We narrowed to 108 results for: K-Ras protein

...with permission.   Mutations in the spike protein are one of the key drivers in determining whether...the current vaccines are effective or not. This protein both elicits an immune response as the target of... S, Wilkinson E, Naidoo Y, Karim F, Ganga Y, Khan K, Bernstein M, Balazs AB, Gosnell BI, Hanekom W, Moosa....2020.12.015 Garcia-Beltran WF, Lam EC, St. Denis K, Nitido AD, Garcia ZH, Hauser BM, Feldman J, Pavlovic.... In their assay, the pseudoviruses express a luciferase reporter which is used as a readout for infection...

... a complex of two proteins: the large (L) catalytic protein and its cofactor VP35. VP30 is a transcription...viral proteins (compared to viral infection). Therefore, the roles/functions of individual proteins can ... PubMed Central PMCID: PMC103829. 2. Conzelmann, K. K. "Reverse genetics of mononegavirales." Biology ...transcribed in the cell when the appropriate viral proteins are provided in trans (see Other Important Components... they do not encode all of the necessary viral proteins. Minigenome systems have been developed for many...genome and transcribes viral genes. Four viral proteins are required for viral genome replication and ...approach. First, this would prevent the study of the proteins independently from each other (see Uses of Minigenome...

...re-exposure (2, 3). More specifically, specialized Cas proteins snip the foreign DNA into small fragments approximately...paste them into the CRISPR sequence. Separate Cas proteins then express and process the CRISPR loci to generate...out these functions using slightly different Cas proteins and PAM sequences. A total of eight evolutionarily...us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata A. J Bacteriol. 1987 Dec; 169 (... (2007). CRISPRdb. Date: 2013-09-18. Makarova K, Haft DH, Barrangou R, Brouns SJJ, Charpentier E, ... bacterial genome. Cas genes, which code for polymerases, nucleases, and helicases, were also disrupted...

...the folded protein (green region). Methanol dehydrates the protein, which can disrupt protein structure...and leaves many membrane-associated proteins in place. In contrast, Triton X-100 and Tween-20 are examples... (as the name suggests) cross-linking cellular proteins. Cross-linking is a chemical reaction that covalently... polymerize and lose its ability to cross-link proteins. As such, you will get the best results if you...solvents can be quite harsh - lipids and soluble proteins can be lost during fixation with these chemicals...these chemicals can also alter the structure of proteins, which could disrupt your target epitope (Fig....antibody binding. Formaldehyde cross-links the protein (red lines), which maintains structural epitopes...

...detection because they produce very little or no viral protein. To solve this problem, two strategies have been... HIV so that infected cells will produce viral proteins and die, either through cytotoxicity or the immune...expression. In cell lines that can produce HIV-1 toxic proteins, CRISPR/Cas9 SAM caused apoptosis, indicating ...However, this approach could permit some viral protein production, so researchers are also interested ...antiviral therapies target HIV-1 at the level of protein structure, CRISPR gRNAs require DNA sequence-specific...Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K, Hu W. CRISPR/gRNA-directed synergistic activation...Tedaldi E, Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination of HIV-1 Genomes from Human T-lymphoid...

...specifically curated for protein expression in K. phaffii with special attention to protein modifications which...successfully for protein localization and transport studies in HEK cells, recombinant protein purification...used to optimize protein secretion and expression in the biotech workhorse yeast — K. phaffii (formerly...great option for those interested in recombinant protein purification and metabolic engineering. If instead...library of parts but don’t need to add tags to your proteins, then the CIDAR Kit is an excellent option (Iverson...compatible with E. coli and/or cell-free systems. Protein expression in yeast MoClo is also for eukaryotes...formerly known as Pichia pastoris). If your goal is protein isolation, then the MoClo Pichia Kit will get you...

...CRY2) and calcium and integrin-binding protein 1 (CIB1) proteins. The goal of both groups was to create...composed of two fusion proteins: 1) the genomic anchor - an inactive, dead Cas9 protein (dCas9) fused to CIB1...form a functional protein due to being fused with high-affinity interacting proteins Coh2 and DocS, respectively...chimeric protein consisting of Cas9 and a photo-dissociable dimeric green fluorescent protein (pdDronpa... saCas9 protein. Stop light! Photoactive anti-CRISPRs halt Cas9 activity Anti-CRISPR proteins are a highly...photoswitchable proteins derived from the fungal photoreceptor, Vivid (VVD, N. crassa) (Kawano et al.,... Nihongaki et al., 2015 paCas9 Magnet proteins from N. crassa Split Cas9, Split Cas9 nickase, Split dCas9...

