We narrowed to 200 results for: ADAP
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TypeBlog Post...recombinase-based state machines and how they function. Adapting Toehold Switches to Detect Zika Virus The RNA-... understanding of Cas9 biophysics! Casilio: An Adaptive, Multitasking “CRISPR-OS” In this post, Albert...
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Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post...researcher. There existed, therefore, a need for an adaptable tool that could continuously monitor multiple ...- Overview of the neural and gut fiber system (adapted from Sahasrabudhe et al., 2023). To develop... -
What the HEK?
TypeBlog Post...adherent monolayer, though derivatives have been adapted for growth in suspension. Of the many derivatives... allows for large scalability, and can also be adapted for serum-free growth, which significantly reduces... -
25 Work From Home Tips for Scientists
TypeBlog Post...career paths and things you can do to prepare. Adapt to WFH while parenting Keep your kids on a schedule... your work schedule as possible so that you can adapt to your kids' needs. Plan ahead. Think about and... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...Nature Communications 2019. Pubmed PMID: 31366908 Adapting sigma54-dependent transcription for CRISPR activation...must be σ54-dependent and the system may not be adaptable to distantly related species from E. coli. This... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog Post...RNA-seq was performed to map TFs to cell fates. (Adapted from Joung et al.) This atlas linked TF ... color coded nominated reference cell types. (Adapted from Joung et al.) Putting MORF to work ... -
Hot Plasmids - January 2023
TypeBlog Post...nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from Figure 3 Colognori et al 2022. Find CRISPR...temperatures, and in the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods ... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...high school classrooms, but it could be widely adapted and used in a variety of settings. If you are a..., if you are a fellow researcher interested in adapting your research ideas into hands-on educational ... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...their devices, which makes it very difficult to adapt the device to one's own samples and limits the use...to as 'frugal science', existing components are adapted and combined with creativity and feedback from ... -
What's New in CRISPR - November 2021
TypeBlog Post...packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA sequences to instead drive...delivering gene editing tools into cells. Image adapted from Segel et al. 2021. Prime editing improvements... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...features and evolved PAM sites, making Cas9 an adaptable choice for many genome engineering endeavors. ... Choosing a Cas While Cas9 may be the most adaptable Cas currently available, the other Cas proteins... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...for assembling the barcoded gRNA library pool. (Adapted from Wong et al., 2016). With a list of gRNA...assembling barcoded combinatorial gRNA libraries. (Adapted from Wong et al., 2016). With CombiGEM and ... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post...in optogenetics, opening up the possibility of adapting GFP lines for optogenetics experiments in specific...transcription activation domains. Both systems are easily adaptable to neuroscience applications, including optogenetics... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...few steps. More recently, CRISPR technology has adapted Golden Gate cloning for inserting the appropriate...single gRNA-expressing plasmid, but it can also be adapted to express multiple gRNAs. Addgene has two Golden... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester 2021. Alternative ...Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science. 2012;337(6096):816... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post... the simplest CRISPR systems and the easiest to adapt to genome editing, but the introduction of type ...positions are indicated below each graph. Figure adapted from Kleinstiver et al., 2016. Until recently... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce...for using CRISPRi in many types of bacteria: lab-adapted, environmental isolated, and disease-associated... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post...section below.) For guide design, we decided to adapt the popular guide design tool Crispor by Max Haeussler...Crispresso CrispRVariants AmpliCan We chose to adapt Crispresso by Luca Pinello and Kendell Clement for... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post...MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic tools, they’re optimistic...optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools. Embracing science... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post...spreading within and between genomes, and have been adapted for a wide variety of applications - as mutagenesis...at two different incubation temperatures. Data adapted from Vo et al., 2020. In E. coli, the new streamlined...