guide Pages...

...size also allows for their delivery by viral vectors like AAV, which could be useful for delivering scFvs...insect cells, they are most often expressed from a plasmid in bacteria (Ahmed et al., 2012). One challenge...domains of the selected scFvs are inserted into a vector encoding the antibody constant domain scaffold ...doi.org/10.1038/nbt.1791  Deal CE, Balazs AB (2015) Vectored antibody gene delivery for the prevention or treatment...Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies for neuroscience research...

...or GONAD (Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9 either through... Find CRISPR Plasmids for use in Mammals Check out Our CRISPR Guide Pages Find Plasmids for Mouse Targeting...the target site, and a donor oligonucleotide or plasmid as the repair material if needed (for knock in ..., the gRNA, Cas9, and donor oligonucleotide or plasmid components are brought together and microinjected...provided (in the form of an oligonucleotide or plasmid), precise changes can be made in the genome via...mice carrying the intended mutation. With a donor plasmid, the outcome is less predictable. In our best case...Mediated Genome Editing with Adeno-Associated Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci...

...ddPCR) for lentivirus vector titration.... Protocols ddPCR Titration of Lentivirus Vectors ddPCR Titration of...of Lentivirus Vectors You may also like... Lentivirus Colony Formation Titration Assay Lentivirus Fluorescence...Fluorescence Titration Assay AAV Titration by qPCR Addgene Viral Service Lentivirus Guide Virus Blog Posts...droplet digital PCR (ddPCR) to titer lentivirus vectors. This protocol specifically uses primers and probes...green) and negative (black) droplets Sample ID Plasmid ID Dilution Copies/20 µL RRE (FAM) Copies/20 µL...

...some permanent knock out plasmids? Check out our Cre vector and Murine LoxP vector.   Turn it off for a little... a gene (cDNA) can be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted for...? Check out our Retro expression vector and Lenti expression vector. Turn it on for just a little while...out Addgene's Lentiviral dCas9 SAM vector and Piggybac dCas9 SAM vector! On/off control all the time If ...Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging vector! Destabilizing domains Destabilizing...resources on the Addgene blog Plasmid 101: Inducible Promoters Plasmid 101: Repressible Promoters CRISPR...Addgene's Lentiviral Tet-on and AAVS1 targeted Tet-on transgene vector. Degron tags Tagging relevant proteins...

...101-Viral Vector Elements Blog: AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids AAV Plasmids... Virus (AAV) Plasmids Additional Resources: AAV Guide Biosafety Guide All Viral Plasmids Blog: Plasmids...our AAV Guide for more information about: AAV components Common uses of AAV Viral integration AAV serotypes...Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6 All Helper plasmid for AAV packaging...Available AAV Packaging Plasmids This table contains helper plasmids for packaging your AAV product. You...table based on plasmid name, capsid, and more. ID Plasmid Gene/Insert Mutations PI AAV Plasmids This table...AAV2 AAV packaging plasmid, expressing Rep2 and Cap2 Melina Fan 104964 pAAV2/5 AAV5 AAV packaging plasmid...

...dependent viral vectors. Jonathan Ting 172907-AAV1 pGP-AAV-syn-FLEX-Volton2-ST-WPRE AAV-mediated expression...Addgene's AAV are made from a selected set of plasmids that are currently in our repository. AAV are distributed...Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven KOR ...Edward Boyden 108422-AAV5 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 AAV production plasmid encoding for Archon1...119742-AAV5 AAV SYN PSAM4 GlyR IRES EGFP Chemogenetics expression plasmid Scott Sternson 119744-AAV5 AAV ...element Jordane Dimidschstein 135630-AAV9 pAAV-S5E2-dTom-nlsdTom AAV vector to drive the expression of dTomato...element Jordane Dimidschstein 135631-AAV1 pAAV-S5E2-GFP-fGFP AAV vector to drive the expression of fGFP in...

