...to explore this role, how to seek out such an arrangement, and how to operate once in that position. What... plan as well. Making logistical and funding arrangements Visiting scientist positions take commitment...structured visiting program, you will have to make arrangements on your own. This will include, but is not limited...

...produce functional R-mAbs. It was recognized that an aberrant IgG light chain transcript was expressed by some...the restriction enzyme BciVI was present in the aberrant light chain sequence, but was predicted to occur..., Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. A toolbox of IgG ...

... test many variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered with... MoClo kit, is a prime choice for assembling an array of targeting constructs and delivering them to these...start from scratch! As you can imagine, there is an array of distinct goals within metabolic engineering, ...

...per each dCas9, SunTag uses a repeating peptide array to fused with multiple copies of VP64. By having... histone acetyltransferase domain capable of rearranging chromatin structure. This method has shown higher...Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable repression and activation...

...209552 Knockout Human Doench 3rd 1, 2, or 4 49,766 arrays Broad GPP kinome Brunello 75314, 75315 (1 plasmid...Library 130630 Knockout Human Draetta 3rd 3-4 17,032 arrays Mouse Cardiac Transcriptional Regulators CRISPR...Library 123361 Knockout Mouse Chen 3rd 4 11,934 arrays Mouse SLC Knockout Library 141436 Knockout Mouse...

...transcription) is demarcated by viral genome replication (Harrach, 2014). Given that the early genes encode proteins...10.1080/22221751.2019.1690953. PMID: 31749409. Harrach, B. (2014). Adenoviruses: General Features. In ... Zhou, D., Lin, S. W., Bian, A., Zhi, Q. X., Iparraguirre, A., Lopez-Camacho, C., Wherry, E. J., & Ertl...

... the past few years, scientists have created an array of base editing reporters that can help you do just...successfully used GO to induce the translation of an array of different reporters including mScarlet-I, luciferase...initiate protein expression, it can be used for an array of detectable proteins including fluorescent proteins...

... has become an ideal model organism to study an array of topics including development, genetics, and the... A huge advantage of using the fruit fly is the array of genetic tools, such as the GAL4/UAS and LexA ...organisms. Worms have been used to investigate an array of biological systems and processes including the...

...2009). Rodents have also been routinely used in an array of neuroscience research (Ellenbroek and Youn, 2016...biology tools to generate transgenic axolotls for an array of different studies. Elly Tanaka’s lab has constructed...in research (Krämer, 2015). As a result, a vast array of molecular tools, such as gene manipulation and...

...so that at least one might be useful in a broad array of pathogenic Proteobacteria. We placed the toxin..., Shigella flexneri, Enterobacter cloacae, and Serratia marcescens and have created dozens of deletions...development of allelic exchange vectors will produce an array of great options, and continue to improve the efficiency...

...pairs in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable of recording 11...reporter constructs or as the fully assembled pMemoryArray.   Yang et al., Nat Methods 2014 Dec;11(12...be used to visualize translocations, genome rearrangements and cancer-associated chromosome shattering...subpopulation of mitochondria is selectively labeled by irradiating the photoactivatable FP, then the overlap between... MYC, Flag), they can be deleterious and cause aberrant protein folding, expression, or even affect cell...

...and robust protocol for constructing TALE repeat arrays of any desired sequence, referred to as the REAL...restriction sites used for ligation into longer arrays. These 28 plasmids are available individually or...

... nucleases in different cell types. Zinc finger arrays can be transferred in-frame to these vectors.... nucleases in different cell types. Zinc finger arrays created using ZFC reagents for either OPEN or modular...

...open-source' protocol for making customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny S, Sander JD, Voytas...

...plasmid Marraffini 42876 pCas9 BsaI E. coli, S. pneumoniae Chloramphenicol yes, cut Marraffini 44251 pgRNA-bacteria...

... depositors for sharing your science with us! Corralling and Cornering the Goldilocks Antibody: Guest ...drag-and-drop CRISPR editing tool for large inserts.  DAP arrays for multiplex gene editing: Guest blogger Qichen...

...material will be rearranged. The schematic below shows the three types of rearrangements: inversion, deletion...Cre-Lox system and how it can be used for genetic rearrangements and recombination. Use Addgene's curated tables...

...uncharacterized genes that were proximal to a CRISPR array and the universal CRISPR integrase, cas1. They discovered...the cas14 gene together with the adjacent CRISPR array and intergenic regions containing the putative tracrRNA...

...pile of lab notebooks and binder on your desk. Arrange them in chronological order (or by project, if ...lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These programs can be...

...Humans are built to enjoy, remember, and listen to narratives — take advantage of that in your work The part...programmed to appreciate and understand. We build narratives around the self, too — when talking to friends...