...Andrew Leifer’s lab utilized the worm to study calcium signaling in the entire adult brain, using a plasmid...plasmid that expresses a genetically encoded calcium sensor, GCaMP6 (Nguyen et al., 2015). If you are ...PMC5349099. Nguyen, Jeffrey P., et al. "Whole-brain calcium imaging with cellular resolution in freely behaving...

...it to create a standard curve on your blot to calculate the amount in your sample. (Do ensure your blot.../Reprobe PVDF membrane Increased time Calculate absolute quantification Running a standard curve...wells; purified protein not always available Calculate relative quantification Normalizing to ubiquitously...

... CRISPR-based method for cell lineage tracing Valcyrie is a CRISPR-based marker excision method for marking...target. Read the paper in Development Find the Valcyrie plasmids If you have a new CRISPR tool you’ve...

... lot of their plastic packaging (from Corning®, Falcon®, or Axygen® product packaging) for free! It has... If you use serological pipettes from Corning®, Falcon®, or Axygen®, set up a bin or box in your tissue...

...-encoded reporters of molecular signals; e.g., calcium indicators In this overview, we will focus on the... truncated forms of cytosolic STIM1 to control calcium channels ~488 LOV domains LOV-based Light-oxygen-voltage-sensing...light-controlled molecules. ~450–495 BACCS light-activated calcium channel switch iLID Improved light-inducible dimers...

...manipulation in the lab. This is commonly done using calcium chloride which permeabilizes the cell membrane ...and physical methods in the lab. Chemicals like calcium phosphate and diethylaminoehtyl (DEAE)-dextra neutralize...

...will image these neurons using GCaMP7, measuring calcium signaling related fluorescence during morphine ...enable multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure...

...the transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of ...be optimized prior to starting. We recommend calculating the ratio of the surface area of the larger vessel...

...to your normal control - you can set up your calculations so that your assay output number (RQ value) ... determination of amplification of Plasmodium falciparum multidrug resistance-1 gene (pfmdr1). J Parasitol...

...vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...terminal providing better signal to noise for imaging calcium dynamics in fine structures. We have expanded our...

...qPCR Protocol for more details. Pro Tips When calculating the titer for your AAV, remember to account for...tool for gene expression in mammals Use the PEI calculator for planning AAV packaging transfections Find...

... plate Fidelity of assembly (percent correct) Calculated colony totals Per 2 μl assembly reaction Per...outgrowth volumes described in Figure 3, with calculated yields from entire outgrowth built from 2 µl ...

... commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin... of how bright is the emission. Brightness is calculated as the product of extinction coefficient and ...

...also need to know the extinction coefficient to calculate the concentration. At 280 nm, the extinction coefficient...protein standard and the concentration may be calculated using imaging software. Any bands on the gel ...

...-55 °C for the homologous region (calculated using Oligo Calculator). Insertions - To insert short sequences...

... and ways to navigate it, we’ll search for the calcium biosensor, GCaMP, and click through to the plasmid...distribution. If your experiment involves delivering a calcium biosensor using AAV, you might decide you’d really...

... ligands (Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category ... vectors available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization...

...level of antigen present in the sample and is calculated by extrapolating from the standard curve. Because...amount of antigen present in the sample and is calculated by extrapolating from the standard curve. Scientists...

...Used for neuronal/CNS expression. Modulated by calcium and calmodulin. GAL1, 10 General expression mRNA...ADH1 General expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length...

...value cut off. This method entails arranging all calculated p values from smallest to largest and ranking...ranking them (1 being the smallest value). It calculates the cut off for significance by finding the largest...