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Showing: 41 - 60 of 71 results

  1. CRISPR Plasmids - Parasites

    Type
    Collection
    ...homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower ...

  3. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to tag ... PX458 . Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology...), an alternative DSB repair pathway, instead of HDR, which makes it easy to construct a donor vector,... PITCh system is different from the conventional HDR-mediated gene knock-in. The detailed procedure can...

  4. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower ...introduce specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication ...

  5. CRISPR Guide

    Type
    Guide
    ...Modifications Using Homology Directed Repair (HDR) Figure 4: Overview of HDR HDR generates specific genomic edits ...but high-fidelity homology directed repair (HDR) pathway. HDR relies on copying DNA from a matching template... Both NHEJ and HDR have their uses, but we’ll focus on NHEJ first and come back to HDR . NHEJ is a naturally...that modulate HDR or NHEJ components, like miCas9 , Cas9-DN1S , or others, can increase HDR efficiency. ...base. These small precision edits can be done via HDR, base editing, and prime editing, and are often used...mutation that does not change the amino acid sequence. HDR efficiency is generally low (< 10% of modified alleles...Synchronizing cells or modulating the cell cycle can help as HDR takes place during the S and G2 phases. Chemically...

  7. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower ...introduce specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker...

  8. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower ...

  9. CRISPR Plasmids - Plants

    Type
    Collection
    ...homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower ...introduce specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker...

  10. CRISPR Guide

    Type
    Collection
    ...Modifications Using Homology Directed Repair (HDR) Figure 4: Overview of HDR HDR generates specific genomic edits ...but high-fidelity homology directed repair (HDR) pathway. HDR relies on copying DNA from a matching template... Both NHEJ and HDR have their uses, but we’ll focus on NHEJ first and come back to HDR . NHEJ is a naturally...that modulate HDR or NHEJ components, like miCas9 , Cas9-DN1S , or others, can increase HDR efficiency. ...base. These small precision edits can be done via HDR, base editing, and prime editing, and are often used...mutation that does not change the amino acid sequence. HDR efficiency is generally low (< 10% of modified alleles...Synchronizing cells or modulating the cell cycle can help as HDR takes place during the S and G2 phases. Chemically...

  11. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...FRT sites, among others. Homology-directed repair (HDR) allows us to create a variety of modifications in...specificity. Figure 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA complex produces a DSB at the target...genomic locus. Repair will proceed through NHEJ or HDR depending on the experimental conditions. Genome ...editing technologies that do not rely on NHEJ or HDR: Base editors : Base editors directly convert bases...break. This method displays higher efficiency than HDR-mediated editing. The first base editors converted...

  14. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...addition to a researcher’s locus of interest. NHEJ- or HDR-induced mutations in ATP1A1 can render cells resistant...editing rates as high as 83% with NHEJ and 40-50% with HDR. Coselection efficiency is so high that some applications...

  17. Hot Plasmids - August 2020

    Type
    Blog Post
    ... (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 kb. Find the plasmids at...
Showing: 41 - 60 of 71 results