Plasmids 101: Biotinylation
Type
Blog Post
... Sciences, 24.9 (1999b): 359–363. PubMed PMID: 10470036.
3. Said, H. M. “Biotin: The forgotten vitamin...
We narrowed to 75 results for: 104
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Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog Post.... PubMed PMID: 9537413. PubMed Central PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog Post...Regulation. Plant Physiol 169:971–985 . https://doi.org/10.1104/pp.15.00636 Ma X, Zhang X, Liu H, Li Z (2020) Highly... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post...Chem 295:926–939 . https://doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser J, Stark GR (1979) Transfer... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post...368. https://doi.org/10.1146/annurev.genet.40.110405.090448 Kumar A (2020) Jump around: transposons in... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...Self-Excising Drug Selection Cassette. Genetics 200:1035–1049 . https://doi.org/10.1534/genetics.115.178335 Dokshin... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post.... Plant Physiol 178:989–1001 . https://doi.org/10.1104/pp.18.00828 Additional resource on the Addgene ... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...Reports, 3(4–6), 64–72. https://doi.org/10.1007/s41048-017-0037-8 Lucidi, M., Capecchi, G., Visaggio,... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...nanoscopy." Nature biotechnology 26.9 (2008): 1035-1040. PubMed PMID:18724362. 8.Stiel, Andre C., et al.... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...understand the Earth! Denver, CO, USA 39.7392°N 104.9903°W - Erin Golden Hi, my name is Erin and I have... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...Cell Stem Cell, 2010. 7(1): p. 15-9. PubMed PMID: 20621044. PubMed Central PMCID: PMC2913590. 12. Zhou, T... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...Steinfeld et al., ACS Chem Biol 2014 May 16;9(5):1104-12. Heinemann et al., FEBS Lett 2012 Oct 19;586... -
Retrograde AAV viral preps
TypeCollection...GENIE Project 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn GCaMP7s Calcium sensor Douglas Kim 104489 pGP-AAV-syn-jGCaMP7b-WPRE...Douglas Kim 104488 pGP-AAV-syn-jGCaMP7f-WPRE Syn GCaMP7f Calcium sensor Douglas Kim 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE...pAAV-hDlx-Flex-dTomato-Fishell_7 Dlx dTomato Control Gordon Fishell 104055 pAAV-CAG-eYFP CAG EYFP Control Viviana Gradinaru...pGP-AAV-syn-jGCaMP7b-WPRE Syn GCaMP7b Calcium sensor Douglas Kim 104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn GCaMP7s, Cre-dependent...Cre-dependent Calcium sensor Douglas Kim 104493 pGP-AAV-syn-FLEX-jGCaMP7b-WPRE Syn GCaMP7b, Cre-dependent...Cre-dependent Calcium sensor Douglas Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG GCaMP7s, Cre-dependent Calcium...Calcium sensor Douglas Kim 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG GCaMP7f, Cre-dependent Calcium sensor... -
Caltech Systemic Capsids
TypeCollection...mRuby2 Control Gradinaru 104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP..., GENIE 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn Calcium sensor GCaMP Kim , GENIE 104488 pGP-AAV-syn-jGCaMP7f-WPRE...element Activator DREADD Dimidschstein Biosensors 104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor...sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn Calcium sensor, Cre-dependent...Cre-dependent GCaMP Kim , GENIE 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP...GCaMP Kim , GENIE 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , ...PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP CAG EYFP, Cre-dependent Control... -
Immunology Research Plasmids and Resources
