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We narrowed to 272 results for: HTT

Showing: 41 - 60 of 272 results

  1. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Using CRISPR/Cas Systems. Science 339:819–823 . https://doi.org/10.1126/science.1231143 Doench JG, Hartenian...gene inactivation. Nat Biotechnol 32:1262–1267 . https://doi.org/10.1038/nbt.3026 Gurumurthy CB, Takahashi...Embryos. Current Protocols in Human Genetics 88: . https://doi.org/10.1002/0471142905.hg1508s88 Leidy-Davis...CRISPR/Zygote Injection Approaches. Sci Rep 8: . https://doi.org/10.1038/s41598-018-33408-9 Mizuno N, Mizutani...Adeno-Associated Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci.2018.10.030 Qin W, Kutny...Editing. Current Protocols in Mouse Biology 6:39–66 . https://doi.org/10.1002/9780470942390.mo150178 Qin W, ...Electroporation of Nuclease. Genetics 200:423–430 . https://doi.org/10.1534/genetics.115.176594 Yang H, Wang...

  2. Viral Vectors 101: Producing Your rAAV

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    .... Human Gene Therapy Methods, 23(1), Article 1. https://doi.org/10.1089/hgtb.2011.034 Benyamini, B., Esbin...Adeno-Associated Viral Vectors [Preprint]. Molecular Biology. https://doi.org/10.1101/2023.03.20.533580 Goodwin, M. ... Molecular Neurodegeneration, 15(1), Article 1. https://doi.org/10.1186/s13024-020-00361-z Grimm, D., ...recombinant AAV-2. Gene Therapy, 6(7), Article 7. https://doi.org/10.1038/sj.gt.3300946 Howard, D. B., &.... Human Gene Therapy Methods, 28(1), Article 1. https://doi.org/10.1089/hgtb.2016.180 Korte, J., Mienert.... Human Gene Therapy, 32(13–14), Article 13–14. https://doi.org/10.1089/hum.2020.120 Lock, M., Alvira,.... Human Gene Therapy Methods, 25(2), Article 2. https://doi.org/10.1089/hgtb.2013.131 Lock, M., McGorray...

  3. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    ... Integrated in Parallel. Cell, 154(4), 914–927. https://doi.org/10.1016/J.CELL.2013.07.018 Anzalone, A...breaks or donor DNA. Nature, 576(7785), 149–157. https://doi.org/10.1038/s41586-019-1711-4 Chen, P. J.,...manipulation. Nature Reviews Genetics, 24(3), Article 3. https://doi.org/10.1038/s41576-022-00541-1 Kim, H. K.,...performance. Science Advances, 5(11), eaax9249. https://doi.org/10.1126/sciadv.aax9249 Koeppel, J., Weller... determinants. Nature Biotechnology 2023, 1–11. https://doi.org/10.1038/s41587-023-01678-y Li, X., Chen...epigenetic manipulation of prime editing. Cell. https://doi.org/10.1016/j.cell.2024.03.020 Mathis, N.,... learning. Nature Biotechnology, 41, 1151–1159. https://doi.org/10.1038/s41587-022-01613-7 Mathis, N.,...

  4. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
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    ...sponsored":false,"url":{"content_id":null,"href":"https://www.addgene.org/depositor-collections/allen-institute-aav-enhancers...allen-institute-aav-enhancers/","href_with_scheme":"https://www.addgene.org/depositor-collections/allen-institute-aav-enhancers...for genetic access to cortical cell types. Cell. https://doi.org/10.1016/j.cell.2025.05.002 Hunker, A. ...perturbing striatal cell types and circuits. Neuron https://doi.org/10.1016/j.neuron.2025.04.035 Graybuck,...cell-subclass-specific labeling. Neuron, 109(9), 1449-1464.e13. https://doi.org/10.1016/j.neuron.2021.03.011 Mich, J. ...primate neocortex. Cell Reports, 34(13), 108754. https://doi.org/10.1016/j.celrep.2021.108754 Panigrahi...the known and the unknown. Genome Biology, 22(1). https://doi.org/10.1186/s13059-021-02322-1  Pennacchio...

