We narrowed to 229 results for: cre encoding plasmid

...pool and extract plasmid DNA directly from the colonies. Transform the resultant plasmid DNA into E. coli...Assuming they expressed well, the proteins encoded by the plasmids in the library will remain attached to ...pinpoint the genes within a sequenced genome that encode for functional cell-binding proteins in the (all-too-common...DNA bases.  Ligate and transform your resultant plasmid libraries into chemically competent E. coli and... you’re using a pET vector) and antibiotic for plasmid selection. Remove air bubbles between the membrane...the original plate to identify which phage gene encodes that protein! First, block your membranes with ...Exploring the interactions between bacteriophage-encoded glycan binding proteins and carbohydrates." Current...

...interest endogenously and transfect them with a plasmid encoding your protein of interest. Then you should ... protein in a Western blot. You acquire a plasmid encoding your protein of interest, known as Gene of...parallel, you transfect cells with another plasmid not encoding this protein, which we will call Negative...blog post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ready-to-use...band from the cells transfected with the other plasmid. Your antibody works! Multiple (Independent) Antibodies...Designing a recombinant validation experiment? Find plasmids here!  References Baker M (2015) Reproducibility...IWGAV five proposed antibody validation pillars. Created with BioRender.com.     Orthogonal Strategies...

...transfer plasmid, a plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from.... coli. In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits.   ...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell...) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression was detected with direct...offline and discarded. Confirmation of the transfer plasmid Addgene uses a rigorous barcode matching system...

...molecular biology reference Find Cre-lox plasmids at Addgene Browse plasmids at Addgene ...cells with an inactive opsin gene; in Cre expressing cells, however, Cre induces the recombination of the ... about FLEx vectors Download our Plasmids 101 eBook Read our plasmids blog posts Resources on the Addgene...(Ernst et al., 2014). Microbial rhodopsins are encoded by opsin genes, and include bacteriorhodopsins,...researchers inject genetically modified viruses encoding microbial rhodopsins into specific cerebral regions...achieved through site-specific recombinases such as Cre or Flp that induce DNA recombination at defined recognition...loxP and lox511) in opposite orientations. Since Cre does not cause recombination between mismatched recognition...

...the Cre-dependent SPH construct for generating the SPH mouse. (B) AAV plasmids used to express CRE under... of Cre-dependent mouse lines for overexpressing genes within discrete cell populations makes Cre-dependent...Constitutive or inducible Cre recombinases can be introduced by AAV or by mating with Cre-expressing mice. ...neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific promoters, including...comprehensive CRISPR guide Find CRISPR plasmids and resources Find CRISPRa plasmids ... sensitive applications such as CRISPR screening. The plasmids used by the Qi Lab for this particular ...genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the protein ...

...the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles...endocytosed, the Cre recombinase payload activates a floxed reporter gene (genetically encoded or delivered...projection neurons. Created with BioRender.com. Addgene has tested these capsid plasmids in vitro and uses...barcoding Addgene’s own AAV6 and AAV11 packaging plasmids Mapping anterograde synaptic connections with ...experiments and see the difference! Find mGold2 plasmids here! Lee, J., Lai, S., et al. (2025). Bright...., 2025). By co-delivering TRV1 with TRV2 that encodes ISYmu1 and an ωRNA under the same promoter, they... to the broad host range of TRV. Find TRV-TnpB plasmids here! Weiss, T., et al. (2025). Viral delivery...

...system is spread across three plasmids, with the response regulator encoded in the genome. To make the system...systems were spread over a number of plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also...et al. condensed the system to two streamlined plasmids. The utility of these TCSs was limited by a few...constructs have been greatly streamlined; only two plasmids are required per system. Applications of these...many strains of E. coli, as well as with other plasmid-based systems, which will enable the assembly of...sb500273n. Epub 2014 Oct 11. PubMed. Find the plasmids from this publication at Addgene. Synthetic biology...2005 Nov 24;438(7067):441-2. PubMed. Find the plasmids from this publication at Addgene. A synthetic...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.        A retroviral...treatment of cardiovascular disease. Find the LipoGlo plasmids at Addgene! Lawler et al, QJM: An International...promoter systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications,...Communications, 2019. PubMed PMID: 31451697. Genetically encoded voltage indicators for use in worms Article contributed...contributed by Brook Pyhtila Genetically encoded voltage indicators (GEVIs) change fluorescence in real time...

