...cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline...conditional allele, or floxed mouse, from Yang et al. requires two gRNAs targeting two intronic regions and two...allele The design for a floxed conditional allele requires you to target 2 loxP sites to intronic regions...Overall, the main drawback of i-GONAD is that it requires a high-concentration, column-purified DNA template...

...channelrhodopsin-2, ChR2) fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing cells. The resulting...membrane, a threshold is reached and the neuron fires, sending the electrical signal down the axon (Lodish...

... the Cbh promoter with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion...Otx2 expression in the developing mouse retina requires a unique enhancer and either Ascl1 or Neurog2 ...

...with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian cells. You should also express...Abhishek Chatterjee 220800 EF1a-L274GMmMetRS-T2A-mCherry MetRS M. musculus azidonorleucine (Anl) Mammalian...182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA Y306A/Y384F (AF) pyrrolysine (Pyl) tRNA...