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We narrowed to 407 results for: expression vector

Showing: 41 - 60 of 407 results

  1. New and Upcoming Viral Vectors - September 2019

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    Blog Post
    ...ubiquitous expression. pAAV-mDlx-NLS-mRuby2 (99130-AAVrg): The Dlx element directs expression in forebrain...directs strong, ubiquitous expression. This is a Cre-dependent switch that expresses nuclear mCherry in the...pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo...pAAV-EF1a-Flpo (55637-AAV1) Viral vectors coming soon! These vectors should be packaged and available ... of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the...entire AAV inventory. Our new AAVs include: EGFP-expressing AAV for serotype testing Calcium sensors and ...

  2. Sleeping Beauty Awakens for Genome Engineering

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    Blog Post
    ... of vectors for the SB transposase system - one set of vectors for robust constitutive expression (pSBbi...gene (red) for expressing the transposase enzyme.2.) The transposase enzyme is expressed (red stars) and...efficiencies of integrating viral vectors. New Sleeping Beauty transposase vectors Even more recently, Eric ...pSBbi derivatives) and one set of vectors for inducible expression (pSBtet derivatives). The constitutive...constitutive and inducible expression vectors were developed into 16 variants with different selection markers...cells’ defense mechanisms. The use of non-viral vectors, like transposons, avoids many, though not all,...transposition; and 2) a transposon containing the gene-expression cassette that can translocate within the genome...

  3. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    Blog Post
    ...transfected with both vectors. Flex vectors are the solution. Flex vectors expressing fluorescent cytoplasmic...concentrations of vectors expressing the required recombinase. The high concentration of FLEx vectors guarantees.... It became possible to design FLEx vectors (Flip-excision vectors) that only allow one inversion event...types in neuronal networks Double inverted vectors expressing light inducible ion channels (plasmid # 20298...cell specific manipulations in vivo, FLEx vectors expressing the light activatable ion channel under the...specific) control of DNA recombination and gene expression can be advanced by the coordinated use of fellow...means to spatiotemporally control FLP and Cre expression have been developed. Read on to learn more about...

  4. Mouse Modeling, Part 1: Genetically Engineered Mice

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    Blog Post
    ... alters or eliminates the expression of a particular gene. Targeting vectors used to create knockout mice...Inducible gene expression Another type of genetically engineered mouse model allows gene expression to be controlled... that regulates Cre expression. The most common drug used to induce the expression of Cre is tamoxifen...of the Esr1 domain that blocks Cre expression. Then, Cre is expressed and Cre-mediated recombination of...Mice can be engineered by inserting a targeting vector containing the gene of interest into a zygote, ..., and mice with conditional or inducible gene expression. Transgenic and knockin mice As the name suggests...microinjection of DNA or infection with a viral vector containing the gene construct can be used to insert...

  5. Important Considerations When Using AAVs

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    Blog Post
    ... have found that expression occurs quickly and results in long and stable expression (up to 9 months in...the onset of gene expression. However, it has yet to be determined what gene expression looks like at 3 ...-associated viral vectors: packaging capacity and the role of rep proteins in vector purity." Human gene...Tool for Gene Expression in Mammals A Match Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional...Future? I knew little about adeno-associated Viral Vectors (AAVs) before starting my graduate program at the...in locomotion. We have relied heavily upon AAV vectors to deliver chemogenetic/optogenetic constructs ...Although they have a small packaging capacity, AAV vectors were suitable for our needs for the following reasons...

  6. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    Blog Post
    ... an episomal vector system that increases reprogramming efficiency through the expression of a shRNA against...RNA virus Episomal vectors- extrachromosomally maintained DNA plasmids that express reprogramming factors...Reprogramming efficiency of PBMCs with episomal vectors Episomal vectors are based on the Epstein-Barr Nuclear ...long-term expression of the reprogramming factors. However, reprogramming of PBMCs by episomal vectors has ...human cells, thus expressing additional EBNA-1 transiently. The transiently expressed EBNA-1 increases ...protein expression from the other episomal plasmids. When used on PBMCs, the pCXLE episomal vector system...common goal of all reprogramming methods is the expression of the reprogramming factors- OCT3/4, SOX2, KLF4...

  7. Hot Plasmids Spring 2024

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    Blog Post
    ... A cis-splicing vector served as positive control (cis-ctrl). v1, vector 1; v2, vector 2; BD, binding ...SARS-CoV-2 research New viral vector preps of Voltron2 voltage sensors AAV vectors for targeting microglia ... offers vectors for CROPseq-multi using selection by Puromycin, Zeocin®, or nuclear expression of BFP2...voltage sensors By Brian O’Neill New viral vector preps expressing the Voltron2 voltage indicator, created...administering these AAVs with a dilute Cre-expressing vector resulted in a sparsely-labeled field of view...high microglia-specific gene expression when incorporated in an AAV vector (Figure 5). Efficiency and specificity...of miR124T was inserted into the vector to silence transgene expression in neurons and other non-microglia...

