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We narrowed to 55 results for: dcas9 coding sequence

Showing: 41 - 55 of 55 results

  1. CRISPR Guide

    Type
    Collection
    ...achieved by using orthogonal dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent... be any ~20 nucleotide sequence, provided it meets two conditions: The sequence is unique compared to ...(PAM) The PAM sequence (NGG) serves as a binding signal for Cas9, but the exact sequence depends on which... target, the seed sequence (8–10 bases at the 3′ end of the gRNA targeting sequence) will begin to anneal...matters. Mismatches between the target sequence in the 3′ seed sequence inhibit target cleavage, while mismatches...determined by both the gRNA sequence and the Cas9 enzyme. Ideally, a gRNA targeting sequence will have perfect ... genetic edits. The dCas9 molecule binds to target DNA based on the gRNA sequence without cleavage. This...

  2. Zebrafish Plasmid Collection

    Type
    Collection
    ... enabling CRISPR/dCas9-interference (CRISPRi) modulation of enhancers and non-coding RNAs in zebrafish... for plasmids that contain a zebrafish gene or sequence . The table below highlights plasmids that contain...Zebrafish Genome Project - Access the latest reference sequence assembly for the zebrafish genome. Zebrafish Model...zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally validated in zebrafish...Periklis Pantazis Lab Multibow: digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow -...tagging - Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous... Gavin Wright Lab. Collections of plasmids that encode zebrafish receptors or monoclonal antibodies to...

  3. Lentiviral Prep Service

    Type
    Collection
    ...Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A ...dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A ...resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description...56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 human genes...non-targeting controls. This backbone does not contain dCas9. 57,050 unique sgRNAs targeting 18,901 human genes...non-targeting controls. This backbone does not contain dCas9. 57,011 unique sgRNAs targeting 18,899 human genes...by an RNA guide (gRNA) that harbors targeting sequences. Browse our repository for a list of gRNA plasmids...

  4. CRISPR References and Information

    Type
    Collection
    ...meningitidis Cas9, or Cas12a from your input sequence or list of sequences. Developed by the George Church Lab ...new window) Identifies gRNA target sequences from an input sequence and checks for off-target binding....window) Identifies candidate gRNA target sequences in an input sequence, which can be an accession number, ..., genomic location, pasted nucleotide sequence, or a sequence text file you upload. Currently supports...program that identifies gRNA target sequences from an input sequence, checks for off-target binding and...window) Identification of gRNA target sequences from an input sequence and checks for off-target binding....

  5. Plan Your Experiment

    Type
    Guide
    ...genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can...modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64) Many different...perfect world, your gRNA sequence would be an exact complement to your target sequence with no homologous sites... the desired target sequence, or on-target activity. Two gRNA targeting sequences with 100% homology to... selected target sequence. Additionally, any mismatches between a given gRNA sequence and your genomic...customization Usually paired with a sequencing method Can’t provide specific sequence information alone Digital ...rare edits Many options like RNA-sequencing, Perturb-seq, deep sequencing, etc. qPCR Knockouts Activation...

  6. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can... separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene...target site by homology to a genomic DNA locus and encodes a primer binding site and the desired edits on ...

  7. CRISPR Guide

    Type
    Guide
    ...achieved by using orthogonal dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent... be any ~20 nucleotide sequence, provided it meets two conditions: The sequence is unique compared to ...(PAM) The PAM sequence (NGG) serves as a binding signal for Cas9, but the exact sequence depends on which... target, the seed sequence (8–10 bases at the 3′ end of the gRNA targeting sequence) will begin to anneal...matters. Mismatches between the target sequence in the 3′ seed sequence inhibit target cleavage, while mismatches...determined by both the gRNA sequence and the Cas9 enzyme. Ideally, a gRNA targeting sequence will have perfect ... genetic edits. The dCas9 molecule binds to target DNA based on the gRNA sequence without cleavage. This...