  5. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
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    .... Science (New York, N.Y.), 339(6121), 819–823. https://doi.org/10.1126/science.1231143  Gaudelli, N. ...without DNA cleavage. Nature, 551(7681), 464–471. https://doi.org/10.1038/nature24644  Gaudelli, N. M., ...application. Nature Biotechnology, 38(7), 892–900. https://doi.org/10.1038/s41587-020-0491-6  Komor, A. C...double-stranded DNA cleavage. Nature, 533(7603), 420–424. https://doi.org/10.1038/nature17946  Lander, E. S. (2016...2016). The Heroes of CRISPR. Cell, 164(1), 18–28. https://doi.org/10.1016/j.cell.2015.12.041  Musunuru, ...England Journal of Medicine, 392(22), 2235–2243. https://doi.org/10.1056/NEJMoa2504747  Rees, H. A., Wilson...base editors. Science Advances, 5(5), eaax5717. https://doi.org/10.1126/sciadv.aax5717  Richter, M. F....

  6. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...methylated somatic enhancers. Nat Genet 52, 819–827. https://doi.org/10.1038/s41588-020-0639-9.  Feinberg A...from their normal counterparts. Nature 301, 89–92. https://doi.org/10.1038/301089a0. Galonska C, Charlton...methyltransferase footprints. Nat Commun 9, 597. https://doi.org/10.1038/s41467-017-02708-5 Hrit J, Goodrich...activity and function in development. eLife 7:e34870. https://doi.org/10.7554/eLife.34870.  Kaas GA, Zhong C... and Memory Formation. Neuron 79, 6 P1086-1093. https://doi.org/10.1016/j.neuron.2013.08.032.  Morita ...catalytic domain fusions. Nat Biotechnol 34, 1060–1065. https://doi.org/10.1038/nbt.3658. Sapozhnikov DM, Szyf...methyltransferase with CRISPR/dCas9. Nat Commun 12, 5711. https://doi.org/10.1038/s41467-021-25991-9. Spruijt CG...

  7. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
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    ...enhanced multiplex immunolabeling of brain. eLife 8: . https://doi.org/10.7554/elife.43322 Baker M (2015) Reproducibility...Blame it on the antibodies. Nature 521:274–276 . https://doi.org/10.1038/521274a Begley CG, Ellis LM (2012...preclinical cancer research. Nature 483:531–533 . https://doi.org/10.1038/483531a Bradbury A, Plückthun ...antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a Bradbury AM, Plückthun...validate recombinants once. Nature 520:295–295 . https://doi.org/10.1038/520295b Bradbury ARM, Trinklein... functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080/19420862.2018.1445456 Crosnier...mouse monoclonal antibodies. BMC Biology 8:76 . https://doi.org/10.1186/1741-7007-8-76 Greenfield, E.A...

  8. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    ...CRISPR-Cas system. Nucleic Acids Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520 Chavez A, Scheiman ...transcriptional programming. Nat Methods 12:326–328 . https://doi.org/10.1038/nmeth.3312 Chavez A, Tuttle M,...activators in multiple species. Nat Methods 13:563–567 . https://doi.org/10.1038/nmeth.3871 Konermann S, Brigham...engineered CRISPR-Cas9 complex. Nature 517:583–588 . https://doi.org/10.1038/nature14136 Perez-Pinera P, Kocak...transcription factors. Nat Methods 10:973–976 . https://doi.org/10.1038/nmeth.2600 Qi LS, Larson MH, Gilbert...Control of Gene Expression. Cell 152:1173–1183 . https://doi.org/10.1016/j.cell.2013.02.022 Sajwan S, Mannervik...system differ in target preference. Sci Rep 9: . https://doi.org/10.1038/s41598-019-54179-x Tanenbaum ME...

