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We narrowed to 206 results for: Kars;

Showing: 41 - 60 of 206 results

  1. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...their initial assumption. Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and.... Using computational observations, Koonin and Makarova found that Cas7-11 appeared to have derived from...Özcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS. Programmable...

  2. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...helps scientists achieve a high titer (reviewed in Joglekar and Sandoval, 2017). This high titer is one of...tropism, and advantages and disadvantages: Table 1 (Joglekar et al., 2017)  Table 1 (Gutierrez-Guerrero et ...Viruses 12:1016 . https://doi.org/10.3390/v12091016 Joglekar AV, Sandoval S (2017) Pseudotyped Lentiviral Vectors...

  3. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... 2843425.Takara Bio. Tet-On and Tet-Off systems: Second generation. https://www.takarabio.com/products... the system have been engineered and improved. Takara’s “2nd generation Tet-Off vectors (Tet-Advanced)...

  4. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Optogenetics Karl Deisseroth AV-1-26966P 26966-AAV1 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26968P 26968-AAV1 pAAV-Ef1a-DIO ChETA-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26971P 26971-AAV1 pAAV-CaMKIIa-eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26975 26975-AAV1 pAAV-CaMKIIa-hChR2(H134R)-mCherry Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26966P 26966-AAV5 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26969P 26969-AAV5 pAAV-CaMKIIa-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26973P 26973-AAV5 pAAV-hSyn-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth...

  5. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...found that La, which is ubiquitously expressed in eukaryotes, binds and stabilizes the 3′ tail of pegRNAs .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson, J. W., Newby...s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...

  6. Optogenetics AAV Preps

    Type
    Collection
    ...dependent 8 Karl Deisseroth 137144 pAAV-nEF-Coff/Fon-ChR2-mCherry nEF ChR2 mCherry Flp dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 26968 pAAV-Ef1a-DIO ChETA-EYFP EF1a ChETA EYFP Cre dependent 1, 5, 9 Karl Deisseroth...rg* Karl Deisseroth 26971 pAAV-CaMKIIa-eNpHR 3.0-EYFP CaMKII eNpHR 3.0 EYFP Constitutive 1, 9 Karl Deisseroth...Constitutive 2, 5 Karl Deisseroth 137151 pAAV-nEF-NpHR3.3-EYFP nEF NpHR 3.3 EYFP Constitutive 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137154 pAAV-nEF-Coff/Fon-NpHR3.3-EYFP nEF NpHR 3.3 EYFP Flp dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137156 pAAV-nEF-Con/Foff 2.0-iC++-EYFP nEF iC++ EYFP Cre dependent 8 Karl Deisseroth...pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Karel Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 Dlx...

  7. Control AAV Preps

    Type
    Collection
    ...PHP.eB Karl Deisseroth 114472 pAAV-hSyn-mCherry hSyn mCherry Constitutive 1, 2, 5, 8, 9, rg* Karl Deisseroth...Constitutive 8 Karl Deisseroth 131001 pAAV-hSyn-mScarlet Syn mScarlet Constitutive 1, 8 Karl Deisseroth 135630...dependent 9 Karl Deisseroth 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP Cre dependent 1, 2, 5, 9, rg* Karl Deisseroth...dependent 8 Karl Deisseroth 137133 pAAV-Ef1a-Con/Foff 2.0-mCherry EF1a mCherry Cre dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137162 pAAV-Ef1a-Con/Foff 2.0-EYFP EF1a EYFP Cre dependent 8 Karl Deisseroth..., rg* Karl Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP Flp dependent 1, 2, 5, 8, 9, rg* Karl Deisseroth...dependent 8 Karl Deisseroth 137134 pAAV-Ef1a-Coff/Fon-mCherry EF1a mCherry Flp dependent 8 Karl Deisseroth...

  8. Retrograde AAV viral preps

    Type
    Collection
    ...mCherry Control Karl Deisseroth 114469 pAAV-CaMKIIa-mCherry CamKII mCherry Control Karl Deisseroth 50459...Cre-dependent Control Karl Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP, Flp-dependent Control Karl Deisseroth ...Control Karl Deisseroth 114471 pAAV-Ef1a-fDIO mCherry EF1a mCherry, Flp-dependent Control Karl Deisseroth... Recombinases Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Karl Deisseroth 55637...Recombinases Karl Deisseroth 55638 pAAV-EF1a-vCre EF1a VCre expression Recombinases Karl Deisseroth 87306...Optogenetics Karl Deisseroth 26973 pAAV-hSyn-hChR2(H134R)-EYFP Syn Activator Optogenetics Karl Deisseroth...Optogenetics Karel Svoboda 26975 pAAV-CaMKIIa-hChR2(H134R)-mCherry CamKII Activator Optogenetics Karl Deisseroth...

  10. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...useful. But they were barely effective at editing eukaryotic cells — at least, until they met the directed...doi.org/10.1038/s41586-019-1323-z Peters, J. E., Makarova, K. S., Shmakov, S., & Koonin, E. V. (2017). Recruitment...

  11. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ... pAAV-Ef1a-fDIO EYFP AAV5. AAV8, AAVrg Control Karl Deisseroth New serotype (multiple) rAAV-CAG...pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE AAV8 Optogenetics Karl Deisseroth New viral prep pAAV-CaMKIIa(0.4)-eOPN3...

  12. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...An overview of CRISPR's development from a prokaryotic immune system to a valuable genome engineering...revolution shows no signs of slowing down. This prokaryotic immune system is especially amenable to genome...offering flexibility and easy multiplexing/scaling. Prokaryotes have long utilized CRISPR as a powerful defensive...elucidated until 2007. Scientists hypothesized that prokaryotes used CRISPR as part of an adaptive immune system...PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16...system seems like a winning strategy to ensure prokaryotic survival, but it is not invincible. Bondy-Denomy...provides acquired resistance against viruses in prokaryotes. Science . 315(5819):1709-12. PMID: 17379808 ...

  13. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...architecture to every diagram portraying some aspect of eukaryotic DNA would become busy and potentially detract...me at the Keystone Chromatin meeting, especially Karen Reddy (@theReddyLab), Thoru Pederson, Joanna Wysocka...

  15. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving database, and...Structural Biology. https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf, Y. I., Iranzo, J., et al. (2019)...

  16. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...Why do scientists use yeast vectors? Yeast are eukaryotes and thus contain complex internal cell structures...technical advantages that come with working with prokaryotes. This includes but is not limited to: rapid growth...

  19. Design Tips for Prime Editing

    Type
    Blog Post
    .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson, J. W., Newby...j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...

  20. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der...plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, ...
Showing: 41 - 60 of 206 results