...suffix (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description ... Viral Vector Packaging Service AAV Serotype Testing AAV Viral Vector... Vector Packaging Service: Serotype Testing AAV These AAV encode fluorescent reporters and can be used...Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made from plasmid 37825 (...Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made from plasmid 50465 (...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our... Add to Cart AAV Retrograde 37825-AAVrg.T 20 µL $ 140 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description...

...Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC Cell type-biased: Astrocytes - AAV-PHP.AX with GRE ...Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP.... Viral Vector Packaging Service AAV Caltech Systemic Capsids Viral Vector Packaging Service...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...and NHPs: Broad tropism - AAV-MaCPNS2, AAV-PHP.S Sensory nervous system - AAV-MaCPNS1 Return to top...the pUCmini-iCAP-AAV.MaCPNS1 plasmid (Addgene #185136) . Browse Available MaCPNS1 AAV ID Name Promoter...the pUCmini-iCAP-AAV.MaCPNS2 plasmid (Addgene #185137) . Browse Available MaCPNS2 AAV ID Name Promoter...

...Viral Vector Packaging Service AAV Retrograde AAV Viral Vector Packaging Service: Retrograde AAV As part...filtering options. Browse all Retrograde AAV *The AAV retro helper plasmid was generated as a part of the Janelia...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...laboratories. These viral vector preparations were produced with the rAAV2-retro helper plasmid (Addgene #81070)...viral production , these viral vectors undergo quality control, including AAV titering by SYBR green qPCR...catalog items. Browse Available Retrograde AAV See our retrograde AAV based on the functional catagories listed...Promoter Activity Category PI 37825 AAV-CAG-GFP CAG GFP Control Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP...

...cloned into a vector containing Cas9 and the PITCh-gRNA. Step 3: Contransfect the PITCh vector with the vector...Guide Pages Find CRISPR Plasmids for Your Experiments Deposit Your New Plasmid Tools with Us  ...repair, making it easier to construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed...Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell lines can be created in about... knock-ins, some labs have used NHEJ for whole plasmid integration; however, since NHEJ is error-prone...just upstream of a stop codon. Briefly, the PITCh vector should be constructed with 5’ and 3’ microhomology...cloning. An overview of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and ...

...Zebrafish Tobin 135618 pAAV-Sox2-CreERT2 Cre-ERT2 - Tamoxifen inducible; AAV donor vector AAV He 135659 pQcre1... Plasmid Collections Cre-lox Plasmids Cre-lox Plasmids Additional...Cre-lox Plasmids Addgene's Blog: Cre-lox Addgene's Blog: FLEx Vectors Cre-containing Plasmids loxP Constructs...107312 AAV-hSyn-mCherry-P2A-Cre-WPRE mCherry and Cre; expressed in neurons hSyn AAV Yang 107313 AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA...Deisseroth 55635 pAAV-EF1a-sCre SCre EF-1 alpha AAV Deisseroth 55636 pAAV-EF1a-Cre Cre EF-1 alpha AAV Deisseroth...CamKII AAV Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Cre hSyn AAV Wilson 105555 pENN.AAV.hSyn.Cre.hGH Cre ...51507 AAV pmSyn1-EBFP-Cre Cre-EBFP fusion; Expression in neurons. synapsin AAV Zeng 51904 paavCAG-iCre ...

...Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre...Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse the following tables for in-stock AAV preps...provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of site...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...See our Cre-lox Plasmids page for more information about these molecular tools. Cre AAV ID Name Promoter... rg* Wilson 24593 AAV-pgk-Cre PGK none rg* Aebischer 51507 AAV pmSyn1-EBFP-Cre Syn EBFP 5 Zeng 105540 ...50363 AAV phSyn1(S)-DreO-bGHpA Syn none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI ...

...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...using our virus protocols . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral... and quality control are described below. AAV Production AAV distributed by Addgene has been produced ... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core (Link ...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....determine which facility produced your viral vector lot. At Addgene, AAV particles are titered by droplet digital...

