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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 588 results for: Ret;

Showing: 41 - 60 of 588 results

  1. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Anti-Calretinin [L122/68R] Calretinin Human Mouse IgG2a 177469 Anti-Calretinin [L122/6R] Calretinin Human...220384 Calretinin [L122/110R] Calretinin Human Mouse IgG2a 220385 Calretinin [L122/117R] Calretinin Human...220386 Calretinin [L122/132R] Calretinin Human Mouse IgG2a 220387 Calretinin [L122/133R] Calretinin Human...220388 Calretinin [L122/136R] Calretinin Human Mouse IgG2a 220389 Calretinin [L122/20R] Calretinin Human...-CALB2/calretinin [L122/68R-2b] CALB2/calretinin Human Mouse IgG2b 206642 Anti-CALB2/calretinin [L122/...-CALB2/calretinin [L122/68R-1] CALB2/calretinin Human Mouse IgG2a 206691 Anti-CALB2/calretinin [L122/6R...Theta Human Mouse IgG2a 188159 Anti-Calretinin [L122/144R] Calretinin Human Mouse IgG2a 188160 Anti-TASK1...

  2. Promega Plasmid Collection

    Type
    Collection
    ...interaction, association, and dissociation. NanoBRET Assays NanoBRET (Link opens in a new window) is an energy...proximity in living cells. NanoBRET PPI assays use NanoLuc Luciferase as a BRET energy donor and HaloTag...window) and NanoBRET assays (Link opens in a new window) NanoLuc®, HaloTag®, NanoBiT®, and NanoBRET® are Promega...Promega collection are available in two formats: pretransformed in bacteria as an agar stab 20 µg purified ...HaloTag protein, labeled with the NanoBRET 618 fluorophore, as the energy acceptor. Live-cell reagents allow... time using full-length proteins or fragments. NanoBRET target engagement (TE) assays (Link opens in a...compounds is measured by competitive displacement of a NanoBRET tracer, reversibly bound to a NanoLuc luciferase-fusion...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones...Zebrafish Plasmids and Resources collection page Return to top Epitope Tag or Fusion Protein Tags and fusion...bacterial expression (Gateway) pYD1 - Expression, secretion, and display of proteins on the extracellular ...as TEV cleavable N-terminal His and NusA fusions Return to top Function Besides functions such as protein...expression, see our dedicated Lentiviral Plasmids page Retroviral Easy and safe to use, integrate into host genome...MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian retroviral gene expression with GFP or mCherry selectable...mammalian cells with the Tet-On or Tet-Off system Return to top Selectable Markers Regardless of your delivery...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...nickase) and single guide RNA 48137 : PX165; SpCas9 Return to top GeCKO Screening Library GeCKO are pooled...for more information. Full references are below. Return to top Cas9 SAM transcriptional activation plasmids...sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing The Zhang Lab...fusion facilitates FACS sorting of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9 is a versatile...designed based on the target site sequence (20bp). Return to top References SpCas9 plasmids Multiplex Genome...CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini ...10.1038/nprot.2013.143. Epub 2013 Oct 24. PubMed . Return to SpCas9 plasmids GeCKO Library Genome-scale CRISPR-Cas9...

  5. Plasmids for Stem Cell Research

    Type
    Collection
    ...MMLV-derived Retrovirus Human Yamanaka factors for generating human iPS cells; retroviral expression of...MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS cells; retroviral expression..., fully-differentiated adult somatic cells are returned to a pluripotent state using a cocktail of factors...11(3):373-86. Jaenisch Fibroblasts Hepatocytes Retroviral Mouse Direct conversion of mouse fibroblasts ...nature10323. Crabtree Fibroblasts Motor Neurons Retroviral Mouse/Human Conversion of mouse and human fibroblasts...2527-32. Wernig Fibroblasts Neural Stem Cells Retroviral Mouse Direct reprogramming of fibroblasts into...Cell Stem Cell. 2019 Jun 6. Buganim Glial Cells Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron ...

  6. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...floxed-shRNA constructs, Cre can excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox...Flp-dependent expression systems and may affect the interpretation of your experimental results. Attenuated iCre...of Cre-lox and Flp-FRT — A Neuroscientist’s View Return to top Flp Recombinase Plasmids ID Plasmid Description...of Cre-lox and Flp-FRT — A Neuroscientist’s View Return to top Dre Recombinase Plasmids ID Plasmid Description...expressing Dre and other site-specific recombinases. Return to top Other Site-Specific Recombinase Plasmids...Description Gene/Insert Vector Type Promoter PI Return to top Recombinase-Dependent Empty Backbones Find...find other Cre-, Flp-, or Dre-dependent plasmids. Return to top Recombinase-Dependent Reporter Plasmids ...

  7. Viral Vector Guides and Plasmids

    Type
    Collection
    ... capacity. Gamma-retroviral Vector Guide | Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred... referred to as just retroviral vectors, have a relatively high transduction efficiency and only infect...genome, enabling long-term transgene expression. Retroviral vectors are popular for making stable cell lines...80–130 nm Dividing/Non-dividing Yes Low Gamma-retroviral Stable RNA < 8 kb 80–130 nm Dividing Yes Moderate...

