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We narrowed to 325 results for: INO

Showing: 41 - 60 of 325 results

  1. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...highly conserved glutamine-fructose-6-phosphate aminotransferase (glmS) gene in the recipient bacteria due ...plasmids, and lack the ability to synthesize diaminopimelic acid (DAP), a component of the cell wall. Once...disease (S. aureus, Klebsiella pneumoniae, and P. aeruginosa) in addition to Vicrio casei, a bacteria isolated...Enterobacter aerogenes, Klebsiella pneumoniae, and P. aeruginosa (all opportunistic pathogens) were grown in presence...

  2. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...range of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells (even pathogenic...aptamers hold a few important advantages over their amino acid-based counterparts, antibodies and enzymes,...structure stabilizes a 4-hydroxybenzylidene imidazolinone (HBI)-like compound allowing it to fluoresce...derivative 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) with relatively low fluorescence in ...

  3. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ... oligonucleotides called morpholinos. When injected into zygotes, morpholinos complementary to a target...activity and does not work easily in zebrafish.) Morpholinos are a convenient and fast way to deactivate genes...whether a gene is deactivated via mutation or morpholino. This observation unlocked novel research into...

  4. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...catalyzes the conversion of a target ‘A’ to ‘I’ (inosine), which is treated as ‘G’ by cellular polymerases...converted to a G-C pair (Figure 1). As the cellular inosine excision repair is not as active as uracil excision... for rice crop improvement in the laboratory of Yinong Yang at PSU.   References 1. Komor, Alexis C., ...PMC5726555. 4. Xie, Kabin, Bastian Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing capability...

  5. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...toxin genes isolated from E. coli or Psuedomonas aeruginosa. These toxins were a mix of Type VI secreted ...tested their expression under both rhamnose- and arabinose-inducible promoters. Tight control of toxin expression...though Khetrapal et al. also demonstrated that the arabinose promoter worked too. Rhamnose induction relies...et al. "Precision-engineering the Pseudomonas aeruginosa genome with two-step allelic exchange." Nature...

  6. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...identified the 13 amino acid sequence required for BirA recognition and biotinylation. This 13 amino acid sequence...processes such as gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction. Biotinylation...Biotin’s exposed side chain can be cross-linked to amino acid functional groups in vivo via specialized chemicals...

  7. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ... that alter the nucleotide sequence but not the amino acid sequence of the translated protein.  These ... include the IPTG-inducible lac promoter, the arabinose-inducible pBAD promoter and the endogenous phage...communication and in vitro diagnostics.     References 1. Costantino N, Court DL. Enhanced levels of λ Red-mediated... 5. Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N. and Court, D. L. 2014. Recombineering: Genetic...

  8. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...Epitopes are between 5-8 amino acids long, with a typical length of 5 or 6 amino acids (Cruse et al., 2004...Antibodies may recognize a linearized sequence of amino acids, or only in their 3D conformation. Types of...and sensitivity, for example by changing certain amino acids to improve its stability. Additionally, the...

  9. CRISPR Guide

    Type
    Guide
    ...base editor that converts adenosine to inosine in RNA. Since inosine is functionally equivalent to guanosine...to small indels in the target DNA that result in amino acid deletions, insertions, or frameshift mutations...enzymes. hfCas9s were developed by mutating specific amino acid residues to reduce off-target editing. Some... prime editing, and are often used to change an amino acid, turn a promoter on/off, or add small protein...with a silent mutation that does not change the amino acid sequence. HDR efficiency is generally low (...editors have been engineered to convert adenosine to inosine, which is treated like guanosine by the cell, creating...soon followed, using an enzyme evolved to excise inosine to access the same repair pathway and convert A...

  10. NETRF

    Type
    Collection
    ...discover cures and more effective treatments for carcinoid, pancreatic, and related neuroendocrine cancers... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University Epigenetic... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley Bernstein Broad Institute Identifying...Altered Epigenetic States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

  11. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ... from donors. Apart from fibroblasts, human keratinocytes from hair pluck, peripheral blood cells, and...Chondrocytes [27]         Adipocytes [28] Keratinocytes [29] Photoreceptors [30] Otic hair cells [...of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol, 2008. 26(11): p. 1276-84. PubMed... human induced pluripotent stem cells into a keratinocyte lineage. Methods Mol Biol, 2014. 1195: p. 1-...

