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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 44 results for: golden gate assembly

Showing: 41 - 44 of 44 results

  1. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...update how we make and distribute kits, I helped assemble a crack team of Addgenies that includes: Michelle...particular function. Project team A Project Team is assembled to complete a series of tasks with precisely defined...a microscopist, a biostatistician, and that one golden-handed technician who can do a certain difficult...project indeed. Mission team A Mission Team is assembled to make progress towards open-ended goals whose...attacking this problem?”, try thinking “Who can I assemble to attack this problem from every angle?” Your...someone to coordinate, someone to decide, someone to delegate, etc. If you find that there is not someone (or...

  3. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ... 1-2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial micro... of CRISPR technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi..., gene knockout strategies cannot be used to investigate essential genes. Historically, gene silencing... which allows easy creation of long hpRNA with Gateway-based cloning and straightforward positive selection...from Worcester Polytechnic Institute where he investigated how plants achieve polarized cell growth in ...

  4. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...concentration of 1 μM). Ligate annealed oligos into pX330 using a Golden Gate assembly cloning strategy 3 ...utilized to be able to perform PCR in step 6.3 . Assemble a 20 μl PCR with the following components: 10 ...
Showing: 41 - 44 of 44 results