We narrowed to 569 results for: BLI

...circular assemblies. While Golden Gate Assembly worked for all the assembly types, circular assemblies yielded...J., & Lohman, G. J. S. (2020). Enabling one-pot Golden Gate assemblies of unprecedented complexity using... an increase in the complexity. In 2018, they published a paper profiling four base overhang ligation ... researchers design high-fidelity Golden Gate Assemblies and overhang sets: NEBridge Ligase Fidelity Viewer...Figure 1: Workflow for high complexity Golden Gate Assemblies. Image courtesy of New England Biolabs.   ...the 52-piece reaction shows massively complex assemblies are possible, the two sets of data together indicate...

... One obvious way to do that is and has been to publish in journals with a high “impact factor,” a measure... citations for recent articles. Of course, a publication in Science or Nature is always nice, but in the... lesser-known journal as it is in a top-tier publication. Accessibility is surely also behind the increase...increase in citations for older publications. (As an interesting aside, Nature recently reported that the...in a solution.) But are citations and journal publications really the best way to measure impact? As that...and what it’s made of. Increasingly, journal publications alone aren’t enough. Scientists are sharing ...consider and quantify the impact of scientific publications. Ventures like Impact Story seek to find ways...

...2013 there have been 33 CRISPR software tools published and documented by OMICtools. It must be confusing... are altogether different. They are filled with blind anticipation. Their trust in the quality of the ...reviewing the contents of the piñata and the audience blindly receiving them; it results in highly similar content...; it is caused by not having the time to assess public response; and, it is the stage I think we are currently... CRISPR software submissions. With few prior publications to go on, a submitted manuscript could only ...The first of only three such databases, EENdb (published 1 Jan 2013, no longer available), is a simple ...sgRNA designs. The second database, CrisprGE (published 27 Jun 2015), is noteworthy for its broad scope...

...for Microbiology (ASM) Scientific Writing and Publishing Institute. Writing is the cornerstone of any ... them with the world. If one doesn’t write and publish in some way, then the data produced isn’t likely...simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts and...and the ASM Scientific Writing and Publishing Institute (SWPI) is an extremely beneficial guide to becoming...field of microbiology with multiple high-profile publications. My classmates consisted of graduate students...paper will be generated. After you complete a publication quality figure, produce the figure, its legend...Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific Manuscript Writing Scientific Manuscripts...

...Multiple first or corresponding author publications (published, not in preparation) A history of successful...A project with sufficient preliminary data to establish your own lab and get an independent investigator...the beginning of Year 5. Now work backwards. To publish a submitted manuscript in a high-profile journal...The other items will follow. If you get more publications, you will be more competitive for fellowships...typically have 4-5 years to make discoveries and publish papers to position yourself for a job search. Therefore...the best possible outcome, e.g. where could you publish this and how important is the result? Is there... meet future collaborators and get access to unpublished information and reagents. Part of the reason ...

...isolation. It published a paper a year (or less) and limited the exchange of information to the public and to...to other academic labs. Being unable to publish or attend conferences makes it very challenging to compete...As a Master’s student, she had less pressure to publish and more freedom to attend conferences, but after...occasionally thinking about how we could bypass the establishment hiring system altogether. We had a lot of ideas... organisms, and GMOs have a bad reputation. The public is can be fearful of this technology and angry ...something new and beautiful, to engage with the public in a meaningful way about science, and to have ...

...best way to share these improvements? You could publish a paper that happens to include your improvements...manuscript to get reviewed and edited prior to publication, and this will delay the dissemination of your...your improved protocol. Many scientists, publishers, and small start-ups are turning to software apps to... makes it easy to annotate protocols, make them public for others to use, and add your own comments. Currently...online protocol repository hosted by the Nature Publishing Group. Like the previously mentioned sites, NPE...charge to scientists by allowing companies to pay publish their catalogs on the site. Like Labguru, Quartzy...Freedom: The Expansion of Data Sharing in Research Publications by Jim Woodgett        ...

...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...doi.org/10.1038/nbt.3609 Find plasmids from this publication at Addgene. Kim E, Koo T, Park SW, Kim D, Kim...10.1038/ncomms14500 Find plasmids from this publication at Addgene. Kleinstiver BP, Prew MS, Tsai SQ...10.1038/nature14592 Find plasmids from this publication at Addgene. Kleinstiver BP, Tsai SQ, Prew MS...doi.org/10.1038/nbt.3620 Find plasmids from this publication at Addgene. Hu JH, Miller SM, Geurts MH, Tang...10.1038/nature14299 Find plasmids from this publication at Addgene. Walton RT, Christie KA, Whittaker.../j.cell.2015.09.038 Find plasmids from this publication at Addgene. Additional Resources on the Addgene...

...small selection of data found in full-length publications. As early career researchers, we wondered how...previously used reagent performs well, or you might publish a replication experiment that you conducted as ...papers as they are not included in traditional publications. By submitting your data to a repository (Figshare...good for you and good for science ReFigures are published instantly and are editable by the author at any...long before a study with multiple patients was published, Zika researcher Christa Osuna collated Zika case...-registration, preprints, data, software, micropublications) in grant proposals. ReFigures are one such...

