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We narrowed to 1,039 results for: LAS

Showing: 41 - 60 of 1039 results

  1. Hot Plasmids - November 2023

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. ...reference atlas efforts, including a list of validated antibodies, on their webpage: mAb3D Atlas.  .... Today, it's time for another edition of Hot Plasmids!  Here's what you'll find in this post:  New...., 2023 under CC-BY 4.0 License. Find PE6 plasmids here!  Doman, J.L., Pandey, S., et al. (2023)....therapies and medical applications.  Find DIRECTED plasmids here! Strebinger, D., et al. (2023). Cell type-specific...pulse-chase experiments, and more. Find LOV-Turbo plasmids here! Next, Qin and Cheah et al. described TransitID...LOV-Turbo into TransitID experiments.  Find TransitID plasmids here! These two developments open up new possibilities...

  2. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles...future improvements. Find PinkyCaMP plasmids and FRCaMPi plasmids! Fink, R., Imai, S., et al. (2024).... fat cells fight cancer Frugal CRISPR kit for classrooms Cell-surface proteomics for neurons and astrocytes...isolated, identified by mass spectrometry, and classified by source cell based on single-cell RNA-seq data...cell types, and tissues. Find cell-surface HRP plasmids here! Wu, L., et al. (2025) The cell-surface ...systems, both in vitro and in vivo. Find MitoTRACER plasmids here! Borcherding, N., & Brestoff, J. R. (2023... al. (2025) under CC BY license. With 143 plasmids deposited from this paper, a large menu of tool...

  3. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...applications. Find ATLAS plasmids here! Rivera, J. F., Huang, H., et al. (2025). ATLAS: a rationally designed...the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles... and AAV11 packaging plasmids Mapping anterograde synaptic connections with ATLAS Bright and photostable...Find Addgene’s AAV6 and AAV11 packaging plasmids here!   An ATLAS for anterograde synaptic connections ...fluorescent protein mGold2 By Mike Lacy Get your sunglasses — these proteins are bright! Most current yellow...experiments and see the difference! Find mGold2 plasmids here! Lee, J., Lai, S., et al. (2025). Bright... to the broad host range of TRV. Find TRV-TnpB plasmids here! Weiss, T., et al. (2025). Viral delivery...

  4. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. ...Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set for expressing tagged proteins...and AAV plasmid sets launching later this year!  Find Addgene's own Lentiviral backbone plasmids here! ... synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A., Negrean, A., Chen, Y., Iyer...powerful tools in a scientist's toolkit is the empty plasmid backbone: the DNA vector that contains everything... swap sequences effortlessly.  We built these plasmids for flexibility and speed, so you can design, ...previous integrase-based approaches. Find STITCHR plasmids here! Fell, C. W., Villiger, L., Lim, J., Hiraizumi...

  5. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ... Resistance Gene Plasmids 101: Yeast Vectors Plasmids 101: Mammalian Vectors Plasmids 101: The Promoter...other plasmids? If we all use the same molecular code, can I transfect any old bacterial plasmid into ...blog Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection Plasmids 101:..., where DNA replication begins, so a bacterial plasmid needs a compatible ori that will be recognized ...prokaryotic growth. Since there are no natural mammalian plasmids, mammalian vectors are even less similar to bacterial...transfection. In stable transfection, DNA from the plasmid is incorporated into the host cell’s genome, so...duration of the experiment Selection Once a plasmid is delivered to your target cells, you still need...

  6. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...selectivity. With 10 plasmids available as a kit, plus 21 optional accessory plasmids, the collection targets...precise plant breeding. Find dicot prime editing plasmids here! Vu, T. V., Nguyen, N. T., Kim, J., Song... proteins for mammalian cell expression. These plasmids will be useful not only for future studies of ...proteins of interest too! Find CLIP, CLAP, and SNAP plasmids here! Smith, E.R., Turner, E.D., Abdelhamid, ...al., 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent versions that...potential, ASAP5 is a great new option. Find ASAP5 plasmids here! Hao, Y. A., Lee, S., Roth, R. H., Natale...

  7. Hot Plasmids - January 2023

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ... missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ... Cas13. CRISPR-Csm degrades both nuclear and cytoplasmic mRNA with 90-99% knockdown efficiency across ... genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from Figure 3 Colognori...Colognori et al 2022.   Find CRISPR Csm plasmids here! Colognori D, Trinidad M, Doudna JA. Precise transcript...inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the activity ...2: Addgene’s western blot for mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP...

  8. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...Addgene blog:  Plasmids 101: What is a plasmid? Plasmids 101: Origin of ReplicationPlasmids 101: PCR...process known as plasmid multimerization. Plasmid Multimerization Early studies on plasmid topology recorded...ligate the plasmid, and re-transform the ligated plasmid to recover the monomeric form of the plasmid. Have... monomer of Plasmid 12259: pMD2.G (depicted on the left) is ~5.8 kb in length. The plasmid can exist on... have been utilizing full plasmid sequencing services to verify the plasmids, including long-read sequencing... the sequencing results for their plasmids, they found the plasmids were much longer than expected, and...tandem. Such plasmids are known as multimers, and they are the result of multiple plasmid copies combining...

  9. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...Deletions in a Plasmids Learn How to Verify Your Plasmid Resources on Addgene.org Find More Plasmid Cloning...may want to include extra extension time after the last cycle of PCR to make sure that the "A" gets added...