...fluorescent proteins in the nervous system. Livet J, Weissman TA, Kang H, Draft RW, Bennis RA, Sanes JR,...Brainbow-1.0 construct contains three fluorescent proteins: RFP (red), YFP (yellow) and M-CFP (membrane tethered...Brainbow-1.1 scales up the system to four fluorescent proteins, with recombination events specified by three ...principles remain the same. The first fluorescent protein in the construct, OFP (orange), is expressed when...invertible segments, each with two fluorescent protein coding sequences in opposite orientations. Cre-...Cre is removed from the system, the fluorescent protein whose coding sequence is closest to the promoter...fluorophores used are fluorescently distinct, their protein sequences are highly homologous, preventing design...

...few years, scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a, to develop simple,...has adapted natural RNase activity of the Cas13 protein to develop and optimize the method termed Specific...endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. RNA 22:318–329 . https://doi.org/10.1261/...S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F (2016) C2c2...Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara ...Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (2015) Discovery and Functional...sample is first amplified using RPA (recombinase polymerase amplification) or reverse transcriptase (RT)-...

...interacting with proteins such as transcription factors and chromatin modifiers. These proteins recognize and... Way, S. W., Oster, A., Stafford, D. A., Mich, J. K., Taormina, M. J., Martinez, R. A., Opitz-Araya, X...Nguyen, T. N., Garren, E., Bendrick, J. L., Kim, T. K., Zhou, T., Mortrud, M., Yao, S., Siverts, L. A., ...https://doi.org/10.1016/j.neuron.2021.03.011 Mich, J. K., Graybuck, L. T., Hess, E. E., Mahoney, J. T., Kojima...It takes a village! While you usually hear this phrase when discussing raising children, it can also be... interact with mediator complexes and the RNA polymerase at the promoter region of a gene to initiate ...developed, funded, and shared has no doubt changed drastically over the past few decades. But no matter how ...

...lymphocyte receptor signaling. Zhao K, Wang W, Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree GR Cell. 1998 ...170(5):956-972.e23 Screen identifies bromodomain protein ZMYND8 in chromatin recognition of transcription-associated...Epigenome CUT&RUN: An Improved Method for Studying Protein-DNA Interactions CRISPRainbow and Genome Visualization... NEAT1 RNA is essential for the structure of paraspeckles. Clemson CM, Hutchinson JN, Sara SA, Ensminger...

...mini counterpart label nearby proteins with biotin. The biotinylated proteins can then be isolated and identified...lysosomal-associated membrane protein 5 (LAMP5) — as well as cholinergic (choline acetyltransferase, ChAT) neurons. ...for Glypicans DNA editing with STITCHR Dynamic protein labeling with TurboCas Enhancer-AAV viral preps...our bacterial plasmid set for expressing tagged proteins, and stay tuned for mammalian expression and AAV...therapeutic potential. Our partners at the Institute for Protein Innovation (IPI) recently released a set of highly-specific...antibodies against three of the six human glypican proteins (GPC1, GPC3, and GPC4). All IPI antibodies undergo...structure. Glypicans are proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG), and are (typically...

... AAVs “unlock” and infect specific cells. This protein shell packages the vector’s genetic material, and...doi.org/10.1038/s41598-025-05518-8 Bish, L. T., Morine, K., Sleeper, M. M., Sanmiguel, J., Wu, D., Gao, G., ... Xu, Z., Zhang, W., Qiu, Y., Wu, Y., Ye, C., Lin, K., & Xu, F. (2023). AAV11 enables efficient retrograde...doi.org/10.3390/cells12050785 Kaludov, N., Brown, K. E., Walters, R. W., Zabner, J., & Chiorini, J. A....Y., Viswanathan, S., Gaj, T., Lavzin, M., Ritola, K. D., Lindo, S., Michael, S., Kuleshova, E., Ojala,... Walters, R. W., Yi, S. M., Keshavjee, S., Brown, K. E., Welsh, M. J., Chiorini, J. A., & Zabner, J. (...Song, X., Wang, G., Su, X.-D., Bailey, C. G., & Rasko, J. E. J. (2025). An alternate receptor for adeno-associated...

