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We narrowed to 119 results for: lenti crispr plasmid

Showing: 41 - 60 of 119 results

  1. Hot Plasmids - January 2023

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    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ...Figure 3 Colognori et al 2022.   Find CRISPR Csm plasmids here! Colognori D, Trinidad M, Doudna JA.... in this post: CRISPR-Csm: A novel RNA targeting system Heat-inducible lentiviral Car constructs Anti-GFP...inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the activity... missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...N86/8R] ready-to-use antibody Hyperfolder YFP CRISPR-Csm: A novel RNA targeting system by: Susanna Stroik... a leg up on the alternatives - RNAi and Cas13. CRISPR-Csm doesn’t degrade proximal RNAs nor does it have...

  2. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post
    ...our comprehensive CRISPR guide Find CRISPR plasmids and resources Find CRISPRa plasmids ...blog Browse our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR eBook Resources... for sensitive applications such as CRISPR screening. The plasmids used by the Qi Lab for this particular...recent papers describe the development of CRISPR activation (CRISPRa) tools for neuroscience, including transgenic...neuroscience research. What is CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead variant... using CRISPRa to target individual genes can be relatively laborious because multiple plasmids/viruses...construct for generating the SPH mouse. (B) AAV plasmids used to express CRE under neuron-specific promoters...

  3. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post
    ...for “CRISPR” plasmids keeps more than half of these. Instead we can navigate first to the CRISPR Resources...300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed... best plasmid for your research can be a daunting challenge. With over 80,000 deposited plasmids from ...educational resources: plasmid, pooled library, blog posts, protocols, and more. Selecting crispr in Guides will...through to the plasmid search results: More than 200 different GCaMP containing plasmids are available...search tool lets you access plasmids from journal articles. From paper to plasmid Authors regularly indicate... to include specific plasmid ID numbers. If you know the ID number for a plasmid, your straightforward...

  4. Top Requested Plasmid of 2017 - pMD2.G

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    Blog Post
    ...discovery. CRISPR is a great example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids), but...requested plasmid of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope...top requested plasmid of 2018! Additional Resources Quick Guide to All Things Lentivirus 5 Tips for Troubleshooting...Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances...envelope vector pMD2.G This plasmid has been requested nearly 6,000 times (over 800 orders in 2017) and ...the broad usefulness of pMD2.G and VSV-G. This plasmid has been used in studies of the secretome, ubiquitin...ubiquitin ligase, cellular senescence, autophagy, CRISPR, and so much more (find the full, searchable list...

  5. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

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    Blog Post
    ...TRC cloning vector. Lentiviral Plasmids -  If you're familiar with the pLKO plasmids you'll note that those...Actually, these two plasmids from Didier Trono's lab are our two most popular plasmids of the past 10 years...influx of plasmids for reprogramming differentiated cells into pluripotent stem cells. Plasmids from Shinya... have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene ...our stride. We shipped our 100,000th plasmid. A variety of plasmids continued to be popular years after...our collection. CRISPR/Cas9 - At Addgene, 2013 will be known as the "Year of the CRISPR." The newest genome...purposes that we had to create a CRISPR page to keep up with all the new plasmids. So far, we have distributed...

  6. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    ...out our updated CRISPR-Cas resources at www.addgene.org/CRISPR/ to browse CRISPR plasmids, watch informational...information on these new CRISPR screening tools, see our CRISPR/Cas Plasmids: Pooled Libraries webpage...Tet-inducible CRISPR activators and repressor plasmids. (Kearns et al., Development. 2014..) CRISPRs for Xenopus.... 2014..) Our CRISPR-Cas collection of plasmids updates frequently, so visit our CRISPR pages often to... Hot Plasmid list. Other popular plasmids & blog posts @Addgene: The 10 Most Distributed Plasmid Technologies...libraries expand upon the CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA...protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor plasmids from Scot Wolfe's lab. These...