  8. CRISPR Plasmids and Resources

    Type
    Collection
    ...Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...protocols, find software for gRNA design and deep sequencing analysis, discover links to CRISPR forums, and...repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a...libraries for knockout, activation, inhibition, and barcoding experiments. Browse CRISPR Plasmids by Function...

  9. Tetracycline Inducible Expression

    Type
    Collection
    ...Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9-VPR transcriptional activator...pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi...protein (TetR) and operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA). In normal conditions, the...called Ptet) have been designed by combining tet O sequences with elements like the H1 RNA promoter. And, most...or other species by codon optimization of their sequence and eliminating interactions with endogenous factors...Bujard, H., & Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent transcriptional...Imai 155257 Watermelon Pooled Library Lentiviral barcode library for simultaneous tracing of clonal lineages...

  10. Modular Cloning Guide

    Type
    Guide
    ...DNA containing basic parts (promoters, UTRs, coding sequences, terminators, etc.) flanked by fusion sites...additional plasmids including regulatory elements, coding sequences, CRISPR parts GreenGate Cloning System Plant...up to seven) with a Cas9 nuclease, nickase, dCas9, or dCas9-FokI fusion for use in human or mouse systems...DNA module is flanked with a standard overhang sequence at the restriction cut site, multiple modules ...promoter, a 5' UTR, a signal peptide, a protein-coding gene, and a terminator, as in Figure 1). Next, ...zCas9i, a high efficiency intron-optimized SpCas9-coding gene. MoClo CRISPR/Cas Toolkit for Plants Genome... Engineering, TALEN Stephen Ekker 256 plasmids encoding all possible combinations of pFUS_B4 clones for...

  11. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...pooled library and the use of Next-Generation Sequencing (NGS) to confirm library representation. CRISPR... Zhang N/A 15 Varies CRiNCL - Human CRISPRi Non-coding Libraries 86538 — 86550 Inhibition Human Weissman...Human Paired-guide RNA (pgRNA) Library for Long Non-coding RNAs (lncRNAs) 89640 Knockout Human Wei 3rd Varies...Human Mali 3rd 1 74 Perturb-seq Guide Barcodes (GBC) 85968 Barcode Human Weissman 3rd N/A N/A Retroviral...Available for knockout, activation, inhibition, and barcoding. CRISPR... Addgene's collection. Library Type Activation Barcode Knockout Inhibition Base Editing Prime Editing ...

  12. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors... pegRNA directs the Cas9 nickase to the target sequence where it nicks the non-target strand and generates..., if the edited 3’ flap is excised, the target sequence remains unchanged and available as a substrate...nickase to the target site. The longer pegRNA also encodes a primer binding site (PBS) and the desired edits...

  13. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...gRNA(s) to guide the Cas9 protein to your target sequence, as well as design and clone in the homology arms...2A-PuroR cassette, flanked by microhomologous sequences and gRNA target sites. The first deposited PITCh...produces a single plasmid targeting vector that encodes both a guide RNA transcript and a repair template...

  14. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...with a 1.9 kb stuffer sequence that can be removed and replaced with an shRNA sequence of your choice. More...libraries and can be combined with high-throughput sequencing to perform multiplexed analysis of cells pre-...backbones) here. Cloning and expressing new shRNA sequences pLKO (Plasmid #10878) This plasmid has been used...Plasmid Kit Retroviral Barcoding Library (Kit # 1000000097) The Winslow lab barcoding library consists of...#48201) This retroviral vector from the Gage lab encodes GFP and Cre-recombinase. The Cre-lox system is ...and selection marker removal. CRISPR technology pdCas9-humanized (Plasmid #44246) Expression of a catalytically...Cell Virus, MSCV) each with a unique 6 nucleotide barcode. This collection can be used for in vivo phenotypic...

  15. Bacterial Expression Systems

    Type
    Collection
    ...Plasmids Genome Engineering You may also like… Genetic Code Expansion Collection Microbiology Plasmids Collection...interactions between proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression...Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline (aTc) Escherichia...
Showing: 41 - 55 of 55 results