...which “bait” proteins are tagged with Protein A-Renilla luciferase and the “prey” proteins are tagged with...2015. PubMed PMID: 26479688. RAS Pathway Clone Collection 2.0 Ras proteins are small GTPases involved in...central role Ras proteins play in cell signaling. Understanding how molecules in the Ras signaling pathway...method for identifying protein-protein associations. This method uses a bait protein fused to a biotin ligase...PrM N and C   Detecting protein-protein interactions with DULIP Proteins rarely act alone. Molecular...the NCI RAS Initiative, led by Dominic Esposito, has recently released a collection of 360 Ras pathway...ligase to biotinylate proximate proteins in the cell. These candidate proteins can be enriched via biotin ...

...many of the specifics - promoters used, precise protein sequences or domains, and methods of delivery -... at CRISPR. References Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V (2013) Plant genome editing ...10.1128/mmbr.67.1.16-37.2003 Khatodia S, Bhatotia K, Passricha N, Khurana SMP, Tuteja N (2016) The CRISPR...TTS, Sweeney CJ, Koman VB, Wong MH, Bohmert-Tatarev K, Snell KD, Seo JS, Chua N-H, Strano MS (2019) Chloroplast-selective...Wang C, Liu P, Lei C, Hao W, Gao Y, Liu Y-G, Zhao K (2016) Enhanced Rice Blast Resistance by CRISPR/Cas9...editing technologies especially in human cells. In contrast to homology directed repair, these methods do ...

...tissues, eliminating the need to deliver the Cas protein altogether. Platforming AAV Interest in interrogating...https://doi.org/10.1038/s41596-022-00700-y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski...Chamberlain, J. S., Corey, D. P., Aryee, M. J., Joung, J. K., Breakefield, X. O., Maguire, C. A., & György, B....Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J., & Kampmann, M. (2023). CRISPR screening...lentiviral vectors being packaged using VSV-G. In contrast, AAV can be packaged in a variety of capsids. ...

...covalent modifications to DNA or histones, the proteins that help package DNA, were associated with gene...With the help of guide RNAs, fusions between Cas proteins and the epigenetic modifier could be targeted ...cytosine demethylation of DNA. However, several proteins in the DNA oxidation and repair pathways work ...NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly large protein, and this fusion allows for packaging into viral...exhibits increased PAM flexibility, and this fusion protein allows for LSD1 targeting to a wider set of genomic...methylation in vivo using an engineered dCas9-MQ1 fusion protein. Nature Communications 8: . https://doi.org/10.1038...endogenous genes using programmable TALE-TET1 fusion proteins. Nature Biotechnology 31:1137–1142 . https://doi.org...

...modified reprogramming factor cocktail, and viral protein EBNA-1. Methods for reprogramming hiPSCs from ...EBNA-1 sequence that encodes for the DNA binding protein EBNA-1 [7]. These two sequences are necessary and... of the vectors in human cells [8]. The EBNA-1 protein recognizes OriP and induces amplification of the...transiently. The transiently expressed EBNA-1 increases protein expression from the other episomal plasmids. When...PubMed PMID: 14564000. 5. Seki, T., S. Yuasa, and K. Fukuda, Generation of induced pluripotent stem cells...29(5): p. 547-57. PubMed PMID: 25228828. 7. Okita, K., et al., An efficient nonviral method to generate...is easily and routinely drawn from patients in contrast to the process of collecting fibroblasts, which...

...very minimum requires an Argonaute protein. This Argonaute protein is what binds the small RNA and either...cleaves the target mRNA or recruits other silencing proteins. Figure 1: Comparison of shRNA and miRNA...10.1038/nbt.3947 Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998...10.1093/nar/gkab1256 Lee, Y., Kim, M., Han, J., Yeom, K., Lee, S., Baek, S. H., & Kim, V. N. (2004). MicroRNA...An oldie but a goodie — a phrase used to refer to something that has fallen out of fashion but is still...experiments. shRNA plasmids typically use RNA polymerase III promoters, which transcribe small RNAs. The..., shRNA plasmids are being designed using RNA polymerase II promoters. The design of these plasmids is...

...Visualizing Protein–Protein Interactions Easy Split Fluorescent Proteins for Studying Protein–Protein Interactions...Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to top Protein Visualization...that can be used for protein purification, protein visualization, study of protein interactions, controlled...Bacterial Expression Systems Protein Purification Protein Visualization Protein Interactions Controlled Expression... out more! Protein Purification To study the function, structure, and activity of a protein it is generally...biofactories to express plasmids and produce proteins in high yields. Proteins can then be extracted from the culture...process, proteins are often expressed alongside epitope tags, which can also later be used for protein detection...