Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 73 results for: gag pol

Showing: 41 - 60 of 73 results

  1. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...so—can prove invaluable in the pursuit of public engagement. Studying science communication is an equally...had to say. Moreover, this subject allowed me to engage in findings relevant to my background (neuroimaging...sport athletes, as well as the ethical and health policy implications arising from sport-related concussion...

  2. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...competitive skill sets in a myriad of professions is to engage in extracurricular activities (EA). EAs can: Introduce...expands your professional network. No brainer! Engaging audiences from across the hall, fields, and other...editing manuscripts? Are you interested in scientific policy? Next, use the resources below to identify potential...

  3. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...with him.  He is also available for speaking engagements as are many of the coaches I have mentioned. ...PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet chats,...Consulting works remotely, will travel for speaking engagements and has many areas of specialty from leadership...

  4. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ... ) fwd ITR primer, 5'-GGAACCCCTAGTGATGGAGTT rev ITR primer, 5'-CGGCCTCAGTGAGCGA ITR-containing plasmid... master mix which contains a high-quality DNA polymerase and a blend of dTTP/dUTP to minimize carryover...

  5. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...biochemistry, microbiology, epidemiology, and bioanthropology. Studying human health science made me happy...dissonance (Kunda, 1990). More specifically, they were engaging in a behaviour called the “biased evaluation of...greater science literacy and education have more polarized beliefs on controversial science topics. Proceedings..., L. L., Braman, D., & Mandel, G. (2012). The polarizing impact of science literacy and numeracy on perceived...

  6. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...enter the cell with the concentration gradient to depolarize it (Nagel et al., 2003). Inhibitory opsins are...Na+) against the concentration gradient and hyperpolarize the cell (Mattis et al., 2012). Bicistronic ...the receptor, when exposed to light, the ligand engages the binding pocket and activates the receptor. ...considering for your experiment, note that you cannot extrapolate results from one cell type to another, as they... A. K., Hegemann, P., & Wiegert, J. S. (2021). BiPOLES is an optogenetic tool developed for bidirectional...

  7. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...amazing postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School and a long list of supporters...message delivered by many of the speakers.  As Dr. Polka said as she started things off, “We are all capable... energy and to choose institutions with allies engaged in training excellence to begin effecting change...

  8. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ... the successes of the team. A strong sense of engagement in the work Behind-the-scenes support from their...include training or teaching others, research, and policy development. Every worker at every level does better...all times when making decisions, assignments and polices. Sometimes the boss has to be the boss and make...

  9. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...not just wait for papers to be published, but to engage in ongoing, real-time dissemination of information...No transformation techniques are available yet. Polistes (Amy Toth) These wasps are like the ones that ...Crepidula atrasolea juvenile, light microscope with polarizing filter. Development is direct in this species...actually silence this gene with RNAi to make a polygamous species monogamous. Anolis sagrei (Doug Menke...

  10. The time and cost required to make a plasmid

    Type
    Blog Post
    ...plasmid? Figure 2: Survey results from a Twitter poll run in 2023, n=194.   After the great Plasmid...much harder to calculate, in both our 2018 and 2023 poll, most people said it took them more than 22 hours...these rough estimates, it’s clear that community engagement with and use of repositories like Addgene provides...

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...to express the transcription factors from a polycistronic cassette. However, it was unknown whether variations...variations in gene expression from these polycistronic cassettes could lead to comparable experimental...Woltjen Lab compared different variants of the polycistronic cassettes and discovered reprogramming discrepancies...variation in the Klf4 isoform (KLF4s or KLF4L). Polycistronic cassettes that contained KLF4s had overall diminished...reprogramming and global gene expression patterns. Polycistronic cassettes that contained the KLF4L isoform had...the observed reprogramming discrepancies. The polycistronic piggyBac transposon vectors tested in this work...activity than BE3  85172  pBK-EQR-BE3  EQR-Cas9 (NGAG)  Lower off-target activity than BE3  85173  pBK-VRER-BE3...

  12. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    .... This made sure I didn’t zone out and kept me engaged throughout the day. After the conference If there...conference If the conference has a social media policy, this should be on their website or the program...program booklet. Make sure you’re familiar with the policies before posting something on social media. If someone...

  13. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...realized I wanted a career in science outreach: engaging the public to promote an awareness and understanding... myidp.sciencecareers.org mentioned museums. Indianapolis is home to one of "The world’s biggest and best...children’s museum", The Children's Museum of Indianapolis. I drove passed these dinosaurs every day. They...

  14. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...relevant parties, and the company at large, by polling and informal communications we made a decision ...scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (multiple choice/binary...scientists of all types to stay up to date and engaged with new advances in published science. They also...

  15. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...to solve a problem. But how do you intentionally engage in a creative problem-solving process?  You may...for your idea. Our product team has implemented a policy of never rejecting an idea simply because it's ...innovative group. Our entire company benefits from their policy! Perfectionism When we reach for perfection, our...

  17. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ... your experiment is definitely not a fluke.” 3. Engage in various projects and help colleagues with their...expertise in law, business, journalism, entertainment, politics, K-12, etc.” Addgene's team includes many PhD ...

  19. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...be other scientists, or science journalists, or policy makers. The term editor covers a wide variety of...the Advancement of Science. She is enjoys broad engagement through science communication with a variety ...
Showing: 41 - 60 of 73 results