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We narrowed to 168 results for: crispr library

Showing: 41 - 60 of 168 results

  1. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...(2015) Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach. Nat Methods..., Wang X (2015) WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system. Genome... More about Genome-wide Screens with CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on...Catch Up on Your CRISPR Background with Our CRISPR Guide Pages ... Two ways to use CRISPRa for your research CRISPRa for specific gene targets CRISPR activation for genes...Institute for Biologically Inspired Engineering. CRISPR/Cas9 is an enormously plastic tool and has taken...loci to create changes in phenotypes. For example, CRISPR activation has been used to differentiate induced...

  2. Viral Vectors 101: Viral Applications

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    Blog Post
    ...transfections, preserving cell viability. Library delivery Screening libraries, large collections of biological...transcription factors, barcoded libraries to trace cell lineage, and so much more! Libraries allow you to test thousands...inducibly depending on the vector and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift...increasingly popular tools with many applications. These libraries include sgRNAs targeting the entire genome, overexpression... instead of just one at a time. Many of these libraries are packaged and delivered with viral vectors ... host cell) can be tuned so that the copies of library cargo delivered are controllable. For many screening... is essential to deliver a single copy of your library cargo to isolate the effects of each variable being...

  3. Plasmids 101: NGS Quality Control for Pooled Libraries

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    Blog Post
    ...need to amplify a library for your own experiments. Library amplification 101 Library amplification seems...More about Pooled CRISPR Libraries Resources on Addgene.org Browse Pooled Libraries at Addgene ...plasmid libraries containing hundreds, thousands, or even a million plasmids. These libraries are some...for most libraries. Here are the key points you’ll need to remember when undertaking library amplification...individual plasmid in the library. Bacterial growth must also be optimized to maintain library diversity. If the...other cells. For libraries where the plasmids are all very similar sizes, like a gRNA library, this is less... of the library should be confirmed using next-generation sequencing (NGS). For gRNA libraries, you’ll...

  4. CRISPR-based Genome Editing Systems For Editing in E. coli

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    Blog Post
    ...Addgene blog: Browse CRISPR articles from our CRISPR topic overview Find other CRISPR methods for bacteria...integration using CRISPR-associated transposases” or MUCICAT. Here, we’ll describe each of these CRISPR systems....N-acetylglucosamine strain library construction in 8 days. To build an equivalent strain library, our pEcCas/pEcgRNA... Resources on Addgene.org: Visit the CRISPR Guide Get CRISPR plasmids from Addgene ...Yang lab, we’re continually optimizing the use of CRISPR editing systems in bacteria. Our pCas/pTargetF ...editing), our MUCICAT system which is based on the CRISPR-associated transposase system, INTEGRATE, is best...N-acetylglucosamine biosynthesis, we constructed a strain library with 1-11 copies of integrated genes. We obtained...

  5. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post
    ...functional score based on its library frequency before selection compared to its library frequency after selection...mutational scanning libraries. While each of these methods works well to generate libraries, they all have ...in Your Plasmids Learn How You Can Use CRISPR Pooled Libraries to Generate Mutant Populations Use REPLACER... Use CRISPR Pooled Libraries to Generate Mutants ...technique relies on the generation of a plasmid library that expresses all desired variants of a protein... this process is the ability to generate full libraries of mutant proteins. Researchers from the Whitehead...technique that can be used to generate complex libraries of mutant plasmids ready for deep mutational scanning...

  6. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post
    ...Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn about CRISPR History ...In order to bind DNA, Cas9 and other CRISPR enzymes require a short PAM sequence adjacent to the targeted...encoding phage gene III. The accessory plasmid library contained all 64 possible NNN PAM sequences adjacent...to a protospacer. If ω-dCas9 could recognize a library PAM, it would induce transcription of gene III,...final round of evolution, they used a no-G PAM library to enrich for Cas9 variants with activity on non-NGG...cleavage, they tested the clones against an NNN PAM library in bacteria where cleavage leads to the loss of...Learn about Engineered Cas9 PAM Variants Read about CRISPR Base Editing Learn about Homology Directed Repair...