...seems like another dragon to slay on the way to publish your work in a scientific journal. The peer-review... to help decide whether work is suitable for publication in their journal. The early peer-review attempts...touchstone of the scientific method, ensuring that published work meets the accepted standards of reporting.... The code and the data can be deposited in the public on-line repository (like GitHub, Zenodo, Figshare...comments. Receiving a peer-review is already a humbling experience, having your years of hard work being...Addgene.org Learn about depositing plasmids pre-publication Find molecular biology protocols Find eBooks ...

...towards a career in science communication, an established field of inquiry with which I was not yet familiar...are sincerely interested in connecting with the public in a meaningful way, and not solely to ensure a...doing so—can prove invaluable in the pursuit of public engagement. Studying science communication is ... a scientific background and bring with them established interests and areas of expertise. Perhaps the... research topic on concussion in sport and the public’s perception of head injury risk. In completing ...of unsettled science. Understanding just how the public utilizes insights gleaned from scientific research...

...item. Given a specific Addgene ID # listed in a publication, you can quickly pull up the associated plasmid...plasmid page also helps researchers find other publications that have used the same plasmid through the ...section. Having IDs linked to these metadata and publications help to enable reproducibility of experiments...Additionally, proper citation of these Addgene IDs in published work allows readers to quickly track down the ...Resource Identifiers (CURIEs) combined with well-established prefix resolution services such as Identifiers.org... in the material and methods section of your publication. You should carefully read and follow the journal...

...kinase. This hybrid construct senses light, and an obligate chromophore allows the system to respond to various...photosensor senses various light inputs, and an obligate chromophore allows the system to respond. In far-red...Oct 11. PubMed. Find the plasmids from this publication at Addgene. Synthetic biology: engineering Escherichia...:441-2. PubMed. Find the plasmids from this publication at Addgene. A synthetic genetic edge detection....2009.04.048. PubMed. Find the plasmids from this publication at Addgene. Multichromatic control of gene expression...Oct 28. PubMed. Find the plasmids from this publication at Addgene. More Resources at Addgene: Check...

...scientists”  These comments are well correlated with published data. For example, in 2010, less than 15% of postdocs...many notable contributions in this area include publishing the book ‘How Economics Shapes Science’ and contributing... Biology at Brandeis University, has recently published an article arguing against reducing the number...postdocs. Among other involvements, he helped to establish grants through the Sloan foundation for postdoc-related...employ them, the diversity of trainee backgrounds, publishing from a young scientist’s perspective, and the...science and in the intersection between science and public policy. Follow David on Twitter @driglar.     References...

...activities with the public, so I reached out to people who were educating the public and organizing events...wanted a career in science outreach: engaging the public to promote an awareness and understanding of science...for researchers interested in connecting with the public. My experiences in grad school, though often called...outreach, not only can I easily share science with the public, but I can watch and learn from the experts in ...also been a great place to reach out to local establishments such as museums, professional organizations...

...sexism in science, problems with peer-reviewed publications and funding, bioethics in the age of CRISPR,...examines the inherent brokenness of scientific publishing and research funding. I’ve been aware of these... place. Can we address problems in scientific publishing without acknowledging the systemic sexism in ...to the steadily increasing distrust the general public has in science and scientists (as discussed in ...Readers unfamiliar with academia’s challenges with publication and funding may be dismayed to see the dirty ...and the marginalization of science, particularly public health and environmental science, by the Trump ...

...Institute, Public Domain, National Institutes of Health, Wikipedia user Aimecie~commonswiki, Public Domain...let alone the human cost. Here are just a few publications which have since been retracted due to cell ...profiles are available for all the major cell lines on public databases. Image attributions (from top to bottom...cell line provenance and identity. The Nature Publishing Group recently reiterated its strict stance on...immortal life of Henrietta Lacks. New York: Crown Publishers, 2010. 2. Gold, Michael. "A conspiracy of cells...

...Addgene ID number, your publication will be listed on our website as a “Publication Citing this Plasmid” ...upon other researchers being able to find their publications in the first place. As a curator of the thousands...make it easier for you to find the plasmids and publications relevant to your research interests. We do this...number as well as the reference for its original publication. To help facilitate this process, we explain ...

...that there’s a new CRISPR advance or technique published every week! One of the newest applications is ...fluorescent distances correlating with the previously established physical map. In comparing pairs of targets ~...Feb 23. Pubmed. Find the plasmids from this publication at Addgene. Dynamic imaging of genomic loci....2013.12.001. Pubmed. Find the plasmids from this publication at Addgene. A protein-tagging system for signal...Oct 9. Pubmed. Find the plasmids from this publication at Addgene. ...

...wanted to be able to build assemblies through covalent bonds so that the assemblies wouldn’t fall apart, even...irreversible protein-peptide interactions. In a study published in Proceedings of the National Academy of Sciences...advantage of peptide tags for building larger protein assemblies? Howarth: Peptide tags can be very generic; you...Proceedings of the National Academy of Sciences paper [published online on March 17] should cover it. Addgene: ...