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We narrowed to 51 results for: cdna expression plasmid

Showing: 41 - 51 of 51 results
  1. Illuminating Epigenetics with A FRET Based Biosensor

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    Blog Post
    ...gene is expressed in normal individuals (in a parent-of-origin-specific manner), but the expression or silencing...Epigenetics is the study of heritable changes in gene expression that do not involve any changes to DNA sequence...is wrapped around histones changes, then gene expression can change as well. The more frequently and tightly...compacted region and results in a loss of gene expression. In this way, genes in compacted regions are ...until now. Alice Ting’s lab has developed a new, plasmid-based biosensor to visualise changes in histone...opens up a new way to visualize changes in gene expression and may lead to the development of a whole new...Additional Resources at Addgene Find the Ting Lab plasmids at Addgene Browse More Fluorescent Protein Biosensors...
  2. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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    Blog Post
    ...available in a mammalian expression vector (pcDNA3) and a bacterial expression vector (pRSETB). Fabritius...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...about a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids! Light controls...Hahn's lab at the University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 ... the same number of fluorophores. You can find plasmids containing the cobalamin binding riboswitches ...the Reuben Harris lab has deposited a reporter plasmid that allows for rapid fluorescence-based quantification...
  3. Tetracycline Inducible Expression

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    Collection
    ...Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection. See Plasmid #21916 for ...dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9...Resources Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research Plasmids Blog: Inducible Promoters...gene expression and find plasmids for your experiments using tet- or dox-inducible systems. Plasmid...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Tetracycline Off Tetracycline...ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression systems. Left: natural TetR ...factors to further minimize background expression. Improved expression of the transactivator proteins in mammalian...
  4. Fluorescent Protein Guide: Empty Backbones

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    ...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...MiCy-N1 - Mammalian Expression MiCy-C1 - Mammalian Expression MiCy-pBAD - Bacterial Expression Return to top...Mammalian Expression sfGFP-C1 - Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP 486 510 ...1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - ... - Mammalian Expression Topaz-C1 - Mammalian Expression Topaz-pBAD - Bacterial Expression mTopaz 514 527...- Mammalian Expression mTopaz-C1 - Mammalian Expression mTopaz-pBAD - Bacterial Expression moxVenus 514...
  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ...Empty mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag ...localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector ...Species-Specific Expression If you want to drive expression of your favorite gene, you will need a plasmid with...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ... Expression - Rubin lab plasmids for Drosophila expression pMT-puro - Inducible...C-terminal GFP for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 ...
  6. Luciferase Plasmid Collection

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    ...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...a reference level of expression. Browse or search for popular plasmids expressing luciferase. We also ... preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description...Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression Renilla...of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid... into a luciferase-containing plasmid and the effects on gene expression can be measured by the luminescence...
  7. TALEN Plasmids and Kits

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    ...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built... For both plasmids sequence positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting...Maria-Elena Torres-Padilla These plasmids were designed for mammalian expression of fluorescent TAL effectors...pTAL4. The pTAL7 destination plasmids were optimized for TALEN expression in mammalian systems including...TALE Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use ...
  8. Arf GTPase Family

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    Collection
    ... Richard Kahn Lab Plasmids GTPase Plasmids GAP and GEF Plasmids References The ARF (ADP-ribosylation factor...examples of plasmid types available for each gene. Click the gene name for a full list of plasmids available... have plasmids available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs. Plasmid...
  9. Retrovirus Plasmids

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    Collection
    ...Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids Packaging Plasmids Envelope Plasmids Additional...MSCV Plasmid for transgene expression with luciferase expression. Scott Lowe 9044 pMIG MSCV Plasmid for... shRNA expression with GFP expression. See plasmid 8452 for puromycin resistance. William Hahn 63704 pRetroX...for transgene expression with GFP expression. See plasmid 12282 for the addition of WRE, a post-transcriptional...Tet-regulated expression of shRNA. Expresses rtTA. See Zuber et al. (2011) for additional plasmids in this ...pLncEXP CMV/MSV lncRNA expression plasmid. Lei Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in ...Use with packaging plasmid. Bob Weinberg 12259 pMD2.G VSV-G-expressing envelope plasmid. Didier Trono 35617...
  10. Genetic Code Expansion

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    Collection
    ...Find GCE synthetase plasmids, target plasmids, and bacterial strains useful for expanding the genetic... Plasmid Collections Genetic Code Expansion Genetic Code Expansion Synthetase Plasmids Target Plasmids...achieve these modifications, two plasmids are generally used. Once these plasmids have been introduced in the...protein translation machinery. A synthetase plasmid expressing the engineered tRNA and its cognate aaRS ... ncAAs. This plasmid often carries multiple tRNA copies for efficiency. A target plasmid containing the...NIH-funded GCE4All Research Center . Synthetase Plasmids Browse plasmids that contain aminoacyl-tRNA synthetases... of these plasmids also contain one or multiple copies of the cognate tRNA gene. ID Plasmid Synthetase...
  11. Fluorescent Proteins: FRET

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    Collection
    ...backbones and control plasmids. Donor Acceptor λ Dex QY D λ Aem ε A QY A R 0 J(λ) Plasmids mTagBFP sfGFP 399.... Browse Addgene's Luciferase Plasmid Collection or the Promega Plasmid Collection to find related tools...Find plasmids and resources for FRET experiments to measure protein interactions or conformation changes... Plasmid Collections Fluorescent Proteins FRET Fluorescent Proteins: FRET FRET Pairs FRET Calibration...FRET on our blog and explore the tables below for plasmids and information to help plan your next FRET experiment...fluorophores through linkers, fluorescent protein expression level and maturation time, and other environmental...The articles or collections listed below feature plasmids with positive and negative FRET controls or calibration...
Showing: 41 - 51 of 51 results