  7. Hot Plasmids: Summer 2025

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    Blog Post
    ...Addgene has pooled libraries for any summer project. While many barcoding libraries are available for ...RNA Recovery (LARRYv2) barcoding libraries. The team used these libraries to analyze pre-existing heterogeneity... TnpB for plant genome editing LARRYv2 pooled libraries for cell barcoding Addgene’s own AAV6 and AAV11... TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search for smaller...more in our recent blog post on TnpB). Similar to CRISPR-Cas, TnpB is directed to genomic targets using ...9.   Make a splash with new LARRY barcoding libraries By Mike Lacy Whether you’re planning on lounging...(Figure 3). Figure 3: Using LARRYv2 libraries for state-fate analysis in ex vivo HSC expansion...

  8. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...article: CRISPR-based editing in organoid Biosensors for detecting strigolactones The CRISPR Corner New...10.1021/acssynbio.0c00192   The CRISPR corner By Jennifer Tsang New CRISPR plasmids are always being added...To find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...from the past couple months: A new lentiviral CRISPR library from Alec Kimmelman targets mouse metabolic...relative to wild type. A new genome-wide CRISPR knockout library from Xiaole Shirley Liu's lab targets all...system, showing the workflow for inducible CRISPR inhibition (CRISPRi). A) Lentiviral vector design. B) Strategy...New ready-to-use viral preps New toolkit for CRISPR-based genome editing in organoids By Ashley Waldron...

  9. It’s CRISPR Week at Addgene!

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    Blog Post
    ...too! The CRISPR field is always changing so we’ve been busy updating our CRISPR guide. Our CRISPR guide ...for planning your CRISPR experiment. For a quick guide to CRISPR, download our CRISPR Cheat Sheet....you use CRISPR. Hope you will join us this week at #AddgeneCRISPRweek. We updated the CRISPR guide! If..., we received the first CRISPR plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository...That’s a lot of CRISPR! As we’ve shared these tools over the years, the amount of CRISPR educational resources...about CRISPR. We’ll have a different theme for each day. Beginning today, we’ll start with a CRISPR overview...using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications...

  10. Modulate the Activity of 17 Signaling Pathways with One Kit!

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    Blog Post
    ...How the Clon Tracer Library Has Been Used in Cancer Lineage Tracing Read How CRISPR Can Be Used in Genome-Wide...studies in the literature to curate the perfect library, obtained the appropriate cDNAs from other researchers...and attached a DNA barcode to each to make the library usable for pooled screens. Applications of the ... over the world who are interested to use our libraries to do things - sometimes drug screens and sometimes...possible.” The researchers have plans to expand the library to include activators for more cancer-related pathways...Find Other Kits for Your Research Find Pooled Libraries for Your Research View our Hot Plasmid Pages to...

  11. Learning Technical Skills at a Lab Central Workshop

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    Blog Post
    ...Biology Learn about CRISPR Pooled Libraries Resources at Addgene.org Find pooled libraries at Addgene Find...advancement easier. NEBNext Ultra FS DNA Library Prep Kit To create a DNA library, one must first fragment a DNA...NEBNext Ultra II DNA Library Prep Kit for Illumina, one was only able to extract libraries from previously ...by trying out NEB’s new NEBNext Ultra II FS DNA Library Prep Kit for Illumina sequencing and took a class...previously fragmented DNA.The general process for library preparation using this kit is: Fragmentation and end...sizes and also produced high yields of quality libraries from small amounts of DNA i.e 100 pg. At the start...the end of the workshop, all our newly created libraries were analysed to see how well the kit worked. ...

  13. 10 Great Guest Posts We're Thankful For

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    Blog Post
    ...you’re getting started with them. CRISPR Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening...how you can use CombiGEM to barcode small libraries of CRISPR gRNAs to use for screening experiments. Get...Optimizing Donor DNA for Enhanced CRISPR Genome Editing CRISPR has revolutionized how researchers make ... Get the full details on how to make these libraries from the post and the associated publication. Optimizing...and other types of genome edits, but, even with CRISPR, it isn’t always easy to insert a section of DNA...biophysics! Casilio: An Adaptive, Multitasking “CRISPR-OS” In this post, Albert Cheng and Mark Wanner ...

