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We narrowed to 1,075 results for: TOR

Showing: 41 - 60 of 1075 results

  1. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...Viral Vectors 101: Viral Vector Elements Viral Vectors 101: An Introduction to AAV Viral Vectors 101:...heterologous envelope or a post-transcriptional regulatory element can be used for either type of retrovirus...clinical settings. AAV is also a cool tool in the laboratory! It is ideal for whole animal administration,...population. Some adenoviruses are known to cause respiratory disease across species, as well as cancer in ...strains have been engineered to induce a lower inflammatory response within hosts. Relative to the other... Galla M, Baum C, Schambach A. Gammaretroviral vectors: biology, technology and application. Viruses. ...

  2. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...(scAAV) vector. Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid...about Viral Vector Elements Resources on Addgene.org Read Our AAV Guide Find AAV Vectors Browse All ...make AAV ideal for human therapies and in vivo laboratory experiments ranging from transient gene expression...a type 2 ITR and a type 8 serotype. Almost all vectors contain type 2 ITRs, which comes from the AAV2 ...capsid (outer protein shell) used to package an rAAV vector; these capsids allow the virus to infect (or not...multiple small genes, you can design single rAAV vectors with multiple genes packaged in them and use elements...or second strand synthesis. In contrast, a scAAV vector with half the size of the ssAAV genome has a mutation...

  3. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ...We offer a variety of viral vectors, viral services and even a Viral Vectors eBook to get you started. ... terrains. They are, in fact, a choice delivery vector from basic science research all the way to clinical... long-term, and even inducibly depending on the vector and virus. Viral CRISPR-Cas9 tools have been...interest, turning expression off permanently. Viral vectors can also be used to express proteins. Viral expression...of interest, and often a selectable marker. This vector can express your construct in target cells transiently...mechanisms require a specific modification of the viral vector for your gene of interest – an shRNA sequence specific...

  4. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes pAAV-CAG-emiRFP670...retro) Looking for more viral vectors? Browse our in-stock viral vectors or consider our AAV Packaged ...Addgene’s viral vector services allow scientists to conduct experiments faster, skipping the preparation...production, purification, and titering. Our newest viral vector service, AAV Packaged on Request  has seen a fantastic...serotype, and a dTomato under the control of the E6 regulatory element, which targets it to VIP cortical interneurons...Hub can help, with over 100 user-submitted viral vector reports. And don't forget to use the link below...below to keep up with the latest in Addgene's viral vector services and resources!   This blog post was written...

  5. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...the first base transversion editor The story of base transversion editors starts with researchers in the...Cytosine Base Editor Converts C → T; the first type of base editor invented. ABE Adenine Base Editor Converts...a glycosylase base editor (GBE). GBE Glycosylase Base Editor A base editor that relies on DNA glycosylation...The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base...transversion editors. Red arrows indicate a conversion directly catalyzed by the base editor. Black arrows... depends on cellular factors. Multiple labs tried to identify beneficial factors to include in even better...DNA repair proteins to the base editor, they added the pioneer factor SOX2, which is capable of binding...

  6. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes pAAV.CAGFLEX... new preps available in the Addgene viral vector repository with a nice cup of hot chocolate is absolutely...retrograde Looking for more viral vectors? Browse our in-stock viral vectors or consider our AAV Packaged ...Hub can help, with over 100 user-submitted viral vector reports. And don't forget to use the link below...below to keep up with the latest in Addgene's viral vector services and resources!   This blog post was written...Addgene's Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  7. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    ... Lucky Thirteen: Guest Contributors of 2023 8 Great Depositor Research Stories Interested in contributing...Kits We are very grateful for each and every contributor to the blog. Thank you! More resources on the...

  8. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...researchers.  TALENs In 2010, transcription activator-like effector nucleases (TALENs) were engineered. TALENs... plague).  Base editors The major drawback of all the endonuclease-based gene editors (meganucleases, ...generate the prototypical base editor enzymes. The cytidine base editors (CBE) use a cytidine deaminase...the adenine base editors which can help accelerate the engineering of novel base editor proteins. She is... agricultural products. But it also made SciFi director Ridley Scott wonder what would happen if these...year after the discovery of meganucleases. The storyline involves the resurrection of dinosaurs by extracting...sequences is not ideal. For the early generation gene editors, new amino acid recognition sites had to be designed...

  9. New Viral Vectors - March 2024

    Type
    Blog Post
    ...made to our viral vectors repository this year (and it's only March!)  In our repository, you'll find the...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the...as seven vectors newly available in the AAV PHP.eB serotype. We've also added two viral vectors that can...these new vectors, and many more, in the table below.    Table 1: New ready-to-use viral vectors in the ...the Addgene repository  Name Serotype Category Depositor  Notes AAV-CAG-jGCaMP8s-WPRE AAV1 Biosensor...St-Pierre New viral vector pAAV-hSyn-JEDI-2P-WPRE AAV9 Biosensor St-Pierre New viral vector pAAV-CAG-RdLight1... Tian New viral vector pAAV-hSynapsin1-RdLight1 AAV9 Biosensor Tian New viral vector pAAV_hSynapsin_psychLight2...

