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  1. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...codon optimize genes from eukaryotic organisms for expression in prokaryotic systems or yeast (Lanza et...base-pairing mechanism for translation initiation in eukaryotes. Nat Struct Mol Biol 13:30–34 . https://doi.org...

  2. Plasmids 101: Repressible Promoters

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    Blog Post
    ... 2843425.Takara Bio. Tet-On and Tet-Off systems: second generation. https://www.takarabio.com/products... the system have been engineered and improved. Takara’s “2nd generation Tet-Off vectors (Tet-Advanced)...

  3. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post
    ...Pancreas AAV6, AAV8 Wang et al., 2006 Retina 7M8 Dalkara et al., 2013 Skeletal muscle MyoAAV Tabebordbar...1359–1368. https://doi.org/10.1089/hum.2008.123 Dalkara, D., Byrne, L. C., Klimczak, R. R., Visel, M., ...Hantman, A. W., Looger, L. L., Schaffer, D. V., & Karpova, A. Y. (2016). A Designer AAV Variant Permits Efficient...

  4. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...found that La, which is ubiquitously expressed in eukaryotes, binds and stabilizes the 3′ tail of pegRNAs .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson, J. W., Newby...s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...

  6. New Viral Vectors - Winter 2025

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    Blog Post
    ... pAAV-Ef1a-fDIO EYFP AAV5. AAV8, AAVrg Control Karl Deisseroth New serotype (multiple) rAAV-CAG...pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE AAV8 Optogenetics Karl Deisseroth New viral prep pAAV-CaMKIIa(0.4)-eOPN3...

  7. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post
    ...and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving database, and...Structural Biology. https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf, Y. I., Iranzo, J., et al. (2019)...

  8. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post
    ...architecture to every diagram portraying some aspect of eukaryotic DNA would become busy and potentially detract...me at the Keystone Chromatin meeting, especially Karen Reddy (@theReddyLab), Thoru Pederson, Joanna Wysocka...

  10. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...Why do scientists use yeast vectors? Yeast are eukaryotes and thus contain complex internal cell structures...technical advantages that come with working with prokaryotes. This includes but is not limited to: rapid growth...

  12. Design Tips for Prime Editing

    Type
    Blog Post
    .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson, J. W., Newby...j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...

  14. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der...plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, ...

  17. Plasmids 101: Protein Expression

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    Blog Post
    ...Insect cells can also be used to produce complex eukaryotic proteins with the correct post-translational ...system to generate large quantities of recombinant eukaryotic proteins. Although many species of yeast can ...

  20. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b...
Showing: 41 - 60 of 204 results