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We narrowed to 198 results for: aav vector plasmid

Showing: 41 - 60 of 198 results

  1. Viral Vectors 101: Biosensors

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    Blog Post
    ... Biosensor AAV Viral Vectors 101 eBook Resources on the Addgene blog An Overview of AAV Encoded Calcium...engineered into viral vectors, so if you want to use them, you’ll need to use the vectors (or redesign the ... to achieve in whole organisms, but AAVs make it possible. In AAV systems, different serotypes can be ...gamma retrovirus, or AAV as your vehicle, we’ve got you covered with packaging plasmids! References and ...genetically encoded, and luckily, we have pre-designed plasmids for these systems! Ion sensors One common biosensor...mechanisms. For one, many viral platforms, such as AAV or retrovirus, can either permanently integrate and...

  2. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post
    ... If there’s a plasmid or AAV tool that you can’t find, you can always suggest a plasmid to us, request... virus (AAV) and lentivirus are now available from Addgene. While super useful, these vectors require ...Addgene's Viral Service Find Retrograde AAV Browse Optogenetics Plasmids ...want to get into the field. Find Optogenetics Plasmids for Use in Neuroscience Research Neuroscience ...to generate new knockout mice, using adenoviral vectors to deliver IGF receptors to the brain, and probing...likely someway you can apply it in neuroscience. AAV tools from Addgene are already having an impact on...400 who signed up for our newsletter and a free plasmid) were very excited about our new viral services...

  3. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post
    ...largely precludes delivery by adeno-associated vectors (AAV). This issue was recently circumvented by the... construct for generating the SPH mouse. (B) AAV plasmids used to express CRE under neuron-specific promoters...individual neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific promoters...comprehensive CRISPR guide Find CRISPR plasmids and resources Find CRISPRa plasmids ...including transgenic mice, neuron-optimized viral vectors, and high-throughput screening approaches. Here...genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the protein ... inducible Cre recombinases can be introduced by AAV or by mating with Cre-expressing mice. Figure...

  4. Viral Vectors 101: Virus Safety

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    Blog Post
    ...transfer plasmid), the envelope (Env) gene on a separate plasmid (envelope plasmid), and the polymerase ...different plasmids. For a retroviral system this looks like the genetic cargo in one plasmid (the transfer...Gag structural protein on yet a third plasmid (packaging plasmid). Viral production then proceeds by transfection...and adeno-associated virus (AAV). The least risky of these four viruses is AAV – it is not known to cause...Viral Plasmids Which Type of Virus Should I Use? Resources on the Addgene blog Viral Vectors 101: ...integrate at chromosome 19q13.4 qtr. (AAVS1)). For these reasons, AAV is usually classified as a BSL-1 safety... transfection of all three plasmids into a producer cell line, such as HEK-293T, production of viral particles...

  5. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post
    ...Search for AAV vectors to use with your research. Visit Our AAV Guide Page for info on AAV components,...transfer to the rat CNS: AAV PHP. EB vector dose–response and comparison to AAV PHP. B." Gene therapy 25.5...nervous system (PNS) targeting AAV  Get 1st time AAV user tips from an AAV rookie  Learn about some considerations...stops viral vectors, such as adeno-associated virus (AAV), from reaching their targets. The AAV9 serotype...candidate capsids.  Are you interested in using AAV-PHP.B vectors? See Supplementary Table 3 in Huang et al....considerations when using AAVs Learn more about using AAV as a tool for gene expression in mammals. Resources...components, common uses of AAV, and much more! Find ready-to-use AAV from Addgene or check out protocols for packaging...

  6. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post
    ...Production of both AAV and lentivirus requires three plasmids — a transfer plasmid containing your gene...transfer plasmid. The transfer plasmids have different packaging capacities depending on the virus. AAV has...baculovirus plasmid. Production of baculovirus requires only two plasmids — your transfer plasmid and the ...Viruses AAV and lentivirus Baculovirus; can produce AAV and lentivirus if needed # of plasmids Three ...resources on the Addgene blog Viral Vectors 101: Producing Your rAAV AAV Titers: Where Do They Come From ...gene of interest plus two packaging plasmids (three packaging plasmids for 3rd generation lentivirus production...production. AAV and lentiviruses can be produced in insect cells, but they require cloning specific AAV or lentiviral...