  10. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...Patriarchi, T. (2023). Sensitive multicolor indicators for monitoring norepinephrine in vivo. Nature Methods...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been ...developing and depositing two new norepinephrine indicators: nLightG and nLightR (Kagiampaki et al., 2023...White insets indicate surface expression of the indicators over white dashed lines. Scale bars, 10 μm (HEK293T...nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains circularly permuted green fluorescent...trialed in two-photon in vivo experiments. nLight indicators are more sensitive to and selective for norepinephrine...This is important because alpha2-AR can provide inhibitory feedback in response to norepinephrine release...

  11. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    ... by sharing all the incredible guest posts and stories featured in our blog in 2023. Some of these were...Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic CapsidsResearchers: Cynthia Arokiaraj...

  12. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...recall, are the outer shell of an AAV vector, which determine the vector’s tropism, including its ability to...payloads. Since most systemic vectors are primarily derived from AAV9, the vectors have to be administered ...Hub and the CLOVER repository of published systemic AAVs to find commonly used vector cargo designs and... CNS (broad):  enhanced coverage for inhibitory versus excitatory neurons Mice, rats Yes (LY6A) ...AAV.CAP-B10 CNS: balanced coverage of inhibitory and excitatory neurons Mice, marmosets Unknown ...on the Addgene blog Viral Vectors 101: AAV Variables That Matter Viral Vectors 101: An Introduction to ...). Targeting AAV vectors to the central nervous system by engineering capsid–receptor interactions that...

  13. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...blog  Viral Vectors 101: Biosensors Viral Vectors 101: Viral Applications Viral Vectors 101: An Introduction... an enzyme co-factor, a crosslinking agent, a signaling ion, and an allosteric modulator. Because of its...jGCaMP7 and jGCaMP8.   Viral vectors So, what do viral vectors have to do with all of these cool ...also already packaged into viral vectors and ready to go. Pro tip: Vectors with ready-to-use AAV preparations... a calcium sensor Genetically encoded calcium indicators (GECIs) are fluorescent proteins linked to calcium...troponin C). Their fluorescent readouts are used to monitor calcium concentrations and transients in living...living cells and organisms. Unlike chemical indicators, these DNA constructs can be delivered to intact tissues...

  14. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...reversion of this mutation by an adenine base editor would restore antibiotic resistance. To find such a protein...not require any additional inhibitor protein like UGI in CBE. Base editors for the plant genome These ...2: Plant base editor platforms and their molecular components. Adenine base editors for plant We developed... developed plant adenine base editor (ABE) vectors to install A>G or T>C mutations in the plant genome...smaller vectors suitable for transient assays using protoplast transformation. Cytosine base editors for ...plant base editors. Plant CBE and ABE with expanded PAM compatibility All of the base editors mentioned...require other base editor platforms. Hua et al. constructed a number of plant base editors replacing the SpCas9...

  15. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ... Antibody Characterization Laboratory Cancer Any Data repository From the Frederick National Library...notes) Any Data repository Only accepts data for antibodies in Addgene’s repository  IBEX Knowledge-Base...Data repository See blog post mAb3D Atlas Mouse and human brain  IHC Data repository See...there are websites that can help: Antibody data repositories, which share validation data, and antibody search...using antibody search engines and antibody data repositories to find an antibody for your experiment. Search...available antibodies from many vendors, while data repositories share validation and experimental data to help...familiar with in Table 1.  Table 1: Antibody data repositories and search engines Website Targets Application...

  16. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes AAV-GfaABC1D-Cre... new preps available in the Addgene viral vector repository! This includes the first Addgene prep packaged... AAV9-X1.1!  We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab at the Allen Institute...carried out before. We have a bunch of other new AAV vectors as well — including next-generation biosensors ...multiple serotypes Looking for more viral vectors? Browse the full viral services collection or check... check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the...below to keep up with the latest in Addgene's viral vector services and resources!   This blog post was written...

  17. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the... the 20 additions we've made to our viral vectors repository since March.  Addgene is now offering an...This is an "optoGPCR" (coupled to Go, so it's an inhibitory opsin!) and due to its bistable nature, it enables... multiple serotypes Looking for more viral vectors? Browse the full viral services collection or check... check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the...below to keep up with the latest in Addgene's viral vector services and resources!   This blog post was written...

  18. Addgene Depositors Get More Citations

    Type
    Blog Post
    ... these plasmids. While clearly an outlier, this story had us wondering: is there a larger trend here? ...also get many more citations This isn’t the whole story, though. It would also be great to know how much... is just a first step. There are lots of other factors that could still be controlled for. As an example...

  19. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...plasmids use Rho-independent terminators, which include naturally occurring terminators, such as T7 and rrnB,...engineered terminators come close (>95%). For most purposes, however, any common terminator will suffice...suffice. Many commercial expression vectors use double terminators to reduce unwanted translation of downstream...require binding of termination factors. Although both are termination factor dependent, Polymerases I and...Posttranscriptional Regulatory Element Enhances Expression of Transgenes  Delivered by Retroviral Vectors. Journal...of yeast terminators of varying strength. A discussion of the effects of various terminators in the context...termination and polyadenylation? The role of the terminator, a sequence-based element, is to define the end...

  20. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...science stories. Here are some of our favorite spooky science stories. Six spooky science stories Zombie...Plasmid pumpkins, team costumes, and spooky science stories. Yes, please! At Addgene, we love finding ways ...Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the cell wall of green...forcing it out of hiding to become exposed to predators. Hungry birds spots the “caterpillar” and then... "Back from the dead; the curious tale of the predatory cyanobacterium Vampirovibrio chlorellavorus." ...
Showing: 41 - 60 of 1075 results