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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 451 results for: SUP

Showing: 41 - 60 of 451 results

  1. Microbiology Resources

    Type
    Collection
    ... cloning and expression systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors...

  2. Worm Expression Resources

    Type
    Collection
    ... cloning and expression systems) and therefore supplied as one item. Collections are plasmids grouped ...

  3. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...structure, separate chromosomes during mitosis, support transport of organelles and vesicles, and more....is not feasible or when the protein of interest supports a variety of processes in different cell types... (Aljiboury et al., 2023). Rab family proteins support vesicle trafficking and organelle dynamics, but...contexts are sure to reveal even more insights. Super-duper lipid editor And if recruiting proteins to...PLD). Now they present a set of variants called SuperPLDs with 10, 30, 50, and 100 times the activity of...other natural or synthetic head groups. The new SuperPLDs work with high efficiency in mammalian cells and...opening up a wide realm of possibilities. The CRY2-SuperPLD module is optogenetically recruited by different...

  4. Plant Plasmids and Resources

    Type
    Collection
    ...promoter’ in the tables for species-specific tools). Support open science in plant biology - deposit plant plasmids...

  5. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...the community as a whole could support Addgene as we have been supporting the community. While many were...Zuckerberg Initiative for supporting Addgene by providing a generous grant. This support will enable us to continue...We’re extremely proud of the work we’ve done to support COVID-19 research. Our collection of available ...operations for all ongoing processes are entirely self-supporting. We hire more staff as we need them to meet demand...approaching science funding organizations to request support for our operations until our normal work resumed...via Addgene. Perhaps if each of many grantors supporting research could provide Addgene with a small amount...Science, Cori Bargmann. "CZI is especially proud to support Addgene now, when their distribution of plasmids...

  6. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...linker mGFP-10-sREACh-N3 Monomeric EGFP attached to super-REACh via a 10 amino acid linker ACA Heterotrimeric...

  7. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...handy if a supervisor wants to see the full activity on a project. Can you meet the ELN support team? We... is likely to require on-going maintenance and support.” Besides that, academic institutions tend to think...study also showed that It is important that an ELN supports all types of data, which enables researchers to...property across various software providers used to supplement their paper notebooks. Instead, all of the important...use the ELN i.e. do you need a system that will support your lab’s growth in the near future? As most payable...years, it is also good to check how the ELN will support that. In addition, if you are working for a large...information regarding lab work with your colleagues, supervisors, clients or even students, your ELN should allow...

  9. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...that I was supremely proud of Nashville that morning. Despite the rain, about 4,000 supporters of science...seems to break everyday. Many researchers and supporters of science marched to advocate for scientist ...things work, test theories, and solve problems is supposed to be impartial. The best science happens when...°W – Vanessa Sung Hundreds of scientists and supporters in Montreal took to the streets on April 22 to...when I saw that there was going to be a march in support of science, I loved the idea and decided to go....Albany, Rep. Paul Tonko also came and spoke in support of science - he's one of the few members of Congress...science showed up to march. We had full support of local law enforcement, and, although there was at least...

  10. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...milestone. Viral service supporters We would like to thank the companies who supported this project with funding...seed money was used to build a completely self-supporting service so that Addgene is not dependent on grants... in accelerating the Viral Service. With their support we are able to provide faster access to a larger...number of viral preps. Visit our Sponsors and Supporters Page Thank you to all of our generous, sharing...Addgene repository. Without these scientists and the support of their technology transfer offices, Addgene would...Jonathan Ting’s lab from the Allen Institute have supported the AAV initiative from the beginning and have...Finally, we are blessed to have an incredibly supportive Board of Directors at Addgene. Our Board helps...

  11. Viral Production

    Type
    Collection
    ...Approximately 2 weeks post-thaw, cell culture supernatant is tested for mycoplasma contamination. To date...