...system as an example.  The lentiviral transfer plasmid encodes your insert of interest, flanked by long terminal...of lentiviral particles.  The envelope plasmid typically encodes the vesicular stomatitis virus G protein...integrase-deficient packaging plasmid instead of the standard packaging plasmid. For more details on how to...lentiviral genome is distributed across multiple plasmids. For simplicity, we’ll use the second-generation...this portion into the host genome. The packaging plasmid contains Gag, Pol, Rev, and Tat genes. Specifically...Specifically for our knowledge here, the Pol gene encodes three separate enzymes that are proteolytically processed...into the host cell.  Transfecting these three plasmids into cells enables the production of lentiviral...

...Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids Packaging Plasmids Envelope Plasmids Additional...hTERT for creation of immortalized cells. See plasmid 1771 for puromycin selection, and plasmid 1774 for...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...particles have been split into multiple plasmids for safety: Transfer plasmid — contains transgene, sgRNA, or ...flanked by LTRs Packaging plasmid — contains packaging genes Envelope plasmid — contains packaging gene...Popular Gamma-Retroviral Transfer Plasmids Want a retroviral plasmid with your gene of interest? Search...database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo...

...First, why are plasmids incompatible? Plasmid incompatibility occurs when multiple plasmids within one cell...control plasmid replication of each plasmid using unique replication machinery. However, when plasmids have... one plasmid lost from the cell at a higher rate than the coresident plasmid. Asymmetric plasmid loss ...including one plasmid blocking replication of another and one plasmid outcompeting the other plasmid for replication... copy plasmid and the larger, low copy plasmid is more likely to be lost over time.  Is plasmid incompatibility...develop a plasmid-based tool that is easy to use with other plasmids.  How do you know if your plasmids are ...about plasmid cloning Resources on Addgene.org Download the Plasmids 101 eBook  Browse plasmids at Addgene...

...compatible with single gene KO and pooled screening approaches; (4) encodes a Thy1.1 selectable marker to facilitate...FITS deposit also includes pLX_305_Ovalbumin, a plasmid that enables introduction of the model antigen,...to study antigen-specific responses. Find FITS plasmids here! Marty LaFleur is a Postdoctoral Fellow in... vivo screens in naïve T cells. More broadly, this system can be used to perform in vivo screens in other...power of in vivo screens. Figure 1: Using FITS to perform in vivo CRISPR screens to assess T cell...FITS In vitro CRISPR screens in immune cells have identified many putative regulators of immunity. However...However, in vitro screens cannot faithfully model the complex spatial, cellular, metabolic, and cytokine...

...the MG-2I-dL5** FAP-TAP system works. Find the Plasmids from the Publication Here Light-induced cell ablation...advantages of MG-2I-dL5** over other genetically encoded photosensitizers is that it works with NIR light...2I-dL5** head-to-head with KillerRed, a genetically encoded red fluorescent photosensitizer protein that generates...., . . . Lukyanov, K. A. (2005). A genetically encoded photosensitizer. Nature Biotechnology,24(1), 95...two iodine residues. Compared to MG, MG-2I has increased ROS generation when bound by the dL5** FAP.  The... KillerRed requires mitochondrial targeting to increase cell ablation efficiency, but dL5** phototoxocity...

