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  1. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...Research and Therapeutic Purposes. Annu Rev Virol. 2014 Nov;1(1):427-51. DOI: 10.1146/annurev-virology-031413...prior with the death of Jesse Gelsinger in an adenovirus-based gene therapy clinical trial gone horribly...destructive inflammatory immune response. At the time, adenoviral vectors were the most commonly used viral vectors...Virus (AAV) While other viral vectors such as adenovirus (AdV) and lentivirus (LV) are known to transduce...

  2. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ... RJ, Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov;72(11):8463-71. PubMed PMID: 9765382. PubMed Central...dividing cells. Another type of vector, based on adenovirus, could infect non-dividing cells, but without...

  6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...ways to circumvent this limitation: 1) the use of novel S. pyogenes Cas9 variants with varying PAM sequences... from IGI!) Synthetic S. pyogenes Cas9s with novel PAM recognition In 2015, Keith Joung’s lab performed...2015). From these screens, they identified four novel SpCas9 variants with altered PAM binding specificity...remaining variants (VQR, EQR, and VRER) recognize novel PAM sequences (shown above). The VQR, EQR, and VRER... Expanding the CRISPR toolbox The isolation of novel CRISPR proteins has and will continue to dramatically...

  7. Zhang Lab CRISPR Page

    Type
    Collection
    ...Agarwala V, Scott DA, Zhang F. Nat Protoc . 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub...Kempton HR, Dahlman JE, Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, Heidenreich M, Xavier...

  11. Adenovirus Guide

    Type
    Guide
    ...on working with adenovirus. Overview of Adenoviral Plasmid System AdEasy™ for Adenoviral Vector Construction...line with adenovirus? No, adenoviral vectors can only be used transiently. Are adenoviruses replication... Science Guides Adenoviral Guide Adenoviral Guide ...Frequently Asked Questions (FAQ) Glossary Adenoviral Components The adenovirus genome is a linear, 36-Kb double-stranded...transcripts. There are 57 accepted human adenovirus types, and most adenoviral vectors are based on Ad5. Ad5-based... use the Coxsackie-Adenovirus Receptor (CAR) to enter cells. Recombinant adenovirus has two genes deleted...transfer) vectors and adenoviral vectors. Find vectors for this system on our adenovirus plasmid page. The...

  13. General Transfection

    Type
    Protocol
    ...limited effect on cell growth. Last reviewed on: November 7, 2023...

  14. Gibson Assembly Protocol

    Type
    Protocol
    ...colleagues at the J. Craig Venter Institute developed a novel method for the easy assembly of multiple linear ...

  16. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Determination of Lentiviral Infectious Titer by a Novel Droplet Digital PCR Method. Hum Gene Ther Methods...

  17. TALEN Guide

    Type
    Collection
    ...Martin-Luther-University Halle-Wittenberg and Adam Bogdanove at Iowa State University published the nucleotide...zinc finger community ( see interview with Adam Bogdanove ), progress on TALe research has been swift. The...Figure 2: Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit. In this example, the final...technically challenging. Thanks to efforts by the Bogdanove group and Daniel Voytas’s group from the University...backbones in just a few steps ( Figure 2 ). The Bogdanove group also hosts web-based software to help scientists...hundreds of unique visitors every month. The Voytas/Bogdanove TALEN kit came online in the summer of 2011 and...Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Jul;39(...

  18. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Core Lentiviral and Adenoviral Vectors You may also like... Lentiviral Guide Adenoviral Guide Viral Vector... and copGFP from the PGK promoter Adenoviral Vectors The adenoviral transfer vectors contained within ...Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral Vectors Kazuhiro Oka and the Baylor College of...have kindly deposited a series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer...pAdenoX from Clontech and can be used to generate adenovirus expressing a variety of reporters as well as ...transformants will instead form white colonies. Adenoviral Vector ID Purpose pAdx-CMV-LacZ 73345 Expresses...

  19. Viral Vectors

    Type
    Collection
    ...their small packaging capacity. Adenovirus Plasmids | Guide Adenoviruses are commonly used as vaccines ...immunogenic response they induce. Some (oncolytic) adenoviruses are utilized for cancer therapy because of their...preferentially infect and kill cancer cells. Adenoviruses have high transduction efficiency and direct...4.5 Kb 18-26 Dividing/Non-dividing No* Very Low Adenovirus Transient double stranded linear DNA >8 Kb 105...Lentivector Toolbox Guide to Generating Recombinant Adenoviruses Eric Campeau's Viral Vector Guide University....2010.12.009. PMID: 21167871 Wold WSM, Toth K. Adenovirus vectors for gene therapy, vaccination and cancer...

  20. COVID-19 Resources

    Type
    Collection
    ...Disease-2019 (COVID-19) pandemic is caused by a novel virus strain from the Coronaviridae family called...speed to elucidate as much as they can about this novel virus. It is now known that SARS-CoV-2 entry, like...protease TMPRSS2. Image credit: Maya Kostman for the Innovative Genomics Institute Addgene COVID-19 Resources...coronavirus - A protocol for rapid detection of the 2019 novel coronavirus SARS-CoV-2 using CRISPR diagnostics:...2 preprints from medRxiv and bioRxiv Elsevier's Novel Coronavirus Information Center The Lancet COVID-...COVID-19 Articles Wiley Online Library - Covid-19: Novel Coronavirus Content Free to Access Collections of...2020, BioRxiv SARS-CoV-2 invades host cells via a novel route: CD147-spike protein . Cathepsin L - Bosch...
Showing: 41 - 60 of 357 results