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We narrowed to 221 results for: Rras

Showing: 41 - 60 of 221 results

  1. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...produce functional R-mAbs. It was recognized that an aberrant IgG light chain transcript was expressed by some...the restriction enzyme BciVI was present in the aberrant light chain sequence, but was predicted to occur..., Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. A toolbox of IgG ...

  2. CRISPR References and Information

    Type
    Collection
    ...pAc-sgRNA-Cas9 PDF, 169 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF, 129 KB Marraffini Bacteria: pCRISPR...pSL1180polyUBdsRED PDF, 597 KB Zhang gRNA cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9...

  3. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...per each dCas9, SunTag uses a repeating peptide array to fused with multiple copies of VP64. By having... histone acetyltransferase domain capable of rearranging chromatin structure. This method has shown higher...Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable repression and activation...

  4. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ... test many variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered with... MoClo kit, is a prime choice for assembling an array of targeting constructs and delivering them to these...start from scratch! As you can imagine, there is an array of distinct goals within metabolic engineering, ...

  5. An Introduction to Adenovirus

    Type
    Blog Post
    ...transcription) is demarcated by viral genome replication (Harrach, 2014). Given that the early genes encode proteins...10.1080/22221751.2019.1690953. PMID: 31749409. Harrach, B. (2014). Adenoviruses: General Features. In ... Zhou, D., Lin, S. W., Bian, A., Zhi, Q. X., Iparraguirre, A., Lopez-Camacho, C., Wherry, E. J., & Ertl...

  6. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ... the past few years, scientists have created an array of base editing reporters that can help you do just...successfully used GO to induce the translation of an array of different reporters including mScarlet-I, luciferase...initiate protein expression, it can be used for an array of detectable proteins including fluorescent proteins...

  7. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...2009). Rodents have also been routinely used in an array of neuroscience research (Ellenbroek and Youn, 2016...biology tools to generate transgenic axolotls for an array of different studies. Elly Tanaka’s lab has constructed...in research (Krämer, 2015). As a result, a vast array of molecular tools, such as gene manipulation and...

  8. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...so that at least one might be useful in a broad array of pathogenic Proteobacteria. We placed the toxin..., Shigella flexneri, Enterobacter cloacae, and Serratia marcescens and have created dozens of deletions...development of allelic exchange vectors will produce an array of great options, and continue to improve the efficiency...

  9. Five Popular Model Organisms

    Type
    Blog Post
    ... has become an ideal model organism to study an array of topics including development, genetics, and the... A huge advantage of using the fruit fly is the array of genetic tools, such as the GAL4/UAS and LexA ...organisms. Worms have been used to investigate an array of biological systems and processes including the...

  10. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...209552 Knockout Human Doench 3rd 1, 2, or 4 49,766 arrays Broad GPP kinome Brunello 75314, 75315 (1 plasmid...Library 130630 Knockout Human Draetta 3rd 3-4 17,032 arrays Mouse Cardiac Transcriptional Regulators CRISPR...Library 123361 Knockout Mouse Chen 3rd 4 11,934 arrays Mouse SLC Knockout Library 141436 Knockout Mouse...

  11. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...pairs in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable of recording 11...reporter constructs or as the fully assembled pMemoryArray.   Yang et al., Nat Methods 2014 Dec;11(12...be used to visualize translocations, genome rearrangements and cancer-associated chromosome shattering...subpopulation of mitochondria is selectively labeled by irradiating the photoactivatable FP, then the overlap between... MYC, Flag), they can be deleterious and cause aberrant protein folding, expression, or even affect cell...

  12. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...and robust protocol for constructing TALE repeat arrays of any desired sequence, referred to as the REAL...restriction sites used for ligation into longer arrays. These 28 plasmids are available individually or...

  13. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ... nucleases in different cell types. Zinc finger arrays can be transferred in-frame to these vectors.... nucleases in different cell types. Zinc finger arrays created using ZFC reagents for either OPEN or modular...

  15. 8 Great Depositor Research Stories

    Type
    Blog Post
    ... depositors for sharing your science with us! Corralling and Cornering the Goldilocks Antibody: Guest ...drag-and-drop CRISPR editing tool for large inserts.  DAP arrays for multiplex gene editing: Guest blogger Qichen...

  16. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...plasmid Marraffini 42876 pCas9 BsaI E. coli, S. pneumoniae Chloramphenicol yes, cut Marraffini 44251 pgRNA-bacteria...

  18. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...pile of lab notebooks and binder on your desk. Arrange them in chronological order (or by project, if ...lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These programs can be...

  19. Cre-lox system

    Type
    Collection
    ...material will be rearranged. The schematic below shows the three types of rearrangements: inversion, deletion...Cre-Lox system and how it can be used for genetic rearrangements and recombination. Use Addgene's curated tables...

  20. The Strength of Story Telling

    Type
    Blog Post
    ...Humans are built to enjoy, remember, and listen to narratives — take advantage of that in your work The part...programmed to appreciate and understand. We build narratives around the self, too — when talking to friends...
Showing: 41 - 60 of 221 results