TypeCollection... HBD-3, HBD3, HBP-3, HBP3 DEFB104A defensin, beta 104A BD-4, DEFB-4, DEFB104, DEFB4, MGC118942, MGC118944... mu DKFZp686I15196, DKFZp686I15212, FLJ00385, MGC104996, MGC52291, MU, VH IGHV@ immunoglobulin heavy variable... inositol polyphosphate-5-phosphatase, 145kDa MGC104855, MGC142140, MGC142142, SHIP, SHIP1, SIP-145, hp51CN...like 1 464.2, D17S1718, G0S19-2, LD78, LD78BETA, MGC104178, MGC12815, MGC182017, MIP1AP, SCYA3L, SCYA3L1 ...-C motif) ligand 4 ACT2, AT744.1, G-26, LAG1, MGC104418, MGC126025, MGC126026, MIP-1-beta, MIP1B, MIP1B1...CKRX, CRAM-A, CRAM-B, FLJ55815, HCR, MGC116710, MGC34104 CKLF chemokine-like factor C32, CKLF1, CKLF2, ... -
Biosensor AAV Preps
TypeCollection...GCaMP7b 104489 pGP-AAV-syn-jGCaMP7b-WPRE Syn jGCaMP7b none Constitutive 1, rg* Douglas Kim 104493 pGP-AAV-syn-FLEX-jGCaMP7b-WPRE...jGCaMP8m none Constitutive 1, 9, rg* Loren Looger 210400 PRS_8-GCaMP8m PRS jGCaMP8m none Constitutive 2 ...2 Simon Wiegert Calcium Sensor: GCaMP7f 104488 pGP-AAV-syn-jGCaMP7f-WPRE Syn jGCaMP7f none Constitutive...Constitutive 1, 8, 9, rg*, PHPeB Douglas Kim 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn jGCaMP7f none Cre dependent...dependent 1, 8, 9, rg*, PHPeB Douglas Kim 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG jGCaMP7f none Cre dependent... rg*, PHPeB Douglas Kim Calcium Sensor: GCaMP7s 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn jGCaMP7s none Constitutive...Constitutive 1, 9, rg*, PHPeB Douglas Kim 104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn jGCaMP7s none Cre dependent... -
Control AAV Preps
TypeCollection...Gradinaru 100896 pAAV.GFA104.PI.eGFP.WPRE.bGH GFA104 EGFP Constitutive 5 Philip Haydon 104055 pAAV-CAG-eYFP...Constitutive 5 Hongkui Zeng 58909 pAAV-GFAP104-mCherry GFAP104 mCherry Constitutive 5 Edward Boyden 59133...Constitutive 2, 5, rg*, PHP.eB Viviana Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Viviana...pAAV-CAG-FLEX-EGFP CAG EGFP Cre dependent 1 Ian Wickersham 104052 pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent PHP.V1... -
The Pleiades Promoter Project
TypeCollection... Ple103 HAP1 pEMS1367 EGFP/NLS Ple104 HAP1 pEMS1084 EGFP/cre/NLS Ple104 HAP1 pEMS1368 EGFP/NLS Ple105 ... Ple135 NR2E1 pEMS1043 cre/EGFP/NLS Ple135 NR2E1 pEMS1351 EGFP/NLS Ple136 NR2E1 pEMS1044 cre/EGFP/NLS ...NLS Ple227 THY1 pEMS1048 cre/EGFP/NLS Ple227 THY1 pEMS1419 EGFP/NLS Ple228 THY1 pEMS1049 cre/EGFP/NLS Ple228...Ple137 NR2E1 pEMS1609 intron-lacZ/NLS Ple138 NR2E1 pEMS1046 cre/EGFP/NLS Ple139 NR2E1 pEMS1611 intron-lacZ... -
Fluorescent Proteins: FRET
TypeCollection....49 527 104,000 0.64 5.3 2.3 mCerulean N1 , mVenus N1 mTurquoise2 mVenus 434 0.93 527 104,000 0.64 5.9... 435 0.51 530 104,000 0.77 5.3 2.3 CYPet-N1 , YPet-N1 mTFP1 mVenus 462 0.85 527 104,000 0.64 6.0 2.9 mTFP1...mNeonGreen-mRuby2-FRET-10 SYFP2 mScarlet3 515 0.68 592 104,000 0.75 6.3 4.8 pSYFP2-C1 , pmScarlet3_C1 , pDx_mScarlet3...pLSSmOrange-mKate2 LSSmOrange* mScarlet3 437 0.45 592 104,000 0.75 5.8 4.3 pLSSmOrange-N1 , pmScarlet3_C1 mT-... -
Neurodegeneration Plasmid Collection
TypeCollection...Parkinson's Sachdev Sidhu 104448 pEGFPC1-hVAP-B VAPB GFP, HA CMV ALS Catherine Tomasetto 104450 pEGFPC1-hVAP-B KD...Tomasetto 104465 hnRNPA2_LC_P298L HNRNPA2B1 His T7 Multisystem proteinopathy, ALS Nicolas Fawzi 104466 MBP-... ALS Nicolas Fawzi 104478 TDP-43_NTD1-80_Y4R TARDBP His T7 ALS Nicolas Fawzi 104479 TDP-43_NTD1-80_E17R...T7 ALS Nicolas Fawzi 104480 pJ4M/TDP-43 TARDBP His, MBP T7 ALS Nicolas Fawzi 104481 pJ4M/TDP-43 S48E TARDBP...'s Dan Mulvihill 110486 Ultra-Exon1Q23-Myc-A HTT Myc UbC Huntington's Baoji Xu 110487 Ultra-Exon1Q145-...Huntington's Baoji Xu 110488 pUltra-Exon1Q23-Myc-B HTT Myc UbC Huntington's Baoji Xu 110489 pUltra-Exon1Q145...GFP GPD Huntington's Susan Lindquist 15569 GPD 104QDProGFP p416 HTT GFP GPD Huntington's Susan Lindquist...