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We narrowed to 54 results for: golden gate

Showing: 41 - 54 of 54 results

  1. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...Strains Plasmids 101: TOPO Cloning Plasmids 101: Golden Gate Cloning Read all of our Plasmid Cloning Posts...recombinant plasmid to propagate. Any cells that contained non-recombinant vectors (re-ligated empty vectors, ...ccdB aspect, Gateway takes advantage of the same principle that cells will not propagate while expressing...recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as well as several others...focus on a unique gene that makes the popular GatewayTM method possible: ccdB. But what is ccdB, what ...expressed the ccdB gene and were therefore unable to propagate because CcdA wasn’t available to counteract the... to thoroughly screen colonies for the insert. Gateway® technology (developed by InvitrogenTM) is essentially...

  2. Modular Cloning Guide

    Type
    Guide
    ...use with the Golden Gate TALEN and TAL Effector Kit (Voytas and Bogdanove). Platinum Gate TALEN Kit Genome... for Golden Gate assembly of 15-16 RVD TALEN inserts in one cloning step rather than two. Golden GATEway...Wittbrodt A toolkit that combines Golden Gate and Multisite Gateway cloning. This system was specifically...Cloning Applications and Kits Blog: Plasmids 101 Golden Gate Cloning Synthetic Biology Plasmids Modular Cloning... (Weber et al., 2011). This method, based on Golden Gate plasmid assembly, exploits the ability of Type...eight transcription units. Yarrowia lipolytica Golden Gate tool kit Yeast Expression Tristan Rossignol 64...for individual subunits are first assembled by Golden Gate cloning and then combined by Gibson Assembly ...

  3. MXS Chaining

    Type
    Blog Post
    ... Method Technology Pros Cons References Golden Gate Type II restriction enzymes Can assemble up to...

  4. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...test different ideas. We are a huge fan of the Golden Gate based MoClo system. The vectors mentioned in ...expression method enables us to quickly screen and investigate transcription factor candidates and the regulation...

  5. Cloning

    Type
    Guide
    ...Top Type IIS Assembly (Golden Gate & MoClo) Type IIS systems, such as Golden Gate and Modular Cloning (...to use MoClo and Golden Gate cloning kits for your next cloning project. Golden Gate Cloning (Image from...from Plasmid 101: Golden Gate Cloning ) Back to Top Ligation Independent Cloning Ligation Independent ...Back to Top Gateway® Recombination Cloning Gateway cloning (Image from Plasmid 101: Gateway Cloning ) Gateway... Gateway® cloning is a recombination based cloning method. The benefit of Gateway® is that moving a piece...cassettes). Although gateway cloning is a simple and efficient cloning method, gateway vectors and recombination...Guide Genome Engineering Guide Restriction Enzyme Gateway TOPO Gibson Type IIS Ligation Independent Cloning...

  6. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...Blog: Learn How to Verify Your Plasmid Try Out Golden Gate or SLIC Cloning Read Other Plasmids 101 Posts...as this will be useful for the ligation step. 3. Ligate Your Insert into Your Vector In the ligation step...

  8. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...allows assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial micro RNA and short hairpin... of CRISPR technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi..., gene knockout strategies cannot be used to investigate essential genes. Historically, gene silencing... which allows easy creation of long hpRNA with Gateway-based cloning and straightforward positive selection...from Worcester Polytechnic Institute where he investigated how plants achieve polarized cell growth in ...

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...engineering. These components are provided in Golden Gate compatible backbones that can be combined with...all the latest hot plasmid updates!     Tol2 Gateway-compatiable toolbox for studying the nervous system...system The Cole lab recently deposited the Tol2 Gateway-Compatible Toolobox, which will allow scientists...neurobiology and neuorodgenerative diseases. The Tol2 Gateway-Compatible Toolbox is based on the original Tol2kit...empty vectors that have standard cloning sites or gateway compatible cloning sites for easy cloning of your...toolbox adds new neuronal tools to the expanding gateway compatible vectors currently shared amongst the...2007. PubMed PMID: 17937395 Listen to Our Tol2 Gateway-Compatible Toolbox Podcast Segment The impact of...

  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... John W. Campbell. This period is termed as the golden age of SciFi, but the common themes that these ...between Cas9 and reverse transcriptase. Using an elongated template + guide RNA called pegRNA (prime editing...

  13. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...a microscopist, a biostatistician, and that one golden-handed technician who can do a certain difficult...someone to coordinate, someone to decide, someone to delegate, etc. If you find that there is not someone (or...

  14. Luciferase Plasmid Collection

    Type
    Collection
    ...Insertion of 5' promoter/enhancer regions using Gateway cloning. Jorge Ferrer 16539 pBV-Luc Firefly Insertion...Bartel 101139 pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF) Firefly, Renilla Creating...® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2-ccdB-Nanoluc...® Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2...® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X...® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 106253 pLS-mP-Luc Firefly...split-luciferase complementation assay in plants using Gateway cloning. Use with 174051 Alberto Macho 174051 pGWB-cLUC...
Showing: 41 - 54 of 54 results