We narrowed to 63 results for: HDR;

...genome via the homology directed repair pathway (HDR), be it a single nucleotide change, insertion of ...mutations by NHEJ and generating small mutations with HDR, when it comes to larger scale genome editing, such...

...: Homology Directed Repair and 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells Day ...

...the basic science behind homology directed repair (HDR) and how it’s used in CRISPR genome editing.   2....

...diversification of hybridoma-produced antibodies by CRISPR/HDR genomic engineering. Sci Adv. 2019 Aug 28;5(8):eaaw1822...

...sequence integrated through homology-directed repair (HDR) (1), or indel mutations resulting from non-homologous...

...enhance DNA repair via homology directed repair (HDR). This characteristic makes AAV vectors especially...

...addition to a researcher’s locus of interest. NHEJ- or HDR-induced mutations in ATP1A1 can render cells resistant...editing rates as high as 83% with NHEJ and 40-50% with HDR. Coselection efficiency is so high that some applications...

...more than 10 bp away from the insertion site, so HDR will be difficult if there’s no PAM near your insertion...

... (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 kb. Find the plasmids at...

...editing doesn't require homology-directed repair (HDR) machinery, and could thus be used in non-dividing...

...edit a gene using homology directed repair, since HDR-mediated gene editing is most efficient when target...

...protein) upon CRISPR/Cas9 homology directed repair (HDR) (Glasser et al., 2016). For TREE they engineered...

...endogeneous DNA through homology-directed repair (HDR) of a CRISPR/Cas9 induced double-stranded break. ...

...editing methods (most notably CRISPR/Cas mediated HDR) enables the tagging of endogenous genes close to...

...than methods relying on homology-directed repair (HDR), and insertion is scarless, unlike previous integrase-based...

...These precise edits tend to be more efficient than HDR (reviewed in Zhu et al., 2019). Both cytosine base...

.... These tools assess repair events by type (NHEJ/HDR) and track indels at a single locus, usually the ...

...plus luciferase-2A-Cre recombinase, and Kras G12D HDR template 60225 : control for AAV-KPL; sgRNA targeted...)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL) This plasmid contains two expression...

...eukaryotes in that Cas9-mediated cleavage induces HDR to directly modify a given gene. CRISPR-tranposons...

...Elizabeth Hubbard: Neurons involved in opioid withdrawal Elizabeth Hubbard is a fourth year Ph.D. candidate...the development of behavioral signs of opioid withdrawal before they occur.  First, she used a genetic...genetic method to tag neurons activated by opioid withdrawal with tdTomato and assessed cFos expression. She...fluorescence during morphine administration and withdrawal to explore how these cells may encode the negative...negative affect associated with drug withdrawal.  “Together, this project will allow us to better understand... how neuron activity changes following opioid withdrawal, providing insight into new methods for treating...