  7. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post
    ...updated CRISPR Guide! Find CRISPR Plasmids for Your Research Browse gRNA Design Tools  ...Central PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene...became much more realistic with the advent of CRISPR. Using CRISPR/Cas9 SAM to "shock and kill" To “shock ...HIV-1 out of the genome with CRISPR/Cas9 Before the introduction of CRISPR, HIV-1 genome editing with ...Treating Muscular Dystrophy with CRISPR Gene Editing Learn to Use CRISPR Pooled Libraries for Genome-Wide... to vanquish these hidden viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel...University has demonstrated two potential strategies for CRISPR-HIV therapeutics - one using dCas9-SAM to activate...

  8. dTAG - You're it!

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    Blog Post
    ...on Addgene.org Browse all CRISPR plasmids CRISPR plasmids for protein tagging Find validated gRNAs ...1). Plasmids: We generated a series of plasmids that are available from Addgene. These plasmids enable...Addgene Blog Easi-CRISPR: Generating Knock-In and Conditional Mouse Models CRISPR 101: Cas9 Nickase Design...which terminus should be tagged, the dTAG lentiviral plasmids can serve as a rapid means of comparing functionality...Detailed protocols describing the use of these plasmids are available here. The Methods section of Nabet...cloning procedures used to construct the deposited plasmids. Molecules: We synthesized dTAG molecules by conjugating...degradation of FKBP12F36V-BRD4 using CRISPR/Cas9-mediated knock-in, and to evaluate in vivo degradation....

  9. X-CHIME: Context Dependent Germline Knockout in Immune Cells

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    Blog Post
    ...Editing CRISPR Cheat Sheat Resources from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions in...strains and lentiviral expression vectors (Table 1). The four systems Name Function Plasmid Transduction...deposit also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the first ... Addgene blog CRISPR101: Validating Your Genome Edit How to Design Your gRNA for CRISPR Genome Editing...time-consuming, as it only changes one gene at a time. CRISPR-based systems enable gene knockout in immune cells...and in combination with different genes. Having CRISPR systems for assessing genes in defined contexts...first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a Postdoctoral Fellow in...

  10. Hot Plasmids Spring 2024

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    Blog Post
    ...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.  ...pooled CRISPR screens By Emily Bentley Building on their CROPseq method for optical pooled CRISPR screening...2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative...Huntington’s Disease, including plasmids, antibodies, viral vector preps, CRISPR tools, and more.  ...Multiplexed perturbation and decoding in pooled CRISPR screens Improved prime editor PE7 Antibodies to...developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi (Walton et al. 2024... over the original CROPseq. Find CROPseq-multi plasmids here Walton, R.T., Qin, Y., Blainey, P.C. (2024...

  11. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post
    ...received over 13,000 plasmids requests for COVID-19 related plasmids. These plasmids have reached labs in...COVID-19: CRISPR-Based Detection Tools. The CRISPR Journal 3:142–145. https://doi.org/10.1089/crispr.2020.0030...small collection of plasmids for coronavirus research, including a handful of plasmids that have been in...some highlights of the plasmids shared over the last year and how these plasmids have been used to accelerate...circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited from... labs also deposited plasmids for producing COVID-19 diagnostic enzymes. Plasmids in this toolkit include...These plasmids contain both untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids that ...

  12. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post
    ...Silvana Konermann, the first generation of CRISPR-Cas9 plasmids were not designed with gene activation in... the CRISPR system was a large one.  The question for her then was this: Can you engineer CRISPR-Cas9 ...Two-way CRISPR screening In October, Jonathan Weissman’s lab introduced additional pooled CRISPR libraries...conducted earlier by the Zhang lab for GeCKO, a lentiviral CRISPR library enabling genome-scale, knockout screening...you review the steps for how to use pooled lentiviral CRISPR libraries and keep the following in mind: ...CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's...lab describe just such a system, which they call CRISPR/Cas9 Synergistic Activation Mediator or SAM. SAM...