  12. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...construct, you clone all but one of your gRNAs into spectinomycin-resistant plasmids pX330S-2 to pX330S-(last ...systems use the Csy4 RNA nuclease from Pseudomonas aeruginosa. When overexpressed, Csy4 efficiently cleaves...and Cas9, which are separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. ...Addgene. 8. Xie, Kabin, Minkenberg, Bastian, & Yinong Yang. “Boosting CRISPR/Cas9 multiplex editing capability...

  13. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ... is the nucleobase component of the nucleoside inosine. Created with BioRender.com. Although it was...To avoid confusion, we usually use different terminology when discussing base editors, like calling it...capable of chemically converting adenosine (A) to inosine (I, treated as G by the cell). Surprisingly, the...and Resources Glossary We introduced a lot of terminology in this post! Here’s a quick refresher if you...

  14. Zhang Lab CRISPR Page

    Type
    Collection
    ... Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F. Cell ....top three significantly mutated genes in lung adenocarcinoma. Delivery of a single AAV vector ( AAV-KPL ...mutations, leading to macroscopic tumors of adenocarcinoma pathology. These plasmids as well as a backbone...CRISPR-Cas9 complex. Konermann S*, Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

  15. Bacterial Expression Systems

    Type
    Collection
    ...Staphylococcus aureus Tim Foster 36267 pBAD33.1 pBAD Arabinose Escherichia coli Christian Raetz 26098 pCW-LIC...smegmatis Matthias Wilmanns 84689 pMyBADC-kan pBAD Arabinose Escherichia coli , Mycobacterium smegmatis Matthias...Escherichia coli Cynthia Collins 84689 pMyBADC-kan pBAD Arabinose Escherichia coli , Mycobacterium smegmatis Matthias... bacteria Attila Karsi 18855 FLIParaF.Ec-250n Arabinose FRET fluorescence (CFP and Venus) Escherichia ...Cyclic di-GMP Fluorescence (GFPmut2) Pseudomonas aeruginosa Tim Tolker-Nielsen 90217 pMV762-Peredox-mCherry...

  16. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...domain is interesting because by changing certain amino acids you can change the specificity,” explained...explained Zhanar Abil of the University of Illinois at Urbana-Champaign. The PUF proteins include eight modular...consecutive base in its RNA target. By changing amino acids, it’s possible to produce a protein that binds...

  17. Molecular Biology Reference

    Type
    Guide
    ...within an organism. Proteins are made up of amino acids. Each amino acid is encoded for by 3 nucleotides termed... there are only 20 natural amino acids and 64 codon combinations each amino acid is encoded for by multiple...encodes for an amino acid. The genetic code is degenerate thus multiple codons code for each amino acid. There...mg/mL 200 µg/mL Kanamycin 50 mg/mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg...There are 20 amino acids plus a start and stop codon. Below you will find helpful resource tables about...genetic code. This table includes the nucleotide and amino acid code in addition to ambiguous bases and common... A or G S C or G V A, C, or G W A or T Y C or T Amino Acids Name Three Letter Code Single Letter Code ...

  18. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...and Cas9, which are separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. ...enzyme cloning. Streptomyces bacteria are more recombinogenic than E. coli, so this system functions more...effectively able to introduce point mutations in P. aeruginosa, Pseudomonas putida, Pseudomonas fluorescens,.../10.1038/s41467-019-11479-0 Peters JM, Koo B-M, Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins...

  19. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata A. J Bacteriol. ...the Type I-F CRISPR/Cas system of Pseudomonas aeruginosa (6). However, these genes only deactivate the...

  20. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...at all levels.      Jargon is field-specific terminology or language. This can refer both to terms that...that are only used in the field and terminology that is widely used but has a very specific use-case (definition... your career as a scientist underrepresented minorities in your local high school who are also students...
Showing: 41 - 60 of 325 results