  9. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
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    ...content_id":null,"href":"https://datahub.addgene.org/","href_with_scheme":"https://datahub.addgene.org/"...inflammation. Nature Reviews Immunology, 10(5), 301–316. https://doi.org/10.1038/nri2761 Chiu, M. L., Goulet, D...Engineering therapeutics. Antibodies, 8(4), 55. https://doi.org/10.3390/antib8040055 Holliger, P., & Hudson...domains. Nature Biotechnology, 23(9), 1126–1136. https://doi.org/10.1038/nbt1142 Kang, B. H., Lax, B. M...maturation. Methods in Molecular Biology, 29–62. https://doi.org/10.1007/978-1-0716-2285-8_2 McMahon, C...Structural & Molecular Biology, 25(3), 289–296. https://doi.org/10.1038/s41594-018-0028-6 Packer, M. S...proteins. Nature Reviews Genetics, 16(7), 379–394. https://doi.org/10.1038/nrg3927 Wang, Y., Xue, P., Cao...

  10. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...retroviruses. Microbiological Reviews, 56(3), 375–394. https://doi.org/10.1128/MR.56.3.375-394.1992. PubMed PMID...Current State and Perspectives. Viruses, 13(7), 1288. https://doi.org/10.3390/v13071288. PMID: 34372494. Prussin...Environmental Science & Technology Letters, 2(4), 84. https://doi.org/10.1021/ACS.ESTLETT.5B00050. PubMed PMID...of the United States of America, 54(1), 137–144. https://doi.org/10.1073/PNAS.54.1.137. PubMed PMID: 4285422...precursor protein. The EMBO Journal, 16(16), 5113–5122. https://doi.org/10.1093/EMBOJ/16.16.5113. PubMed PMID:...virus DNAs. Journal of Virology, 14(5), 1132–1141. https://doi.org/10.1128/JVI.14.5.1132-1141.1974. PubMed.... Journal of Molecular Biology, 79(1), 163–183. https://doi.org/10.1016/0022-2836(73)90277-5. PubMed PMID...

  11. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
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    ...D3 receptors. Scientific Reports, 10(1), 21842. https://doi.org/10.1038/s41598-020-78827-9  Breen, C. ...ligand-binding assay. Scientific Reports, 6, 25769. https://doi.org/10.1038/srep25769  Grimm, J. B., & Lavis...fluorescent labels. Nature Methods, 19(2), 149–158. https://doi.org/10.1038/s41592-021-01338-6  Grimm, J. ... vivo imaging. Nature Methods, 14(10), 987–994. https://doi.org/10.1038/nmeth.4403  Hayashi-Takanaka, ... partner for live cell imaging. PLoS ONE, 9(9). https://doi.org/10.1371/journal.pone.0106271  Heinrichs...fluorescent dyes. Nature Cell Biology, 11(S1), S7. https://doi.org/10.1038/ncb1939  Liu, X., Yang, L., Long...mammalian cells. Biophysics Reports, 3(4–6), 64–72. https://doi.org/10.1007/s41048-017-0037-8  Lucidi, M.,...

  12. Plasmids 101: Broad Host Range Plasmids

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    ...Journal of Biological Chemistry, 273(14), 8447–8453. https://doi.org/10.1074/jbc.273.14.8447  Jain, A., & Srivastava...plasmids. FEMS Microbiology Letters, 348(2), 87–96. https://doi.org/10.1111/1574-6968.12241  Keen, N. T., ...in Gram-negative bacteria. Gene, 70(1), 191–197. https://doi.org/10.1016/0378-1119(88)90117-5  Lale, R....Molecular Biology (Clifton, N.J.), 765, 327–343. https://doi.org/10.1007/978-1-61779-197-0_19  Meyer, R...host-range IncQ plasmids. Plasmid, 62(2), 57–70. https://doi.org/10.1016/j.plasmid.2009.05.001  Meyer, ...Molecular & General Genetics: MGG, 152(3), 129–135. https://doi.org/10.1007/BF00268809  Prior, J. E., Lynch...Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit.22695  Schmidhauser, T. J....

