We narrowed to 167 results for: ipp
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TypeBlog Post... anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged 14 days post-transfection... the screen to understand mouse cortical and hippocampal neural microcircuits. “In the longer term, this...
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Celebrating 15 Years of Scientific Sharing
TypeBlog Post... despite the evolving export/import rules. Our shipping and lab teams continue to keep up with the demand...have confidence that from Business Development to Shipping we will execute any new projects with our usual... -
Addgene's Tips for Plasmid Quality Control
TypeBlog Post... and reverse reactions, respectively, and then shipped along with their corresponding glycerol plate on...volume and concentration, and not all providers are equipped to perform Sanger sequencing from glycerol stocks... -
Plasmids 101: Cre-lox
TypeBlog Post...conceptually similar to Cre-lox, with flippase (Flp) and the short flippase recognition target (FRT) site being... -
DNA Service - Cloning Grade DNA
TypeCollection...including the check out and will require separate shipping charges. Where can I find cloning resources or... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post... from their assay to Addgene. Drew Endy’s and Philippa Marrack’s labs also deposited plasmids for producing...Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS (2020... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...all of your plasmid data, we’ll send you prepaid shipping materials (i.e., a deposit kit) with instructions...we need to know your Addgene account username. Shipping address and phone number. Plasmid sequences or... -
Common Injection Routes in Mice
TypeBlog Post...Restraint: scruffing Location: throat Gauge (bulb-tipped feeding tube): 18–20 Volume: up to 10 ml/kg Skill...Beginner Oral gavage Scruffing Throat 18–20 (bulb tipped) up to 10 mL/kg Intermediate Resources and... -
DNA Purification Without a Kit
TypeBlog Post...and ball bearings. Then a cellulose dipstick is dipped into this mixture to absorb the nucleic acids. ..., purified total nucleic acids are released by dipping the cellulose directly into a PCR buffer, where... -
Tips for arabidopsis transformation
TypeBlog Post... Growing the transformed agrobacterium culture Dipping your plant’s flowers in the agrobacterium culture...resuspend them in transformation media. If you are dipping your plants, put the culture into a container that... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post... editing enzyme (“Editase”), ADAR2, into the hippocampus of a Rett Syndrome mouse model using an adeno-associated...RNA in each of three neuronal subtypes of the hippocampus. This technology has formed the basis of the ... -
Viral Vector Guides and Plasmids
TypeCollection...viral vectors, wild-type viral genomes have been stripped of any unnecessary accessory genes and divided... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...performed the following modifications to pDS132 (Philippe et al., 2004): Replaced the sacB cassette with...PMID: 29760772. PubMed Central PMCID: PMC5946454. Philippe, Nadège, et al. "Improvement of pCVD442, a suicide... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post... the Clevers lab developed the Confetti mouse (Snippert et al., 2010), a reporter mouse that can be used...PMID: 30454553. PubMed Central PMCID: PMC6245733. Snippert, Hugo J., et al. "Intestinal crypt homeostasis... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post..., including R26R-Confetti described in Hugo J. Snippert, et al. (2010) and the MAGIC Marker strategy described...between symmetrically dividing Lgr5 stem cells. Snippert HJ, van der Flier LG, Sato T, van Es JH, van den... -
mTOR Pathway
TypeCollection... B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von Hippel-Lindau tumor suppressor Return to top mTORC2 Complex... -
Promega Plasmid Collection
TypeCollection...Orders of the 20 µg DNA size are processed and shipped within 6–8 business days. For more information ... -
Distribution to Industry
TypeCollection...MTA documents, your order will be processed and shipped. Industry Distribution Policy A portion of Addgene's... -
Important Considerations When Using AAVs
TypeBlog Post...Serotype Striatum AAV5>AAV9>AAV8>AAV1>AAV6>AAV2 Hippocampus AAV9>AAV5>AAV8>AAV1>AAV6>AAV2 Auditory Cortex...upon which section you’re looking at (striatum, hippocampus and cortex) within the mouse brain (4). An example... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...Principal Investigator (PI), provide us with your shipping address for deposit materials, and agree to our...prepare your plasmids and a prepaid label with shipping materials to send your plasmids to us. Essentially...