...appropriate promoter sequence and activation of gene expression. In addition to plasmids encoding these synthetic...CRISPR and RNAi have helped researchers alter DNA sequence and RNA expression - but what if you just want...modulation. While catalytically inactive cas9 (e.g. dCas9) has been fused to a variety of protein modules ...from the El-Samad lab recently deposited plasmids encoding synthetic yeast transcription factors that can...factors, the El-Samad lab has deposited plasmids encoding fluorescent reporters and other proteins controlled...

...about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires no additional... mVenus linked to a mitochondrial localization sequence TOM20 (pTriEX Tom20 mVenus BoPD). In dark adapted...plasmids encoding a fusion between cPYP and tgRFP (PLL7.0 tgRFP cPYP), and plasmids that encode the protein...expensive. Thus scientists have turned to genetically encoded voltage indicators. To expand the voltage indicator...

...target site sequence (20bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence. We have found...target site sequence (30bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence. 3. SpCas9 ...target site sequence (21-22bp) and need to be followed on the 3' end by NGRRT or NGRRN PAM sequences. We have... vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2...of CRISPR-associated (Cas) genes as well as non-coding RNA elements capable of programming the specificity...human genome-wide library to activate all known coding isoforms form the RefSeq database (23,430 isoforms... for transient transfection : 61422 : Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the...

...components: A plasmid in the host bacterium expressing dCas9 A plasmid in the host bacterium that expresses gene...editors capable of editing previously uneditable sequences. Four DNA base swaps, so called point mutations...corrects early stop codons within T7 RNAP. A phage encoding the deaminase TadA-7.10 A mutagenesis plasmid ...

...achieved by using orthogonal dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent... be any ~20 nucleotide sequence, provided it meets two conditions: The sequence is unique compared to ...(PAM) The PAM sequence (NGG) serves as a binding signal for Cas9, but the exact sequence depends on which... target, the seed sequence (8–10 bases at the 3′ end of the gRNA targeting sequence) will begin to anneal...matters. Mismatches between the target sequence in the 3′ seed sequence inhibit target cleavage, while mismatches...determined by both the gRNA sequence and the Cas9 enzyme. Ideally, a gRNA targeting sequence will have perfect ... genetic edits. The dCas9 molecule binds to target DNA based on the gRNA sequence without cleavage. This...

... enabling CRISPR/dCas9-interference (CRISPRi) modulation of enhancers and non-coding RNAs in zebrafish... for plasmids that contain a zebrafish gene or sequence . The table below highlights plasmids that contain...Zebrafish Genome Project - Access the latest reference sequence assembly for the zebrafish genome. Zebrafish Model...zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally validated in zebrafish...Periklis Pantazis Lab Multibow: digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow -...tagging - Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous... Gavin Wright Lab. Collections of plasmids that encode zebrafish receptors or monoclonal antibodies to...

...Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A ...dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A ...resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description...56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 human genes...non-targeting controls. This backbone does not contain dCas9. 57,050 unique sgRNAs targeting 18,901 human genes...non-targeting controls. This backbone does not contain dCas9. 57,011 unique sgRNAs targeting 18,899 human genes...by an RNA guide (gRNA) that harbors targeting sequences. Browse our repository for a list of gRNA plasmids...

...of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can... separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene...target site by homology to a genomic DNA locus and encodes a primer binding site and the desired edits on ...

...Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...protocols, find software for gRNA design and deep sequencing analysis, discover links to CRISPR forums, and...repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a...libraries for knockout, activation, inhibition, and barcoding experiments. Browse CRISPR Plasmids by Function...

...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...pooled library and the use of Next-Generation Sequencing (NGS) to confirm library representation. CRISPR... Zhang N/A 15 Varies CRiNCL - Human CRISPRi Non-coding Libraries 86538 — 86550 Inhibition Human Weissman...Human Paired-guide RNA (pgRNA) Library for Long Non-coding RNAs (lncRNAs) 89640 Knockout Human Wei 3rd Varies...Human Mali 3rd 1 74 Perturb-seq Guide Barcodes (GBC) 85968 Barcode Human Weissman 3rd N/A N/A Retroviral...Available for knockout, activation, inhibition, and barcoding. CRISPR... Addgene's collection. Library Type Activation Barcode Knockout Inhibition Base Editing Prime Editing ...

...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors... pegRNA directs the Cas9 nickase to the target sequence where it nicks the non-target strand and generates..., if the edited 3’ flap is excised, the target sequence remains unchanged and available as a substrate...nickase to the target site. The longer pegRNA also encodes a primer binding site (PBS) and the desired edits...

...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...gRNA(s) to guide the Cas9 protein to your target sequence, as well as design and clone in the homology arms...2A-PuroR cassette, flanked by microhomologous sequences and gRNA target sites. The first deposited PITCh...produces a single plasmid targeting vector that encodes both a guide RNA transcript and a repair template...

...with a 1.9 kb stuffer sequence that can be removed and replaced with an shRNA sequence of your choice. More...libraries and can be combined with high-throughput sequencing to perform multiplexed analysis of cells pre-...backbones) here. Cloning and expressing new shRNA sequences pLKO (Plasmid #10878) This plasmid has been used...Plasmid Kit Retroviral Barcoding Library (Kit # 1000000097) The Winslow lab barcoding library consists of...#48201) This retroviral vector from the Gage lab encodes GFP and Cre-recombinase. The Cre-lox system is ...and selection marker removal. CRISPR technology pdCas9-humanized (Plasmid #44246) Expression of a catalytically...Cell Virus, MSCV) each with a unique 6 nucleotide barcode. This collection can be used for in vivo phenotypic...

...Does my transcription factor bind to a particular sequence? Do these two proteins interact? What happens ...localization: Contains a 2x array of the binding sequence for RNA-binding protein, MS2. Can be used in combination...localization: Contains a 2x array of the binding sequence for RNA-binding protein MS2 fused to dsRED. Can...Tac (lactose/IPTG inducible) MBP, 6x His, signal sequence for localization to the periplasm David Waugh ...N-terminal 6x His tag and an MBP tag. Contains a signal sequence to localize the protein of interest to the periplasm...of N and C-terminal epitope tags, localization sequences, and cleavage sites that can be used to express...Assembly Accessory Reagents Kit Various Plasmids Sequence chosen by researcher Beta-galactosidase Keith ...