  13. Finding nucleic acids with SHERLOCK and DETECTR

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    ...RNA-targeting CRISPR effector. Science 353:aaf5573 . https://doi.org/10.1126/science.aaf5573 Broughton JP, ... detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Chen JS, Ma ...-stranded DNase activity. Science 360:436–439 . https://doi.org/10.1126/science.aar6245 Gootenberg JS,... with CRISPR-Cas13a/C2c2. Science 356:438–442 . https://doi.org/10.1126/science.aam9321 Gootenberg JS,... Cas13, Cas12a, and Csm6. Science 360:439–444 . https://doi.org/10.1126/science.aaq0179 Myhrvold C, Freije...diagnostics using CRISPR-Cas13. Science 360:444–448 . https://doi.org/10.1126/science.aas8836 Niewoehner O, ...CRISPR-associated protein Csm6. RNA 22:318–329 . https://doi.org/10.1261/rna.054098.115 Shmakov S, Abudayyeh...

  14. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
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    ...and applications. Gene Therapy, 17(2), 150–157. https://doi.org/10.1038/gt.2009.135. PubMed PMID: 19847206...Expression. Viruses: Essential Agents of Life, 147–175. https://doi.org/10.1007/978-94-007-4899-6_7. PubMed Central...human cells. Nature Biotechnology, 31(9), 822–826. https://doi.org/10.1038/nbt.2623. PubMed PMID: 23792628...Journal of Clinical Investigation, 118(9), 3143–3150. https://doi.org/10.1172/JCI35798. PubMed PMID: 18688286...infection. Journal of Virology, 70(2), 721–728. https://doi.org/10.1128/JVI.70.2.721-728.1996. PubMed ...genes and local hotspots. Cell, 110(4), 521–529. https://doi.org/10.1016/s0092-8674(02)00864-4. PubMed ...American Society of Gene Therapy, 17(8), 1316–1332. https://doi.org/10.1038/mt.2009.122. PubMed PMID: 19491821...

  15. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
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    ... editing. Nature Biotechnology, 40(5), 731–740. https://doi.org/10.1038/s41587-021-01133-w Anzalone, A...breaks or donor DNA. Nature, 576(7785), 149–157. https://doi.org/10.1038/s41586-019-1711-4 Chen, P. J.,...editing outcomes. Cell, 184(22), 5635-5652.e29. https://doi.org/10.1016/j.cell.2021.09.018 Choi, J., Chen... editing. Nature Biotechnology, 40(2), 218–226. https://doi.org/10.1038/s41587-021-01025-z Doman, J. L...efficient prime editors. Cell, 186(18), 3983-4002.e26. https://doi.org/10.1016/j.cell.2023.07.039 Lin, Q., Jin...in plants. Nature Biotechnology, 39(8), 923–927. https://doi.org/10.1038/s41587-021-00868-w Nelson, J. ...efficiency. Nature Biotechnology, 40(3), 402–410. https://doi.org/10.1038/s41587-021-01039-7 Pandey, S.,...

  16. Plasmids 101: Gram Negative and Positive Bacteria

    Type
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    ...(red/pink) staining. Image credit: Mark Perkins https://www.flickr.com/photos/occbio/7092385559 For...these resources.   Bruckner, M. Gram Staining. https://serc.carleton.edu/microbelife/research_methods...Protocols. American Society for Microbiology (2005). https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-...Positive vs Gram Negative. Technology Networks (2019) https://www.technologynetworks.com/immunology/articles...) a000414 (2010). Cleveland Clinic. Gram Stain. https://my.clevelandclinic.org/health/diagnostics/22612...gram-stain Hardy, J. Gram’s Serendipitous Stain. https://hardydiagnostics.com/wp-content/uploads/2016/05...