  14. Addgene Welcomes John Doench to the Advisory Board

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    Blog Post
    ...Working with CRISPR Recently Doench has been working on developing CRISPR pooled libraries for use in ...  Resources at Addgene Find CRISPR resources Find CRISPR pooled libraries Learn about other members of...technology at the start of the CRISPR craze, but he says, “In the case of CRISPR, the hype was completely justified...biological process X, you can do with CRISPR technology...” and “with CRISPR, just the results are so much cleaner...understanding, curation, and explanation of our CRISPR resources. We recently sat down with Dr. Doench...and has contributed much of his knowledge to the CRISPR resources at Addgene. His background in history... technology.” Doench thinks that the future of CRISPR will be in high throughput functional characterization...

  15. CRISPR Cheat Sheet

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    Blog Post
    ...Applications of CRISPR: Cut: CRISPR can be used to cut the DNA sequence. With a repair template, CRISPR can be...Jennifer Tsang. We have some crisp new CRISPR material for you!  The CRISPR field moves fast. That’s why we’...Addgene’s CRISPR 101 eBook. This new edition of the eBook includes the details on advances in CRISPR including... Prime editing Anti-CRISPRs … you name it! So get excited and download the CRISPR eBook today! P.S - ...refresher before you dive into the CRISPR eBook, check out the CRISPR Cheat Sheet. Download the PDF using...same information. Download a PDF of CRISPR Cheat Sheet here!   CRISPR Cheat Sheet Clustered Regularly Interspaced...applied goal. Components of CRISPR: gRNAs: Sequences of RNA that direct the CRISPR system to cut other DNA...

  16. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...this method. Step 1: Prepare a random fragment library of phage DNA This first step involves digesting...  Ligate and transform your resultant plasmid libraries into chemically competent E. coli and analyze ...for appropriately sized inserts. We consider a library worth using if at least 40% of colonies tested ... liquid culture, dilute and plate the complete library of transformants for single colonies (this usually...competent strain that you can use for long-term library storage. After overnight growth, scrape up, pool...well, the proteins encoded by the plasmids in the library will remain attached to the membrane at the same... or to any “sticky” proteins expressed by your library. After blocking for 1 h at room temperature with...

  17. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...       The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To...To find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources... very fast CRISPR, is a light-inducible CRISPR system developed by Taekjip Ha’s lab where Cas9-gRNA complex...different antibiotic resistance markers. New CRISPR pooled library from Keith Joung’s and Benjamin Kleinstiver...auxin inducible degron system for C. elegans New CRISPR plasmids New items from the viral service Epigenetic...Saptrap cloning systems to generate AID* tagged CRISPR knock-in repair constructs. These repair constructs...tagged AID* into genomic sites of interests via CRISPR techniques. This tool-kit includes an array of ...

  18. Lentiviral Vector Uses and Overview

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    Blog Post
    ...PMC4273606. 4. Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F...Plasmid FAQs Answered Learn How Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Read...Lentiviral vectors are the vector of choice for many CRISPR applications, and they’ve also had success in clinical...difficult-to-transfect cells. Lentiviral barcoding libraries have been used for complex applications like lineage...transgene-expressing or knockout cell lines. Many CRISPR plasmids are designed for lentiviral use, including...Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out Our...including Addgene’s top requested plasmid in 2015: lentiCRISPR v2, used both for targeted modification and genome-wide...

  19. 2023 and 2024: Reflections and Looking Ahead

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    Blog Post
    ...the human transcriptome. The MORF library is only one of 22 libraries, created from 14 deposits, that became...countries around the world. That included 2008 pooled libraries, 290 antibodies, 18,359 viral items, and 166,247...collection, a comprehensive transcription factor library, in lentiviral vectors. At 3500 plasmids, this ...the recent release of the Spanish version of our CRISPR 101 eBook and the upcoming release of the second...

  20. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

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    Blog Post
    ...gRNA for CRISPR Genome Editing CRISPR-Cas9: Tips for Optimizing sgRNA Activity Lentiviral CRISPR Libraries...tandem. They went on to show that their CRISPR/Cas9-nuclease and CRISPR/Cas9-nickase vectors work successfully...Find the Mulitplex CRISPR/Cas9 Assembly System Kit at Addgene here. Other Popular CRISPR Posts: How to ...established all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering is now...multiplexity is one of the most advantageous properties of CRISPR/Cas9 compared to ZFNs and TALENs,” said Tetsushi...system offers ease and efficiency for all-in-one CRISPR/Cas9 vectors expressing up to seven guide RNAs ... Figure 1: Schematic overview of the all-in-one CRISPR/Cas9 vector construction system for multiplex genome...
Showing: 41 - 60 of 168 results