We narrowed to 812 results for: eng

... DNA polymerase can create overhangs of varying length (typically 10-12 bp) based on a specific sequence...or tag sequences (where appropriate). The primer length is dependent on the T4 Pol "chew back" reaction...

...the amount of DNA used in a ligation based on the length of the DNA to get a proper ratio of 3 available...found on the web. Just enter the concentration, lengths of your insert and vector, and what ratio you want...

...cells. The problem is that the only version of full-length cDNA you can find for YGOI is in a bacterial expression...enzyme buffer (see (Link opens in a new window) New England Biolabs for more information about restriction ...

... Glanville J, Wolf P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018)...molecular definition, and potential for further engineering as compared to polyclonal or monoclonal antibodies...

... Glanville J, Wolf P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018)...

...within tumours via focused ultrasound. Nat Biomed Eng. 2021 Nov;5(11):1336-1347. doi: 10.1038/s41551-021...antibody?  Similarities:  Generated against full length GFP and converted to recombinant form by the NeuroMab...these processes. The Liu and Petsko labs recently engineered hyperfolder YFP (hfYFP), a FP well-suited for...

... Glanville J, Wolf P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018)...empower the scientific community with the tools to engineer them. Given the ease with which scientists can... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched...Monoclonal Antibodies for Clinical Use. In: Antibody Engineering. InTech Additional resources on the Addgene blog...

...should generally have the following properties: Length of 18-24 bases 40-60% G/C content Start and end...

.... to ligate an annealed oligo insert of 50bp in length into a 5kb vector, mix 100ng of the vector with...

...commercially available restriction enzymes, see New England Biolabs' website . Last Upload: May 18, 2018 Protocol...

...their applications in neuroscience. Curr Opin Biomed Eng. 2019. 12:111-117. doi: 10.1016/j.cobme.2019.10.010... as bright as the other GEVI systems that use engineered FPs. Attempts have been made to optimize their...their fluorescence, which ultimately led to the engineering of the second type of these GEVIs: a FP-fused... voltage sensor. FP-rhodopsin GEVIs have been engineered to have kinetics comparable to its one component... transiently and for genomic integration. Lab-engineered viruses will package your genetic cargo in lieu...

...pluripotency and nuclear reprogramming. Adv Biochem Eng Biotechnol, 2009. 114: p. 185-99. PubMed PMID: 19343304...embryonic stem-cell lines from human blastocysts. N Engl J Med, 2004. 350(13): p. 1353-6. PubMed PMID: 14999088...

...extension time can be adjusted according to the length of the target sequence. FAQ How do I design primers...

...cells for 13 days. lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described...

...temperatures, but you may need to increase your primer length and increase the Tm if you are trying to clone ...

...loading control. 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described...

... until fully dissolved. Pro-Tip This step is challenging due to the high viscosity of PEG. Stirring under...

...Quality Control (QC) Pooled libraries can be challenging and expensive to ensure adequate quality, but...

...of mice via adeno-associated viruses. Nat Biomed Eng 4:97–110 . https://doi.org/10.1038/s41551-019-0501...gRNA together (~4.2 kb) into an AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb... little room for additional regulatory elements. Feng Zhang's group previously packaged Cas9 and multiple...enough to package into AAV. At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is the...ADAR2DD(delta984-1090) are approximately 4.1 kb in length, allowing them to be packaged in AAV.   Split ...transgenes might seem daunting, it’s actually a challenge that the field has overcome with split AAVs. In...When a cell is transduced by both AAVs, the full length gene and/or protein is reconstituted. One way to...

...enabling technologies in synthetic biology. J. Biol. Eng. 2013; 7, 13. PubMed PMID: 23663447. PubMed Central...fragments of the chromosome, which was technically challenging. Other homology based technologies We've described...Central PMCID: PMC3874208 Other Resources: New England Biolabs Gibson Assembly Overview Additional Resources...Protocols Gibson Assembly® is licensed to New England Biolabs by Synthetic Genomics, Inc. ...