  17. Hot Plasmids February 2024

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    ...Methods, 1–9. https://doi.org/10.1038/s41592-023-02085-6. PMID: 38036853. Preprint: https://doi.org/10.21203...Biotechnology, 1–4. https://doi.org/10.1038/s41587-023-02018-w. PMID: 38081970. Preprint: https://doi.org/10.21203...Nature Biotechnology, 10.1038/s41587-023-01756-1. https://doi.org/10.1038/s41587-023-01756-1. PMID: 37095348...derived from StayGold. ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs-3188559/v1.  Neurogenetic...brain. Nature Nanotechnology, 18, 1241–1251 (2023). https://doi.org/10.1038/s41565-023-01419-x. PMID: 37430038...in primates. Nature Communications, 14(1), 3345. https://doi.org/10.1038/s41467-023-38582-7. PMID: 37291094...

  18. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
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    ...transposon-encoded CRISPR–Cas system. Nature 577:271–274 . https://doi.org/10.1038/s41586-019-1849-0 Klompe SE, Vo...RNA-guided DNA integration. Nature 571:219–225 . https://doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova...transposons. Proc Natl Acad Sci USA 114:E7358–E7366 . https://doi.org/10.1073/pnas.1709035114 Rubin BE, Diamond... Bacteria Within Microbial Communities. bioRxiv https://www.biorxiv.org/content/10.1101/2020.07.17.209189v2...CRISPR-associated transposases. Science 365:48–53 . https://doi.org/10.1126/science.aax9181 Strecker J, Ladha...CRISPR-associated transposases.” Science 368:eabb2920 . https://doi.org/10.1126/science.abb2920 Vo PLH, Ronda ...multiplexed bacterial genome engineering. Nat Biotechnol. https://doi.org/10.1038/s41587-020-00745-y Zhang Y, Sun...

  19. Negative Can Be Positive: Open AAV Data with Addgene

    Type
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    ...comparison to AAV PHP.B. Gene Therapy, 25(5), 392–400. https://doi.org/10.1038/s41434-018-0028-5 de Backer, M...Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089/hum.2009.169 Gruntman, A. M., ...trials. Human Gene Therapy Methods, 26(2), 71–76. https://doi.org/10.1089/hgtb.2015.040 Initiative, F. B...Preparations. Human Gene Therapy Methods, 30(6), 214–225. https://doi.org/10.1089/hgtb.2019.105 Rumachik, N. G.,...and Clinical Development, 18(September), 98–118. https://doi.org/10.1016/j.omtm.2020.05.018 Tedersoo, L...scientific disciplines. Scientific Data, 8(1), 1–11. https://doi.org/10.1038/s41597-021-00981-0 Wright, J. ...formulation. Molecular Therapy, 12(1), 171–178. https://doi.org/10.1016/j.ymthe.2005.02.021 Zuk, P., Sanchez...

  20. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...outcomes and assisting sgRNA selection is available at http://www.sunlab.fun:3838/BE_SMART/. Even more new DNA...repair proteins. Nature Communications, 12(1), 1384. https://doi.org/10.1038/s41467-021-21559-9 Huang, M. E...translocation. Nature Cell Biology, 26(2), 294–304. https://doi.org/10.1038/s41556-023-01342-2 Koblan, L. ...learning. Nature Biotechnology, 39(11), 1414–1425. https://doi.org/10.1038/s41587-021-00938-z Koblan, L. ...reconstruction. Nature Biotechnology, 36(9), 843–846. https://doi.org/10.1038/nbt.4172 Kurt, I. C., Zhou, R....human cells. Nature Biotechnology, 39(1), 41–46. https://doi.org/10.1038/s41587-020-0609-x Yang, C., Dong..., Baden-Wurttemberg, Germany), 9(26), e2202957. https://doi.org/10.1002/advs.202202957 Yuan, T., Yan, ...
Showing: 41 - 60 of 272 results