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We narrowed to 206 results for: kars;

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Showing: 41 - 60 of 206 results

  1. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ... at Addgene. Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo... B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der...

  2. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ... S, Joung J, Slaymaker IM, Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev...10.1261/rna.054098.115 Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin... B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der...

  3. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... 2843425.Takara Bio. Tet-On and Tet-Off systems: Second generation. https://www.takarabio.com/products... the system have been engineered and improved. Takara’s “2nd generation Tet-Off vectors (Tet-Advanced)...

  4. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Optogenetics Karl Deisseroth AV-1-26966P 26966-AAV1 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26968P 26968-AAV1 pAAV-Ef1a-DIO ChETA-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26971P 26971-AAV1 pAAV-CaMKIIa-eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26975 26975-AAV1 pAAV-CaMKIIa-hChR2(H134R)-mCherry Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26966P 26966-AAV5 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26969P 26969-AAV5 pAAV-CaMKIIa-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26973P 26973-AAV5 pAAV-hSyn-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth...

  5. Optogenetics AAV Preps

    Type
    Collection
    ...dependent 8 Karl Deisseroth 137144 pAAV-nEF-Coff/Fon-ChR2-mCherry nEF ChR2 mCherry Flp dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 26968 pAAV-Ef1a-DIO ChETA-EYFP EF1a ChETA EYFP Cre dependent 1, 5, 9 Karl Deisseroth...rg* Karl Deisseroth 26971 pAAV-CaMKIIa-eNpHR 3.0-EYFP CaMKII eNpHR 3.0 EYFP Constitutive 1, 9 Karl Deisseroth...Constitutive 2, 5 Karl Deisseroth 137151 pAAV-nEF-NpHR3.3-EYFP nEF NpHR 3.3 EYFP Constitutive 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137154 pAAV-nEF-Coff/Fon-NpHR3.3-EYFP nEF NpHR 3.3 EYFP Flp dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137156 pAAV-nEF-Con/Foff 2.0-iC++-EYFP nEF iC++ EYFP Cre dependent 8 Karl Deisseroth...pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Karel Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 Dlx...

  6. Control AAV Preps

    Type
    Collection
    ...PHP.eB Karl Deisseroth 114472 pAAV-hSyn-mCherry hSyn mCherry Constitutive 1, 2, 5, 8, 9, rg* Karl Deisseroth...Constitutive 8 Karl Deisseroth 131001 pAAV-hSyn-mScarlet Syn mScarlet Constitutive 1, 8 Karl Deisseroth 135630...dependent 9 Karl Deisseroth 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP Cre dependent 1, 2, 5, 9, rg* Karl Deisseroth...dependent 8 Karl Deisseroth 137133 pAAV-Ef1a-Con/Foff 2.0-mCherry EF1a mCherry Cre dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137162 pAAV-Ef1a-Con/Foff 2.0-EYFP EF1a EYFP Cre dependent 8 Karl Deisseroth..., rg* Karl Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP Flp dependent 1, 2, 5, 8, 9, rg* Karl Deisseroth...dependent 8 Karl Deisseroth 137134 pAAV-Ef1a-Coff/Fon-mCherry EF1a mCherry Flp dependent 8 Karl Deisseroth...

  7. Retrograde AAV viral preps

    Type
    Collection
    ...mCherry Control Karl Deisseroth 114469 pAAV-CaMKIIa-mCherry CamKII mCherry Control Karl Deisseroth 50459...Cre-dependent Control Karl Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP, Flp-dependent Control Karl Deisseroth ...Control Karl Deisseroth 114471 pAAV-Ef1a-fDIO mCherry EF1a mCherry, Flp-dependent Control Karl Deisseroth... Recombinases Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Karl Deisseroth 55637...Recombinases Karl Deisseroth 55638 pAAV-EF1a-vCre EF1a VCre expression Recombinases Karl Deisseroth 87306...Optogenetics Karl Deisseroth 26973 pAAV-hSyn-hChR2(H134R)-EYFP Syn Activator Optogenetics Karl Deisseroth...Optogenetics Karel Svoboda 26975 pAAV-CaMKIIa-hChR2(H134R)-mCherry CamKII Activator Optogenetics Karl Deisseroth...

  8. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...found that La, which is ubiquitously expressed in eukaryotes, binds and stabilizes the 3′ tail of pegRNAs .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson, J. W., Newby...s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...

  9. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...An overview of CRISPR's development from a prokaryotic immune system to a valuable genome engineering...revolution shows no signs of slowing down. This prokaryotic immune system is especially amenable to genome...offering flexibility and easy multiplexing/scaling. Prokaryotes have long utilized CRISPR as a powerful defensive...elucidated until 2007. Scientists hypothesized that prokaryotes used CRISPR as part of an adaptive immune system...PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16...system seems like a winning strategy to ensure prokaryotic survival, but it is not invincible. Bondy-Denomy...provides acquired resistance against viruses in prokaryotes. Science . 315(5819):1709-12. PMID: 17379808 ...