  10. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...positive clones, the last step is to mini-prep these clones and submit the plasmids for Sanger sequencing...the correct orientation or even if it’s in your plasmid backbone. Backbone-specific primers: A second...orientation of your insert or if the insert is in your plasmid Have to make a new primer pair for each insert ...about the size of the insert and if it's in the plasmid Can be used to screen clones that were created ...created with the same backbone because primers are plasmid dependent Doesn't provide information about the...insert Tells you if the insert is present in the plasmid Great for blunt end cloning You have to make...thermocycling program. One key difference is the plasmid DNA must be released from the bacteria in order...

  11. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...In a previous post from our Plasmids 101 series, we learned how the Cre-loxP recombination system can... even in the presence of Cre recombinase. This plasmid now specifically drives expression of mCherry instead...2286602. PubMed Central PMCID: PMC3406316. Find FLEx plasmids from these Addgene depositors: Ed Boyden Bernardo... plasmids... plasmid... plasmids 101... flex plasmid...

  12. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily...restriction digests for your insert (or donor plasmid) and plasmid backbone. Because you lose some DNA during...insert and 1μg of plasmid backbone. It is also critical that as much of the backbone plasmid as possible be... of the backbone plasmid (see below). You should treat your digested backbone plasmid with a phosphatase...ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It...“recipient plasmid : insert ratio” in the ligation reaction. 5. Purify the finished plasmid   Once it...DNA; filter the remaining plasmid DNA through a column that binds the plasmid DNA and lets other materials...

  13. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ... best bet is the Plasmid Map: We auto-generate a plasmid map based off of the plasmid sequence and feature...colleagues. Top of the plasmid page The ‘breadcrumb’ trail at the top of any plasmid page gives the hierarchy... to the individual plasmid page. The depositing lab page contains all the plasmids from the same lab while...contains all the plasmids deposited from the same publication. Next up, we have the plasmid name: This is...other plasmids with similar names in the collection, but you can use this name to find this plasmid from...A quicker way to find this plasmid through search is... The Addgene plasmid number: This is the unique... given to every plasmid in our catalog - if you know the catalog number of the plasmid you are searching...

  14. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    ... here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids sent to happy scientists around... you all the plasmids you need. We're focused on our mission – documenting your plasmids, storing them...500,000 plasmids go? Far and wide – that's where! Check out the map below which depicts where plasmids have...Sidney Street. Then the plasmids travel to labs around the world. We've shipped plasmids to more than 90,000...providing quality control and customer support, making plasmids easy for you to find and request in our online...shipping them to you quickly.   So, what does plasmid shipping look like at Addgene? Here's an inside... workings in Addgene's lab. We ship individual plasmids as transformed bacteria in stab culture format...

  15. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...previous posts for our Plasmids 101 series, we examined a number of important plasmid elements – promoters...Resources on Addgene.org Find Cre plasmids Find loxP plasmids  ... expression proceeds. One popular lox-stop-lox plasmid is from Tyler Jack's lab: Lox-Stop-Lox TOPO.  Cre-dependent...  plasmids 101...

  16. Plasmids 101: Protein tags

    Type
    Blog Post
    ...localization, or protease protection. Thus far Plasmids 101 has covered GFP and its related fluorescent... however, those are just one (admittedly large) class of common fusion protein tags. Biochemists and molecular...stability activity, can be readily purified using plasmids found in this paper (available at Addgene).   ...interest: Which Fluorescent Protein Should I Use? Plasmids 101: Green Fluorescent Protein (GFP) Fluorescent...   plasmids 101...

  17. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...with a plasmid that already has the multicistronic element and reporter cloned in. In these plasmids you ...interest. Some of the plasmids listed in the table above (and their related plasmids) are designed to have... a one-stop reference guide for plasmids. We've combined our Plasmids 101 blog posts and additional resources...desktop. The Plasmids 101 eBook is designed to educate all levels of scientists and plasmid lovers and ... an introduction to plasmids, allowing you to spend less time researching plasmid basic features and spend...May 11.) PubMed.   Read more Plasmids 101 posts or browse Addgene's Plasmid Guide for more molecular biology...techniques including co-transfection of two or more plasmids, the use of multiple or bidirectional promoters...

  18. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...More: Plasmids 101: Antibiotic Resistance Genes Plasmids 101: Origin of Replication All Plasmids 101 Articles...Although plasmids do not naturally exist in mammals, scientists can still reap the benefits of plasmid-based...their genomes. Unlike plasmids used for expression in yeast and bacteria, plasmids used for stable transfections...Expression Plasmids? Check Out Addgene's Empty Backbone Resource: Choosing Your Perfect Plasmid Backbone...origin of replication (ORI) will not allow for plasmid replication in mammalian cells, for example, and...In this blog post we will discuss how mammalian plasmids differ from their bacterial counterparts, including...transfected cells. Before getting into the mammalian plasmid components, it may be useful to describe the means...

  19. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...types of yeast plasmids are defined below: Yeast Integrating plasmids (YIp): These plasmids lack an ORI...our first few Plasmids 101 posts, we focused mainly on the elements required for plasmid maintenance within...not only the number of plasmids found in each cell, but also whether the plasmid gets integrated into the...integration. Yeast Episomal plasmids (YEp): These are most similar to bacterial plasmids and are considered “...simple gene replacement/mutation. Yeast expression plasmids used in the lab typically contain all the necessary...how the regulation of bacterial ORIs determines plasmid copy number within the bacterial cell. Similarly...included within a yeast vector determine how the plasmid is replicated and maintained within the yeast cell...

  20. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...mutation” field on the plasmid page (see plasmid 87904 for example). As many plasmids available from Addgene...blog Plasmids 101: protein expression Plasmids 101: E. coli strains for protein expression Plasmids 101...codon optimized by ordering plasmids containing them from Addgene. If a plasmid at Addgene contains a gene...
Showing: 41 - 60 of 1039 results