  8. Open Enzyme Collection

    Type
    Collection
    ...Tth DNA Ligase 165574 pOpen-HSlig3 DNA Ligase III Return to top DNA Polymerases Addgene ID Plasmid Gene/...Polymerase 165573 pOpen-phi29pol phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid...165567 pOpen-XbaIR XbaI 165568 pOpen-NcoIR NcoI Return to top Reverse Transcriptases Addgene ID Plasmid...Thermus thermophilus (Tth) Reverse Transcriptase Return to top RNA Polymerases Addgene ID Plasmid Gene/...Polymerase 165576 pOpen-T3gene1 T3 RNA Polymerase Return to top Other Enzymes Addgene ID Plasmid Gene/insert...165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top...

  9. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...developing mouse retina. To aide in their studies of the regulatory networks involved in retinal development...plasmids to study the regulatory networks involved in retinal development. Depositor...Initiation of Otx2 expression in the developing mouse retina requires a unique enhancer and either Ascl1 or ...Simultaneous deletion of Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification...

  10. Recombinases AAV Preps

    Type
    Collection
    ...8, rg* Deisseroth *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...5, 8, 9, rg* Engel , Nectow 69570 pAAV-EF1a-N-CretrcintG EF1a none 1 Cepko 69571 pAAV-EF1a-C-CreintG EF1a...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...viruses would be helpful to the scientific community. Return to top...

  11. Chemogenetics AAV Preps

    Type
    Collection
    ...filtering options. *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova... dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t ...viruses would be helpful to the scientific community. Return to top...

  12. Antibody Plasmid Collection

    Type
    Collection
    ...mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected for...domains to obtain recombinant hybridomas, which secrete antibodies as Fab' fragments, isotype-switched ...antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining...libraries. Immunol Lett. 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for making synthetic ...-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions for other plasmids...

  13. Tetracycline Inducible Expression

    Type
    Collection
    ...Cre recombinase. Takuji Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression.... tTA-Advanced Tight TRE David Sabatini 19407 pTREtight2 Empty backbone for Tet-controlled expression ...promoter rtTA-Advanced Rudolf Jaenisch 25434 pMA2640 Retroviral Tet-On vector for CMV-driven rtTA with EGFP and...monosynaptic tracing. To be coinjected with pAAV-TREtight-mTagBFP2-B19G ( Plasmid #100799 ). Ian Wickersham...Baum, C., Loew, R., & Schiedlmeier, B. (2011). Retroviral and transposon-based tet-regulated all-in-one...

  14. Zebrafish Plasmid Collection

    Type
    Collection
    ... zebrafish cell surface receptors and secreted proteins. Return to top Other Resources Addgene Blog Posts...described below . ID Plasmid Description Gene/Insert PI Return to top Highlights of Zebrafish Tools in our Collection...-binary expression system for use in zebrafish - Bret Pearson Lab MAZe: a tool for mosaic analysis of ...for analyzing single cell genomics in zebrafish. Return to top Do you have suggestions for other plasmids...

  15. Control AAV Preps

    Type
    Collection
    ...Browse all Control AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...Serotype AAV1 AAV2 AAV5 AAV6 AAV8 AAV9 AAVrh10 AAV11 Retrograde AAV PHP.eB PHP.S PHP.V1 CAP-B10 CAP-B22 MaCPNS1...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...viruses would be helpful to the scientific community. Return to top...

  16. COVID-19 Resources

    Type
    Collection
    ...continually growing. ID Plasmid Description Industry PI Return to top Other Coronavirus Plasmids: SARS-CoV, MERS...materials are available only in the US at this time. Return to top Plasmids Encoding Mammalian Genes or Inserts...related plasmids see our Immunology Resource Guide . Return to top General Plasmid Tools SHERLOCK and DETECTR...Transcriptase plasmids from Andrew Ellington's lab. High-surety isothermal amplification and detection of SARS-...lab to ask them to deposit the plasmid with us. Return to top Pooled Libraries SARS-CoV-2 Spike (S) Ectodomain...

  17. Fluorescent Protein Guide

    Type
    Collection
    ...interest by fusing it to a fluorescent protein. FRET Find FRET pairs and standards to study protein-protein... empty backbones. Fluorescent Protein Kits cpFRET Kit: FRET based biosensors - Pertz Fluorescent Protein...

  18. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...HIV-based lentiviral particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles...Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus Envelope and Packaging Plasmids Reporter Plasmids...vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase...Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top Do you have suggestions for other plasmids...

  19. AAV for Neuronal Tracing

    Type
    Collection
    ...viral service encoding tools for monosynpatic retrograde neuronal tracing, to be used with modified rabies...be used with modified rabies virus to perform retrograde neuronal tracing. Note : Addgene does not distribute...deletion-mutant rabies virus AAV1 Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G AAV1 Wickersham Introduction to ..., Finke S, Conzelmann KK, Callaway EM. 2007a. Retrograde neuronal tracing with a deletion-mutant rabies...

  20. Microbiology Resources

    Type
    Collection
    ..., or by suggesting plasmids for the collection. Return to top Plasmid Kits for Microbiology Kits are sets...therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression - Pertz Lab...common S. cerevisiae lab strains - Ralser Lab Return to top Resources for Microbiology General CRISPR...Genome Database (SGD) (Link opens in a new window) Return to top Do you have suggestions for other plasmids...
Showing: 41 - 60 of 588 results