  10. Biosensor AAV Preps

    Type
    Collection
    ...5, 8 Karl Deisseroth 137123 pAAV-Ef1a-Con/Foff 2.0-GCaMP6F EF1a GCaMP6f none Cre dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137120 pAAV-Ef1a-Con/Foff 2.0-GCaMP6M EF1a GCaMP6m none Cre dependent 8 Karl Deisseroth...Constitutive 8 Karl Deisseroth 137126 pAAV-Ef1a-Con/Fon-sRGECO Ef1a sRGECO none Cre and Flp dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137128 pAAV-Ef1a-Coff/Fon-sRGECO Ef1a sRGECO none Flp dependent 8 Karl Deisseroth...pAAV-Ef1a-Coff/Fon-GCaMP6F EF1a GCaMP6f none Flp dependent 8 Karl Deisseroth 158757 AAV-CAG-SomaGCaMP6f2 CAG soma-...6m-p2A-ChRmine-Kv2.1-WPRE Syn GCaMP6m none Constitutive 8 Karl Deisseroth 137119 pAAV-EF1a-Con/Fon-GCaMP6M EF1a...pAAV-Ef1a-Coff/Fon-GCaMP6M EF1a GCaMP6m none Flp dependent 8 Karl Deisseroth 137165 pAAV-Ef1a-Con/Fon/Von GCaMP 6m...

  12. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...useful. But they were barely effective at editing eukaryotic cells — at least, until they met the directed...doi.org/10.1038/s41586-019-1323-z Peters, J. E., Makarova, K. S., Shmakov, S., & Koonin, E. V. (2017). Recruitment...

  13. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ... pAAV-Ef1a-fDIO EYFP AAV5. AAV8, AAVrg Control Karl Deisseroth New serotype (multiple) rAAV-CAG...pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE AAV8 Optogenetics Karl Deisseroth New viral prep pAAV-CaMKIIa(0.4)-eOPN3...

  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...OPTN His T7 ALS Jon Ashwell 23376 pDONR223-PRKAR1B PRKAR1B Dementia and/or parkinsonism William Hahn 23452...Parkinson's, FTD Karen Ashe 46905 pRK5-EGFP-Tau AP MAPT GFP CMV Parkinson's, FTD Karen Ashe 46906 pRK5-...Parkinson's, FTD Karen Ashe 46907 pRK5-EGFP-Tau E14 MAPT GFP CMV Parkinson's, FTD Karen Ashe 46908 pRK5...Parkinson's, FTD Karen Ashe 46909 pRK5-EGFP-Tau E14 P301L MAPT GFP CMV Parkinson's, FTD Karen Ashe 46924 YFP-Parkin...-Spatz disease David Root 76838 PRKAR1B gRNA (BRDN0001146606) PRKAR1B hU6 Dementia and/or parkinsonism...parkinsonism David Root 76839 PRKAR1B gRNA (BRDN0001144765) PRKAR1B hU6 Dementia and/or parkinsonism David Root ...Root 76840 PRKAR1B gRNA (BRDN0001147089) PRKAR1B hU6 Dementia and/or parkinsonism David Root 76898 PRKCG ...

  15. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving database, and...Structural Biology. https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf, Y. I., Iranzo, J., et al. (2019)...

  16. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...architecture to every diagram portraying some aspect of eukaryotic DNA would become busy and potentially detract...me at the Keystone Chromatin meeting, especially Karen Reddy (@theReddyLab), Thoru Pederson, Joanna Wysocka...

  18. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...Why do scientists use yeast vectors? Yeast are eukaryotes and thus contain complex internal cell structures...technical advantages that come with working with prokaryotes. This includes but is not limited to: rapid growth...

  19. Recombinases AAV Preps

    Type
    Collection
    ... a fusion tag) 8, rg* Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a none 11, rg* Karl Deisseroth 121675 pAAV-EF1a-fDIO-Cre... fusion tag) 1, rg* Karl Deisseroth 55637 pAAV-EF1a-Flpo EF1a none 1, 2, rg* Karl Deisseroth 87306 AAV...Viral Tools 183535 pAAV-CaMKIIa-Flpo CaMKIIa none 8 Karl Deisseroth 236288 pAAV.TBG.PI.FLPo.rBG TBG none ...Serotype(s) PI 55638 pAAV-EF1a-vCre EF1a none 8, rg* Karl Deisseroth *AAVrg = retrograde serotype, produced...produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory...
Showing: 41 - 60 of 206 results