  7. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    Blog Post
    ...our viral vector blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral preps...fluorescent GFP marker (CatCH-GFP). The lab deposited AAV plasmids containing the active promoters at Addgene. ...Performance of synthetic promoter AAVs in mice All of these synthetic promoter AAVs were first injected into the...outer part of the retina.  Find the AAV synthetic promoter plasmids! Comparing synthetic promoters performance...  Applications for synthetic promoter AAVs Besides restricting AAV transgene expression to particular cell... A toolkit of synthetic AAV promoters The Roska lab’s synthetic promoter AAVs allows for targeted gene...about using AAV for retinal gene therapy Find more about precise neuronal targeting using AAV technologies...

  8. Viral Vectors 101: Systemic Capsids

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    Blog Post
    ...blog Viral Vectors 101: AAV Variables That Matter Viral Vectors 101: An Introduction to AAV References...recall, are the outer shell of an AAV vector, which determine the vector’s tropism, including its ability...precious cargo space in AAV vectors. Dual expression systems, which use two or more AAVs to transduce the same...payloads. Since most systemic vectors are primarily derived from AAV9, the vectors have to be administered ... against AAV9-based vectors (and vice versa), enabling repeat dosing (Chen et al., 2023). AAV-BI30, developed...    Figure 1: AAV toolkit for targeted gene delivery to the brain. The AAV (a) capsid, (b) cargo...animal (i.e., 10 and 50 µL of vector at 1 x 1013 vg/mL titer), while AAV-PHP.S is typically used at a ...

  9. Viral Vectors 101: Viral Applications

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    Blog Post
    ...Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ...specifically AAV, are also being exploited as delivery mechanisms for gene therapy. The tractability of AAV, low...We offer a variety of viral vectors, viral services and even a Viral Vectors eBook to get you started. ... terrains. They are, in fact, a choice delivery vector from basic science research all the way to clinical...of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided on an application, our...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily... long-term, and even inducibly depending on the vector and virus. Viral CRISPR-Cas9 tools have been...

  10. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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    Blog Post
    ...principle of Tetbow In modern neuroscience, plasmid or viral vector-mediated gene delivery is more common than...four Tetbow plasmids (Addgene #104102 - #104105) using in utero electroporation or with AAVs (prepared ...troubleshooting your Tetbow experiment Plasmid concentrations and AAV titer - The XFP genes have to be introduced...highest color variations. Adjust the plasmid concentrations or AAV titer for the best results. The timing...Brainbow Learn about using AAVs for neuronal tracing Visit the viral vectors blog topic page Additional...than conventional transgenic strategies. In such vector-based gene delivery systems, the stochastic expression...sacrifice the animals and fix the samples. When you use AAVs, it typically takes a few weeks to achieve optimal...

  11. PEI Calculator for Planning AAV Packaging Transfections

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    Blog Post
    ...calculator for AAV packaging Select the plasmid molar ratio you would like to use for your AAV packaging ...names of the plasmids you plan to use Enter the base pair size of those plasmids Enter plasmid concentration...Using AAVs AAVs Are Great Tools for Gene Expression in Mammals Resources on Addgene.org Find AAV Packaging...PEI transfections for AAV packaging, considering using this PEI Calculator. AAV packaging typically requires... to making AAV packaging a little easier! Click here to download the PEI calculator for AAV packaging ...ratios below: 1 Transfer Plasmid : 1 pHelper: 1 RepCap OR 3 Transfer Plasmid : 5 pHelper: 2 RepCap Select...of each plasmid needed per dish or stack. Calculations are based on using 28 μg of total plasmid (Transfer...

  12. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post
    ...relevant plasmid depositors and their institutions to distribute viral preps derived from plasmids in the...distributing a small but growing catalog of ready-made AAV and Lentiviral preps. This new Viral Service represents...largest new initiative since we started distributing plasmids in 2004. We’ve already distributed over 500 viral... virus. Addgene is a 501(c)3 nonprofit and our plasmid service was started based on donations. That seed...depositors. Over 3,000 laboratories are sharing plasmids (with 16 labs currently sharing viral preps) through... lab from the Allen Institute have supported the AAV initiative from the beginning and have worked closely...Research Campus have provided valuable insight into AAV production. Finally, we are blessed to have an incredibly...