  12. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ... The success of these projects allows us to support more new projects. Diversification is happening... quality and success. Customer and technical support Our customer and technical service brands continue...sharing, but we don’t have to worry about getting support from depositors. We regularly hear how much they...blessed with a large community of scientists who support us by depositing, requesting and spreading the ...Addgene Board of Directors is mature and poised to support our growth. Thanks to Joanna Brownstein (our outgoing...and Dave Root (incoming Chair) and our entire supportive Board, we have a robust committee process in ...thank them wholeheartedly for their service and support. The Addgene management team leads with excellence...

  13. Brain Armamentarium

    Type
    Collection
    ...function. Browse plasmids or viral preps created with support from the BRAIN Armamentarium Project below. Plasmids...

  14. CRISPR Guide

    Type
    Collection
    ...compatible with truncated gRNAs to increase specificity SuperFi-Cas9 - increases fidelity with reduced nuclease...consecutive binding and degradation of negatively supercoiled invader DNA by cascade and CAS3. Molecular Cell...

  15. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...which we talk about below. Processing The viral supernatant is typically collected over the course of a couple...to 24 hours. Processing the virus-containing supernatant will depend on the virus type. AAV is typically...methods like ultracentrifugation, while lentivirus supernatants are centrifuged and filtered to eliminate any...than a P1 virus. Pro-tip! Centrifuge the viral supernatant in between passages to eliminate dead cells and...day or two, but unlike mammalian systems, the supernatant isn’t collected sequentially. This is because...majority of cells have died to collect your viral supernatant. Baculovirus replicates inside insect cells, ...ends in cell lysis, releasing the virus to the supernatant. The virus will reach peak production when roughly...

  16. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...t always indicate the presence of supportive data. To be supportive, the data must show that the antigen...This is the most usual level of evidence supplied to support the suitability of an antibody for its target... antibodies. It is also important to seek out supportive data on candidate antibodies from the manufacturer...a specific cytokine) known to either induce or suppress the expression of a target of interest, you can...However, the cell treatment will frequently induce/suppress the expression of many related proteins, not just...protein, show the same pattern of labeling, this is supportive of their specificity for the protein. A challenge...open science ecosystem. It has received in-kind support to produce this data from commercial antibody manufacturers...

  17. March for Science

    Type
    Blog Post
    ...hypothesis. The results of experiments can help support or reject a hypothesis. Looking at the data, scientists...trust in scientists if the march highlights data supporting unwanted results (14). Some loss of trust, I ...science) they come from many different places supporting many different positions. It would be detrimental...going change depending on the number of people supporting it.  3) Linking scientists to one particular ...world. There is no requirement that marchers or supporters be scientists – on the contrary, anyone and everyone...satellite marches. Anyone can join the marches or support the cause. For science to be integrated into politics...29). I tend to agree with him. Information on supporting scientists and science in government: 314 Action...

  18. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ....   PS: The superhero race is the perfect event for supporting AJ, who is a big superhero fan. This year...Cambridge Super Hero 5K on October 23rd. This is a great event at which people dress up like superheroes or ... their power from the emotion HOPE; a fitting superpower for those running on behalf of rare disease sufferers... superhero 5k...

  19. CRISPR Plasmids - Tagging

    Type
    Collection
    ...your experiments can be found below and in the supplemental materials from the Doyon Lab’s publication: ...

  20. Addgene: The First Twenty Years

    Type
    Blog Post
    ...arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs and information across...AAV vectors, as well as developing and sharing supportive educational resources. By 2017, we had distributed... the COVID-19 pandemic, and Addgene pivoted to support researchers combatting the virus. Our COVID-19 ...Addgene began to think about how we could expand our support for the research community once more. Our ready-to-use...another plasmid-based product that, with Addgene’s support, could fill a community need for high-quality service...Neuroscience AntiBody Open Resource (NABOR) platform, supported by an NIH BRAIN Initiative. We also created a .... And we look forward to twenty more years of supporting you in your work, your research, and your discoveries...
Showing: 41 - 60 of 451 results