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Learn about new RNA-targeting Cas13d enzymes that average only 2.8 kb in size, are easy to package in low-capacity vectors, have high on-target and low off-target editing rates.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Learn how you can use plasmids to teach students about functional genomics with your own "Pathways Over Time" project.

Photoactivatable fluorescent proteins can be switched on (and sometimes off) enabling a variety of techniques and applications.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling and cell-cell communication.

Learn how you can identify your transferable skills and better prepare yourself for your next job interview.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

In this episode of the Addgene Podcast, learn all about Joachim Goedart and his work with fluorescent proteins at the University of Amsterdam.

Find some tips and tricks for using CRISPR for gene editing.

Vectors containing the piggyBac transposon are now available at Addgene. Learn how these unique tools can be used for genome engineering!

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.