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  1. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...helps scientists achieve a high titer (reviewed in Joglekar and Sandoval, 2017). This high titer is one of...tropism, and advantages and disadvantages: Table 1 (Joglekar et al., 2017)  Table 1 (Gutierrez-Guerrero et ...Viruses 12:1016 . https://doi.org/10.3390/v12091016 Joglekar AV, Sandoval S (2017) Pseudotyped Lentiviral Vectors...

  2. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...Pancreas AAV6, AAV8 Wang et al., 2006 Retina 7M8 Dalkara et al., 2013 Skeletal muscle MyoAAV Tabebordbar...1359–1368. https://doi.org/10.1089/hum.2008.123 Dalkara, D., Byrne, L. C., Klimczak, R. R., Visel, M., ...Hantman, A. W., Looger, L. L., Schaffer, D. V., & Karpova, A. Y. (2016). A Designer AAV Variant Permits Efficient...

  3. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... 2843425.Takara Bio. Tet-On and Tet-Off systems: second generation. https://www.takarabio.com/products... the system have been engineered and improved. Takara’s “2nd generation Tet-Off vectors (Tet-Advanced)...

  4. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...found that La, which is ubiquitously expressed in eukaryotes, binds and stabilizes the 3′ tail of pegRNAs .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson, J. W., Newby...s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...

  6. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...useful. But they were barely effective at editing eukaryotic cells — at least, until they met the directed...doi.org/10.1038/s41586-019-1323-z Peters, J. E., Makarova, K. S., Shmakov, S., & Koonin, E. V. (2017). Recruitment...

  7. New Viral Vectors - Winter 2025

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    Blog Post
    ... pAAV-Ef1a-fDIO EYFP AAV5. AAV8, AAVrg Control Karl Deisseroth New serotype (multiple) rAAV-CAG...pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE AAV8 Optogenetics Karl Deisseroth New viral prep pAAV-CaMKIIa(0.4)-eOPN3...

  8. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...architecture to every diagram portraying some aspect of eukaryotic DNA would become busy and potentially detract...me at the Keystone Chromatin meeting, especially Karen Reddy (@theReddyLab), Thoru Pederson, Joanna Wysocka...

  9. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving database, and...Structural Biology. https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf, Y. I., Iranzo, J., et al. (2019)...

  11. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...Why do scientists use yeast vectors? Yeast are eukaryotes and thus contain complex internal cell structures...technical advantages that come with working with prokaryotes. This includes but is not limited to: rapid growth...

  12. Design Tips for Prime Editing

    Type
    Blog Post
    .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson, J. W., Newby...j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...

  15. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der...plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, ...

  18. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...Insect cells can also be used to produce complex eukaryotic proteins with the correct post-translational ...system to generate large quantities of recombinant eukaryotic proteins. Although many species of yeast can ...
Showing: 41 - 60 of 207 results