  8. Multiple Plasmids at a Low Price: Kits from Addgene

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    Blog Post
    ...variety of pExpression vectors) plasmids. The type IIS restriction sites used in these vectors are of low...easier to produce the constructs you need. Expression vectors within this kit contain a variety of promoters... oocytes. The expression vectors additionally contain various combinations of protein tags (His, Avi, ... with the problem of creating vectors for predictable protein expression in bacteria, researchers at the...Process with pCri Cell-free protein expression kit Cell-free expression systems have been improved for both... the pCell-Free vectors, Gateway-compatible backbones that enable cell-free expression of proteins in ...comparison of different cell-free expression systems; the Cell-free expression test kit represents a set of...

  9. The AAVantages of AAV in CRISPR Screens

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    Blog Post
    ...large packaging capacity and stable expression of lentiviral vectors make them an attractive option. However...using different types of AAV. Expression Another advantage of AAV vectors is how the cargo — in this case...multi-plasmid system, where you have one AAV vector dedicated to expressing Cas9, while the other houses the gRNA...However, safety requirements and expression limitations may be too constraining for some experiments, ... particularly in vivo ones. In those cases, AAV vector screens may be AAVantageous!       In vivo, in ...delivery method for in vivo CRISPR screens. AAV vectors can be made at high titers equal to those achieved...improved safety profile, customization, and enhanced expression in many mouse tissues. Safety AAV and lentivirus...

  10. New and Upcoming Viral Vectors - May 2020

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    Blog Post
    ...Lentiviral preps for expressing SARS-CoV-2 proteins ChR2 for targeted expression DREADDs GFP dependent...preps SARS-CoV-2 protein expression The Krogan lab quickly shared plasmids expressing SARS-CoV-2 proteins ...2xStrep-IRES-Puro: Expression of the SARS-CoV-2 ORF3a Lentiviral preps for expressing the E protein (pLVX-EF1alpha-SARS-CoV...for targeted expression. Plasmid 75470 is now available in AAV1 for Flp-dependent expression of ChR2(H134R...GFP expression patterns, thus allowing you to further restrict gene activation to cells expressing both...pAAV-EF1a-Flp-DOG-NW Connie Cepko Viral vectors coming soon! These vectors listed below should be released at... of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  11. Plasmids 101: Protein Expression

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    Blog Post
    ...Gateway pDEST, and pBAD vectors for example. Protein expression from each of these vectors is controlled by ...resulting in different levels of expression from each vector; lower expression may be required if your protein... and cell free expression systems. Overall the general strategy for protein expression consists of transfecting...which expression system to use depends on several factors: The protein you are trying to express How ...system. Mammalian expression systems Mammalian cells are an ideal system for the expression of mammalian proteins...for mammalian expression utilize viral promoters (SV40, CMV, and RSV) for robust expression post-transfection...insect expression systems; baculovirus infected and non-lytic insect cells. Baculovirus expression systems...

  12. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

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    Blog Post
    ...with AAV vectors are common approaches to control transgene expression by restricting expression to genetically...Addgene blog Download the viral vectors 101 eBook Read our viral vector related blog posts Learn more about... more. With the help of adeno-associated viral vectors (AAV), neuroscientists can combine classical genetic... how these changes affect the phenotype. Viral vectors and genetic approaches are continuously refined...designing experiments with AAV: 1. Getting your viral vector into your cell of interest: AAV tropism and route...Manipulate your cell of interest: Cell-type specific expression of molecular tools and sensors Combinations of...genetically defined cell types. Specific transgene expression can be also achieved in nontransgenic animals...

  13. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

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    ...and efficiency for all-in-one CRISPR/Cas9 vectors expressing up to seven guide RNAs (gRNAs). Sakuma said...cloning method to ligate expression cassettes of the gRNAs into a single vector in tandem. They went on...A newly established all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering...systematically established way of making an all-in-one vector for multiplex genome engineering.” Now, they’...Schematic overview of the all-in-one CRISPR/Cas9 vector construction system for multiplex genome engineering...their CRISPR/Cas9-nuclease and CRISPR/Cas9-nickase vectors work successfully to target multiple sites in cultured...it has not yet been demonstrated, they say the vectors are also likely to work in animal embryos based...

