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We narrowed to 456 results for: SUP

Showing: 41 - 60 of 456 results

  1. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...which we talk about below. Processing The viral supernatant is typically collected over the course of a couple...to 24 hours. Processing the virus-containing supernatant will depend on the virus type. AAV is typically...methods like ultracentrifugation, while lentivirus supernatants are centrifuged and filtered to eliminate any...than a P1 virus. Pro-tip! Centrifuge the viral supernatant in between passages to eliminate dead cells and...day or two, but unlike mammalian systems, the supernatant isn’t collected sequentially. This is because...majority of cells have died to collect your viral supernatant. Baculovirus replicates inside insect cells, ...ends in cell lysis, releasing the virus to the supernatant. The virus will reach peak production when roughly...

  2. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...t always indicate the presence of supportive data. To be supportive, the data must show that the antigen...This is the most usual level of evidence supplied to support the suitability of an antibody for its target... antibodies. It is also important to seek out supportive data on candidate antibodies from the manufacturer...a specific cytokine) known to either induce or suppress the expression of a target of interest, you can...However, the cell treatment will frequently induce/suppress the expression of many related proteins, not just...protein, show the same pattern of labeling, this is supportive of their specificity for the protein. A challenge...open science ecosystem. It has received in-kind support to produce this data from commercial antibody manufacturers...

  3. March for Science

    Type
    Blog Post
    ...hypothesis. The results of experiments can help support or reject a hypothesis. Looking at the data, scientists...trust in scientists if the march highlights data supporting unwanted results (14). Some loss of trust, I ...science) they come from many different places supporting many different positions. It would be detrimental...going change depending on the number of people supporting it.  3) Linking scientists to one particular ...world. There is no requirement that marchers or supporters be scientists – on the contrary, anyone and everyone...satellite marches. Anyone can join the marches or support the cause. For science to be integrated into politics...29). I tend to agree with him. Information on supporting scientists and science in government: 314 Action...

  4. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ....   PS: The superhero race is the perfect event for supporting AJ, who is a big superhero fan. This year...Cambridge Super Hero 5K on October 23rd. This is a great event at which people dress up like superheroes or ... their power from the emotion HOPE; a fitting superpower for those running on behalf of rare disease sufferers... superhero 5k...

  5. Addgene: The First Twenty Years

    Type
    Blog Post
    ...arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs and information across...AAV vectors, as well as developing and sharing supportive educational resources. By 2017, we had distributed... the COVID-19 pandemic, and Addgene pivoted to support researchers combatting the virus. Our COVID-19 ...Addgene began to think about how we could expand our support for the research community once more. Our ready-to-use...another plasmid-based product that, with Addgene’s support, could fill a community need for high-quality service...Neuroscience AntiBody Open Resource (NABOR) platform, supported by an NIH BRAIN Initiative. We also created a .... And we look forward to twenty more years of supporting you in your work, your research, and your discoveries...

  6. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...which the required adenoviral helper genes are supplied on two different packaging plasmids: the pHelper...your cells, wait 2–5 days and then collect the supernatant and/or producer cells for further processing....you can consider using unpurified AAV from the supernatant (Goodwin et al., 2020) or crude cell lysate (...  Purification To purify your rAAV from your supernatant, you can simply use polyethylene glycol (PEG)... 25 mL of 40% PEG solution to each 100 mL of supernatant (final concentration of 8%). If, however, you...sonication.  The obtained virions from cells and supernatant then need to be purified by density gradient ...particles containing a viral genome. While ddPCR is superior in precision and variability, qPCR is the more...

  8. AAV for Neuronal Tracing

    Type
    Collection
    ...the starter cell using the G-protein, which is supplied in trans . (3) Nascent G-protein coated RAVB is...

  9. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...benefit from some Addgene support? Please get in touch, as we aim to support a couple of events a year...undergraduates and PhD students so that they can help support each other and provide advice. The society organizes...involved with. The society provides talks and career support for a range of different scientific disciplines...alumni to mentor younger students in society and to support iGEM alumni-industry networking to promote the ... able to help with every request, we do aim to support events and scientific community activities throughout...

  10. Antibody Production

    Type
    Collection
    ...expressed for 7 days and then purified from the supernatant using Protein A or Protein G affinity columns...

  12. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...nanometers of the glass/water interface. Super resolution microscopy Super Resolution Microscopy allows for imaging...matter whether you are a sports photographer at the Super Bowl, a medical technologist taking an x-ray, or...spatial contrast (i.e. optical sectioning (2) or super-resolution (3). Fluorescence microscopy techniques...higher signal to noise ratio can be achieved (6). Super-resolution techniques such as Structured Illumination... these types of samples. Prior to the advent of super-resolution imaging, the light microscope was limited... appear as a fuzzy 200-300 nm spot when imaged. Super-resolution microscopy uses a number of optical and...

  13. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...10 that suppress APCs. T cells that would normally attack the cancer cells are also suppressed by chemical...immune cells and cells of epithelial origin. Suppressing function, creating tolerance, and evading recognition...by which tumors sidestep immune surveillance: Suppressing Function Antigen presenting cells (APCs) that...motif chemokine 22 (CCL22) and TGFβ. T-regs are suppressive immune cells that restrict the production and... change CD4+ Th cells into regulatory cells to suppress function. Creating Tolerance T-regs in the tumor...combination of tolerance marker blockade and suppression of tumor-aiding cytokines, for example, will ...

  14. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ... people who will support your professional development I had a wonderfully supportive mentor and graduate...they had my back. If your advisors are not as supportive of your career goals, find people who are. I ...mentioned, my graduate office staff was amazingly supportive. I talked to them about my interests and my ideas...professional organizations, and other nonprofits to gain support for CINSO. For instance, I tweeted to the Indiana...ever since, the State Museum has been the best supporter and most productive partnership CINSO has had....

  15. Antibody Plasmid Collection

    Type
    Collection
    ... in experiments such as immunoprecipitation and super-resolution microscopy. Two commonly used plasmid-based...

  16. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...the scientific support team at Synthego. Her work centers around educating and supporting novice to expert-level...was contributed by Jessica Roginsky, Scientific Support Lead at Synthego. Article source: Step-by-Step ...this problem. This tool was initially created to support the CRISPR analysis needs of Synthego’s scientists...other features and functionalities, please email support@synthego.com. Overview of ICE editing analysis ...CRISPR Gene Knockout Kit. Please contact us at support@synthego.com if you have any questions, and check...

  17. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...Singletons together would provide a structure and support network for people attending large meetings. A ...those who could not participate but provided moral support was invaluable.  Mixing metaphors and scientists...societies and conferences should strive to provide support to early career scientists, students, and those...ALL their members.  Shoutout to our mentors and supporters Thanks to Dr. Amanda L. Gunn  and Dr. Denise ...Singletons is especially grateful to its mentors and supporters during this first year. Mentors Amanda Gunn, ..., and Pragati Chengappa.  Financial and moral supporters Denise Akob, Sara Alexander,  Jill Bradley, Taylor...

  18. Microbiology Resources

    Type
    Collection
    ... cloning and expression systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors...

  19. Worm Expression Resources

    Type
    Collection
    ... cloning and expression systems) and therefore supplied as one item. Collections are plasmids grouped ...

  20. Plant Plasmids and Resources

    Type
    Collection
    ...promoter’ in the tables for species-specific tools). Support open science in plant biology - deposit plant plasmids...
Showing: 41 - 60 of 456 results