...Microbial Opsin Plasmids Optical Switch Plasmids Additional Resources Optogenetics plasmids encode proteins ... Plasmid Collections Optogenetics Plasmids Optogenetics Plasmid Collection Microbial...that are controlled by light. Many of these plasmids encode for microbial opsins, which are membrane channels...Microbial Opsin Plasmids Browse, sort, or search the table below for the optogenetics plasmids for neuronal...ready-to-use AAV preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter... top Optical Switch Plasmids Browse, sort, or search the table below for plasmids that use light to control...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Transfer Plasmids Packaging ...used with 3rd generation transfer plasmids along with a plasmid encoding rev . Didier Trono 12253 pRSV-Rev...Packaging Plasmids Envelope Plasmids Resources Lentiviruses, a subset of retroviruses, are some of the most common...(a virion) are divided among multiple plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA...flanked by LTRs Packaging plasmid — contains packaging and regulatory genes; one plasmid in second-generation...second-generation, two plasmids in third-generation Envelope plasmid — contains packaging gene env This collection highlights...Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express a CRISPR gRNA? Browse...

... multiple plasmids. Second generation packaging systems use 3 plasmids; an envelope plasmid that usually...usually encodes VSV-G, a packaging plasmid containing structural genes and a transfer plasmid carrying the...Rev gene from the packaging plasmid and incorporating it into a fourth plasmid. In addition, the promoter...and packaging plasmids in order to obtain the best titers. Requiring an additional plasmid for transfection...transfection and titers) To produce lentivirus, the plasmids encoding the structural elements, envelope, and transfer...dividing the viral genome into additional plasmids thereby increasing the number of recombination events that...By dividing the viral elements across multiple plasmids, the risk of recombination and consequently, the...

...reconstitute the two pieces of the transgene.The AAV encoding part A of the transgene contains a promoter and...transgene, followed by a splice site donor. The AAV encoding part B of the transgene contains a splice site...transduced by both viruses, if the AAV genomes encoding part A and part B of the transgene form a heterodimer...overlapping and trans-splicing methods. The AAV encoding part A of the transgene contains the promoter,...donor sequence and the region of homology. The AAV encoding part B of the transgene contains the region of...therapy Learn about the parts of an AAV transfer plasmid Resources on Addgene.org: Check out the Addgene...plus one additional split vector strategy for increasing the size of the AAV donor template for CRISPR-mediated...

...AAV-pgk-Cre PGK Cre expression Recombinases Patrick Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre...pENN.AAV.CamKII 0.4.Cre.SV40 CamKII Cre expression Recombinases James M. Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato... by using this serotype to deliver Cre-dependent transgenes in Cre transgenic lines. As part of our standard...pAAV-hSyn-DIO-EGFP Syn EGFP, Cre-dependent Control Bryan Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent...CAG tdTomato, Cre-dependent Control Edward Boyden 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control...Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive cells. Control Brandon Harvey...Recombinases Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Karl Deisseroth 55637...

...GPCRs of interest. The TRUPATH plasmid kit consists of 20 plasmids encoding 14 alpha, 2 beta, and 4 gamma...there are approximately 950 different human genes encoding GPCRs (Takeda et al., 2002). G proteins: The receptor...response? Let’s take a look at a few examples with plasmids deposited at Addgene.  PRESTO-TANGO: The ligands...Bryan Roth’s lab also assembled a collection of plasmids to elucidate the role of 14 of the 16 non-visual...pathways mediated by GPCRs? The Kruse Lab constructed plasmids that can be used in peroxidase-catalyzed proximity... (firing or inhibition). AAV preps from DREADD plasmids allow you to easily express a DREADD receptor ...Addgene's chemogenetics guide Find chemogenetics plasmids for your research ...

...protein guide Find plasmids for subcellular localization Find fluorescent protein plasmids ... alternative strategy to develop a genetically encoded antibody based probe that detects the widely used..., Y., … Stasevich, T. J. (2019). A genetically encoded probe for imaging nascent and mature HA-tagged ...fluorescent signals in in vivo imaging: the 10xHA tag increased the fluorescent signal to noise ratio five fold...scaffold and HA-binding regions, the Stasevich lab created a versatile tool that can be used to visualize ...