  14. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post
    ...your system. Chemogenetic vectors  Expression of retrograde serotype vectors is present at both the injection...-dependent vectors, this can cause challenges for the experimental design as these vectors can exhibit...phenomenon is to use a retrograde recombinase-expressing vector (e.g. Cre or FLP) to transduce the projection...tracing, but often at the cost of toxicity, low expression, or poor scalability.  Scientists at Janelia ...infects neurons, directs high-level transgene expression, and has retrograde functionality to monitor ...site while also activating or inhibiting gene expression, detecting neuronal signaling, or manipulating...AAV into Broca’s area of a mouse specifically expressing Cre in the hippocampus (Fig. 3). The Cre-dependent...

  15. Easing the Protein Purification Process with pCri

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    Blog Post
    ... information, you can then pick an expression vector and expression system. If you find that your protein...If one prefers expression in E. coli, the use of a thioredoxin A (TRX)-containing vector can improve disulfide... al. "The pCri system: a vector collection for recombinant protein expression and purification." PloS ...special E. coli strains designed for protein expression. Cultures are then lysed and the protein of interest...stability and solubility. Determining the best way to express one’s protein of interest can save a lot of time...not fold well, you should screen a few different vectors to see which gives you the most functional protein...pCri also includes plasmids designed for protein expression in systems other than E. coli, specifically the...

  16. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post
    ...blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors 101: Viral...would not be great for a gene therapy vector! For viral vectors, only the protein for reverse transcriptase...components of the vector particle, some of the RT protein gets packaged inside of the vector particle, along...and this is where viral vectors and natural viruses diverge. For the viral vector, its job as a tool is ...transgenic genome will simply express from whatever promoters were included in the vector (or are nearby in the...instead get expression of the sensor, marker, or whatever other tool you chose to express. In the case...seem like an unlikely candidate to use as a viral vector, but retroviruses have been developed into some...

  17. Viral Vectors 101: Pseudotyping

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    Blog Post
    ...lentiviral vector will infect, pseudotyping can help. In pseudotyping, you produce viral vectors using viral...Sandoval S (2017) Pseudotyped Lentiviral Vectors: One Vector, Many Guises. Human Gene Therapy Methods ...To deliver genes using lentiviral vectors, you need an envelope protein on the virus’s surface and a ...virus. How to pseudotype To pseudotype your viral vector, you use the same protocol for generating lentivirus...Sometimes this is due to the cells that the viral vectors were produced in. For example, production of vesicular...virus, HIV-2, and human foamy virus in cell lines expressing galactosyl(alpha1-3)galactosyl (alphaGal) sugars...than viruses produced in cell lines that do not express these sugars. (Takeuchi et al., 1997). The alphaGal...

  18. AAVs in Retinal Gene Therapy

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    Blog Post
    .... At the time, adenoviral vectors were the most commonly used viral vectors for gene delivery, and they...transiently. However new viral vectors capable of providing long-term gene expression in retinal cells - AAV ... better vector for ocular gene therapy: Adeno-Associated Virus (AAV) While other viral vectors such as...immunogenicity and its transient expression. LV results in long term expression in dividing and non-dividing...technician in a lab using adeno-associated viral vectors (AAVs) to treat an inherited retinal degenerative...tolerate the presence of antigens (such as viral vectors) without triggering a destructive inflammatory ...transduce retinal cells, AAV quickly became the vector of choice for ocular gene therapy. AdV and LV ...

  19. Viral Vectors 101: Calcium Sensors

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    Blog Post
    ...blog  Viral Vectors 101: Biosensors Viral Vectors 101: Viral Applications Viral Vectors 101: An Introduction... for neuron-specific expression (hSyn promoter) and pan-mammalian cell expression (CAG promoter). An improved...mammalian cell expression, and compatible with constitutive or Cre-dependent expression. These sensors...jGCaMP7 and jGCaMP8.   Viral vectors So, what do viral vectors have to do with all of these cool ...also already packaged into viral vectors and ready to go. Pro tip: Vectors with ready-to-use AAV preparations...can be more difficult due to variance in sensor expression if you aren’t working with a stably integrated...named CaMPARI2 is available for neuron-specific expression in both zebrafish and mammalian systems. The ...

  20. Your Top Requested Plasmid in 2016!

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    Blog Post
    ...This is a versatile mammalian expression vector that you can use to express both SpCas9 and your gRNA in...this is a versatile mammalian expression vector that you can use to express both SpCas9 and your gRNA of...the top requested plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang...mammalian cell types. Purpose  SpCas9 This plasmid expresses Streptococcus pyogenes (Sp) Cas9 from a Cbh promoter...resistance gene as separate peptides. PX459 This is the vector backbone. This particular backbone can be used ...N-terminus. This tag can be used to test for SpCas9 expression post transfection by western blot. Overall, this...mammalian cell lines. Alternative forms of this vector contain GFP-tagged and un-tagged versions of SpCas9...
Showing: 41 - 60 of 407 results