...technologies. Penn Vector Core AAV Vectors Many AAV vectors from the Penn Vector Core have been transferred to Addgene...eligible AAV plasmids and we’ll make the viral vector prep you need. Packaged on Request Viral Vector Packaging... Vector Packaging Service Addgene produces high-quality viral vector preps from select plasmids in our...service for plasmids and pooled libraries. Viral preparation options include lentivirus and AAV. Viral.... We know that viral vector production is a common step after requesting plasmids from Addgene, and by...In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock preps. These high-titer preps...and ready-to-ship AAV or lentivirus prep for your next experiment today! Browse AAV | Browse Lenti Packaged...

... Viral Vector Packaging Service AAV Browse In-Stock AAV Adeno-associated virus (AAV) vectors are safe...control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 8, 9 and retrograde Retrograde AAV To target...in-stock AAV preps are affordable, available in small volumes, and ready to ship. All viral vector preps ...need, try Addgene's AAV Packaged on Request service. You can choose a transfer plasmid from our repository...All Serotypes If an AAV is available in serotypes AAV1, AAV2, AAV5, AAV8, AAV9, AAVrg, the Caltech systemic...serotypes (AAV1, AAV2, AAV5, AAV8, or AAVrg). For more information, visit our Viral Vector Packaging Service...-use adeno-associated virus (AAV) available from Addgene's viral vectory packaging service. Browse constructs...

... Results Eligible Plasmids Additional Resources Adeno-associated virus (AAV) vectors are safe and useful...paper of your chosen AAV plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter...Discover our AAV Packaged on Request service with all-in-one pricing for viral vector preps, which includes... Viral Vector Packaging Service Packaged on Request AAV Packaged on Request Overview...using AAV Packaged on Request, scientists can start experiments as soon as they receive their AAV prep ...transfer plasmids in our repository are eligible for this service. Select any eligible plasmid in the Addgene...transfer plasmid, including legal approval and an implementation letter DNA amplification Viral vector production...

...Gradinaru 164450-PHPeB CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain...CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA DLX2.0 (3xCore version of DLX enhancer). AAV vector for strong... BRAIN Armamentarium Project below. Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description...-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq...Ting Viviana Gradinaru 213945-PHPeB pAAV_BiSSTe4_ChR2_mCherry AAV construct expressing mCherry-tagged ...Fishell Viviana Gradinaru 213944-PHPeB pAAV_BiSSTe4_dTomato_nlsdTomato AAV construct expressing dTomato driven...Fishell Viviana Gradinaru 213941-PHPeB pAAV_BiPVe3_ChR2_mCherry AAV construct expressing mCherry-tagged ...

...in vivo either by electroporation of plasmids, or using AAV vectors. The Imai lab also developed a version...a specific tissue. XFPs are introduced using a plasmid construct called Zebrabow-GateDest containing an...

... we would recommend using a cDNA overexpression vector rather than going through all the troubleshooting...et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences from a given... CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on Addgene.org Find Cas9 Activators Find...

...: Viral Service: All AAV Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part of our Viral ...viral vector preparation. These viral vector preparations were produced with the pTR-UF11 plasmid ( Addgene...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...parental AAV44.9 serotype. It was developed by Shannon Boye’s lab. Plasmid Information The plasmid below ...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida... Viral Vector Packaging Service AAV University of Florida Serotype Testing...viral production , these viral vectors undergo quality control, including AAV titration by ddPCR, in vitro...

... Viral Vector Packaging Service AAV Monosynaptic Neuronal Tracing Viral Vector Packaging Service...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G... Tracing These AAV encode tools for rabies virus-based monosynaptic tracing. These AAV can be used with...steps for using AAV and modified rabies virus (RABV) for monoclonal neuronal tracing. (1) AAV encoding TVA...deletion-mutant rabies virus AAV1 Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used...Ready-to-use AAV available from Addgene's viral service encoding tools for monosynpatic retrograde neuronal...