We narrowed to 949 results for: App;

...pre-synaptic mGRASP-mCerulean for synaptic connectivity mapping. 5 Jinhyun Kim 34912 paavCAG-post-mGRASP-2A-dTomato...post-synaptic mGRASP-dTomato for synaptic connectivity mapping. 5 Jinhyun Kim Tools for Cell Ablation These AAV...

... any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200...

...particles can be identified after negative staining and appear darker than full vector particles. Figure 3: Electron...replaced with a new vial of early passage cells. Approximately 2 weeks post-thaw, cell culture supernatant ...

...factor, erythroid 2 like 2 NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 PAK...their downstream targets. Rajalingam K, Schreck R, Rapp UR, Albert S. Biochim Biophys Acta. 2007 Aug;1773...

... Spike protein RBD mutations for comprehensive mapping. SARS-CoV-2 Spike Receptor Binding Domain Site-... Spike protein RBD mutations for comprehensive mapping. Other Resources Protocols Coronavirus Method Development...

...use. Since 2006, scientists have developed many approaches to generate iPSCs. The generation of iPSCs is...Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014...

...amino acids (ncAA) in a protein of interest. Applications By making small changes in selected amino acids...Davis L. & Chin J. W. (2012). Designer proteins: applications of genetic code expansion in cell biology . ...

...sure I missed some of your favorite apps. If you know of a software app that I missed, let me know in the... The software can be used with the ZappyLabs Bench Tools mobile app (available for iOS and Android). A...system does not appear to have a way to share your protocols with other groups. What apps do you use to ...publishers, and small start-ups are turning to software apps to solve our need for quicker, more effective updating...more to fit every lab's needs. Plus there are the apps - many to help you access your protocols at the ...comments below and leave your thoughts on how the app works for your lab’s needs. At Addgene, we’ll continue... some of our basic protocols to the protocols.io app so that all of our researchers can easily take those...

...previous nosy question! The Application Process Broadly speaking, the PhD application process tends to go something...steps in the application process and usually occurs in the few months leading up to applications opening. ...program. Most applications will recommend that you name two professors in your application that you intend...to continue the application process through a campus visit, a weekend where applications can get to know...strengthen your application and help you decide if a program is a good fit for you. The application process looks...if you’re anything like me, the process of even applying to one of these programs is almost as daunting...be, at times, very overwhelming. As someone who applied to PhD programs in Biology around the United States...

...technique published every week! One of the newest applications is a colorful system that uses fluorescently...Thoru Pederson’s lab, brings us one step closer to mapping the 4D nucleome, the organization of the nucleus...activity, catalytically dead Cas9 (dCas9) has many applications, the most well-known being transcriptional activation... 9 and 13. They next turned their attention to mapping pairs of intrachromosomal loci. The technique successfully...authors’ knowledge, this work represents the first mapping of intrachromosomal loci, a major benchmark in ...characterizing the 4D nucleome. Future modifications and applications Ma et al. developed this technique using standard...for this technique. The NIH Common Fund has made mapping the 4D nucleome a specific priority, as researchers...

...exhaustive list of all the apps out there, we encourage you to mention any apps you’ve found useful in the... other posts that deserve a second look. 5 Great Apps for Lab Life This very useful post was contributed...provides a brief rundown of Sean’s top five smartphone apps you can use in the lab. Whether you’re tired of ...that chemical in your obscure growth medium, these apps can help make your lab life a bit easier. Since ...

...their use in other clinical applications. In the last decade, viral applications in cancer treatment and ...s unpack the myriad number of emerging viral applications and give you some recommendations for putting...putting viruses to work. Viral applications Viruses work by infecting a host cell (the target of viral infection...expressed. It’s important to note that for all the applications we discuss, there is more than one type of virus...retrovirus, AAV, etc.). When you’ve decided on an application, our Viruses as Biological Tools blog can help...viruses and pick one for your experiment. Research applications Gene expression control Researchers are always...becoming increasingly popular tools with many applications. These libraries include sgRNAs targeting the...

...have been developed for different scientific applications. These PCR methods all use the same basic PCR...quantified by qPCR. qPCR is used in a variety of applications including gene expression profiling, studying...that fractions the PCR reaction sample into approximately 20,000 droplets. Each droplet contains the material...

... typically occurring with a fluorescence-based approach. These challenges can include background autofluorescence...real time. Let's take a closer look at several applications of this technology. In each section below, we... attempts to purify or overexpress proteins inappropriate in discerning their true roles. Work conducted...context-dependent activity can render traditional tagging approaches insufficient. Vascular endothelial growth factor...

...function (Wood, et al). In the microbial world, approximately 50% of predicted genes have unidentified roles...endogenous gene. Each trap uses a slightly different approach (described in detail below), but fundamentally...redundant genes – multiple genes which function in overlapping roles can mask the depletion of one another. ...trap systems lack a promoter and thus require the trapping of an endogenous promoter. The system is composed...enhancer traps lack enhancer elements and require the trapping of endogenous enhancers to stimulate the expression...many more! Check out Addgene’s catalog to find trapping tools for your next experiment! References and...

...and applications.    Plant applications We outlined many synthetic biology MoClo applications, but...for synthetic biology applications in bacteria or cell-free systems. The approach is particularly useful...- and multi-gene constructs for a variety of applications. MoClo tools can be used in bacterial and cell-free...and the kits Addgene offers to kickstart these applications, no matter what your model organism is. MoClo...this blog, we will highlight some common MoClo applications and kits. Keep in mind that this technology ... it, MoClo might help you achieve it! MoClo applications and specialized tools Synthetic biology in bacteria...effectiveness. Perhaps the most intuitive CRISPR MoClo application is to assemble multiple unique sgRNAs into a ...

...options, you’re likely to find an application that matches your needs. Happy quantifying!  References and...blog post, we're going to focus on PCR-based applications (qPCR and ddPCR.) qPCR Quantitative/real-time...range of RQ values as noted above.   In both applications, results can be affected by DNA quality and ...as positive or negative for each reaction, math happens (this time, Poisson statistics), and the readout... triplicates. ddPCR is an extremely accurate application, even in areas of the genome that are difficult...experiments, a number of factors will determine which approach is right for your experiment. You’ll have to consider...QuantificationPolymerase Chain Reaction Overview and Applications Resources on Addgene.org Protocol: Polymerase...

...particular application can be found here.  Why CRISPRa? Although cDNA overexpression approaches may still... viral vectors, and high-throughput screening approaches. Here, we’ll highlight these recent advancements...advancements and offer commentary on their application to neuroscience research. What is CRISPR-based activation... a larger packaging capacity than AAV. Such an approach was described recently by the Day Lab, who developed...accessible to neuroscientists, not all neuroscience applications require such optimized tools. This is elegantly... developed a subgenome-scale CRISPRa screening approach in embryonic stem (ES) cells to determine which...study neuronal differentiation, it could also be applied to the differentiation of other CNS cell types,...

...The Framework for In vivo T cell Screens (FITS) approach (Milling and Markson et al., 2024), developed ...compatible with single gene KO and pooled screening approaches; (4) encodes a Thy1.1 selectable marker to facilitate... et al., 2024.   Proof of concept The FITS approach was used to optimize conditions for a screen that...