  13. Droplet Digital PCR for AAV Quantitation

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    Blog Post
    ...Since Addgene began producing AAV vector preparations, we have used quantitative PCR to evaluate the ...powerful tool for quantifying AAV for some time now. When titrating AAV by qPCR, viral DNA is amplified...posts related to viral vector protocols and tips Read all of our viral vectors blog posts Resources on...Learn our protocol for AAV titration by qPCR Browse our collection of viral vector related protocols Learn... is where droplet digital PCR (ddPCR) comes in. AAV titering using quantitative PCR Before we dive into...reactions with known quantities of DNA. Additionally, an AAV reference, a virus with a known titer, can be used...and both results would be valid. This is why the AAV reference is critical. It allows you to determine...

  14. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post
    ...deliver in low-capacity vectors like AAV. AAV (adeno-associated viral vectors) are commonly used for in..., these vectors have a low genetic capacity of ~4.5 kb. At 4.1 kb, SpCas9 barely fits into AAV when you...previously used SaCas9 (1053 aa/3.2 kb) with AAV vectors for efficient postnatal editing. LbCpf1 and AsCpf1...SpCas9. These nucleases should fit well into AAV vectors, especially given that Cpf1 gRNAs are also shorter... is well suited for genome editing.  Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche...potential to eclipse any of the CRISPR nucleases in AAV-based applications. Get More Cpf1 Background Here...Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver...

  15. Hot Plasmids February 2024

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    Blog Post
    ...an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...all applications. Addgene’s plasmid collection includes over 10,000 plasmids with a FLAG tag (!), but did...from variable region VIII of AAV9-X1.1 to that of AAV1. This viral vector has brain endothelial cell-specific...Importantly, AAV1-X1 is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice ... enhanced brain potency of AAV-PHP.eB.    Figure 4: AAV9-X1 and AAV9-X1.1 efficiently transduce... variants AAV.CAP-Mac for systemic gene transfer in primate brains Endothelial-specific AAV delivery Recombinant...

  16. Plasmids 101: FLEx Vectors

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    Blog Post
    ...of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector Elements Read Our Primer on Optogenetics...improvement of optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional uses for ...In a previous post from our Plasmids 101 series, we learned how the Cre-loxP recombination system can... even in the presence of Cre recombinase. This plasmid now specifically drives expression of mCherry instead...Since most optogenetic reporters are delivered using AAV injection, the majority of the cells surrounding ...2286602. PubMed Central PMCID: PMC3406316. Find FLEx plasmids from these Addgene depositors: Ed Boyden Bernardo... plasmids...

  17. What's New in CRISPR - Spring 2019

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    Blog Post
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...Biology Find plasmids containing these base editors Spatially and temporally regulated AAV-based genome...developed another AAV toolkit - this time using the Staphylococcus aureus Cas9 (SaCas9). This AAV-based genome...Molecular Neuroscience Find the AAV-based SaCas9 genome editing plasmids String assembly gRNA cloning...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...paper in Nature Communications Find the Cas12b plasmids  Plant base editing with a Cas9-adenosine deaminase...single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one overnight...

  18. Hot Plasmids: Spring 2025

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    Blog Post
    ... and AAV plasmid sets launching later this year!  Find Addgene's own Lentiviral backbone plasmids here...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. ...a scientist's toolkit is the empty plasmid backbone: the DNA vector that contains everything needed to...Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set for expressing tagged proteins...for successful experiments or therapies. Using AAV vectors with enhancers that drive cell-type-specific ...colleagues characterized a series of new enhancer-AAV vectors targeting specific classes of cortical and striatal... Dynamic protein labeling with TurboCas Enhancer-AAV viral preps targeting interneurons Glutamate indicators...

  19. Hot Plasmids - August 2020

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    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...They generated plasmids tagged with EB3 to mark the growing tips of microtubuli and plasmids tagged with ...and dCas9-VPR targeting plasmids can be found at Addgene. Check out these plasmids for piggyBac gRNA delivery...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head... Find the plasmids at Addgene The MoClo CRISPR/Cas Toolkit for plants includes 95 plasmids consisting...and dicots. Find the plasmids at Addgene! CRISPR RNP electroporation and AAV donor infection (CRISPR-READI...

  20. Viral Vectors 101: Transductions

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    Blog Post
    ...Production at Addgene Viral Vectors 101: What is a Virus? Viral Vectors 101: Viral Vector Elements ...adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned into the vector that you have...non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform a lipid-based...nucleic acid cargo of your choice. First, a viral vector is selected to perform the transduction with: either...produced, and viral particles are packaged, when the vector is expressed. Once the design and construction ... it’s time to make virus! The constructed viral vector containing the cargo of interest is then typically...typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK-293...
Showing: 41 - 60 of 198 results