We narrowed to 324 results for: cloning

Interview with Nicola Patron about her work in plant synthetic biology and her Golden Gate MoClo plasmid kit.

Find out what were Addgene's most popular blog posts in 2020!

Learn about different strategies used to generate plasmids for multiplex gRNA expression and how to use them in various organisms.

An exploration of how the Trimmer lab developed their Addgene-deposited collection of recombinant antibodies.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

REPLACR-mutagenesis is a new in vivo method for mutating your plasmid constructs. Learn how REPLACR stacks up against other mutagenesis techniques.

This blog series showcases some of the new CRISPR tools we have at Addgene. Read about CRISPR-Cas12b for human genome editing, plant base editors, string assembly gRNA cloning, lineage tracing tools, and more.

Choose the best empty backbone for your experiment from some of the most popular in Addgene's library.

Learn about the importance of antibiotic resistance genes in plasmids. How they work, how to use them, and some great tips.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Curious about what it's like to be a summer intern at Addgenie? Addgenie Intern Toj explains it all!

Need help with the plasmid deposit process at Addgene? Consult this post for an in-depth walk through the entire process.

Use the deposit spreadsheet to quickly deposit multiple plasmids with Addgene.

Addgene's plasmid kits allow you to order many plasmids for a single purpose at a low price.

The basics of PCR covering end point PCR, qPCR, ddPCR, and multiplex PCR and how each method is used.

Learn how DNA methylation may affect the activity of certain restriction endonucleases.

Learn a variety of techniques you can use increase the fidelity of multi-part Golden Gate Assembly reactions.

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

Check out the top ten blog posts on the Addgene blog from 2019. Topics include CRISPR, fluorescent protein, plasmids, and more.

Learn about a useful technique to screen phage genomes for host binding proteins and discover new research avenues.