  13. Plasmids 101: Screens vs. Selections

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    Blog Post
    ... Addgene Blog CRISPR libraries Genome-wide screens with CRISPR Lentiviral CRISPR libraries ...with small molecules/drugs. With the advent of CRISPR, genome-wide knockout screens are possible with...While the entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically...downstream manipulations as a forward or reverse screen (CRISPR, drug, etc.), just with an added baseline component...component. Alternative screening techniques While CRISPR and drugs dominate the screening scene, there are... worth considering. RNAi screens are similar to CRISPR, but instead of knocking out the genes of interest... be lethal to cells and thus not assayable with CRISPR. Another method, popularized in yeast, are temperature...

  14. Hot Plasmids and Viral Preps - September 2021

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    Blog Post
    ...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources ...s12915-020-00929-7    The CRISPR corner By Jennifer Tsang New CRISPR plasmids are always being added to...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...A new tool for enriching ABE-targeted cells The CRISPR Corner New ready-to-use viral preps   Improved... Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited cell populations...populations without end-point sequencing assays. These plasmids use “Cas9-mediated adenosine transient reporter...

  15. Pooled Library Amplifications

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    Blog Post
    ...recombined plasmid to overtake full-size plasmids (or for smaller plasmids to overtake larger plasmids, if the...How many unique plasmids does your library contain?), the variance of size of plasmids in your library...Amplification Type Link to Protocol CRISPR Knockout Human CRISPR Knockout Pooled Library (Brunello) ...Amplification Protocol (PDF, 88.2 KB) CRISPR Knockout Human CRISPR Knockout Pooled Library (GeCKO v2) ...Protocol (DOCX, 17 KB)   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional... Protocol (DOCX, 15 KB) CRISPR Activation Human Genome-wide CRISPRa-v2 Libraries 104,540–209,080...Re-Amplification Protocol (PDF, 71 KB) CRISPR Activation Human Subpooled CRISPRa-v2 Libraries 12,285–20,145 ...

  16. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ...comparisons of CRISPR parts. The Fragmid system was used to assess how different lentiviral plasmid backbone...more of these kits, as there are many options! CRISPR CRISPR-Cas can harness the power of MoClo to expand...repurposed for CRISPR applications (gene insert = Cas9 + gRNA), Addgene distributes specialized CRISPR kits too... made comparisons between CRISPR components expressed from different plasmid backbones. They noted that...including conditional gene expression, multiplexed CRISPR, and chromosomal integration of larger expression...targetability and effectiveness. Perhaps the most intuitive CRISPR MoClo application is to assemble multiple unique...edit optimization, and more. While there are many CRISPR-Cas libraries and tools available for certain model...

  17. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...Addgene Blog Read Our CRISPR 101 Posts Read Our Plasmids 101 Posts Read All of Plasmid Technology Posts ...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ... control over CRISPR/Cas9 mediated genome modification and gene expression. The plasmids associated with...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase...Nature Methods. 2015 Aug 31. CRISPR-Display: A novel CRISPR technology for targeting RNA cargos...technique, called CRISPR-Display, that may help answer this difficult question. CRISPR-Display uses the...

  18. The AAVantages of AAV in CRISPR Screens

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    Blog Post
    ...Limitation for CRISPR Delivery Genome-wide Screening Using CRISPR Performing In Vivo CRISPR Screens Using...development and improvement of CRISPR-based technologies over the past decade. CRISPR screens are easily customizable...resources at addgene.org CRISPR pooled libraries Guide to pooled libraries CRISPR guide AAV guide ...Most CRISPR screens rely on lentiviral systems, a fact supported by the wide array of lentiviral-based...vivo CRISPR screens. AAV vectors can be made at high titers equal to those achieved with lentivirus but...cargo — in this case, the CRISPR components — are expressed. Unlike lentivirus, cargo delivered by AAV ...lentiviral-based CRISPR libraries available at Addgene. The large packaging capacity and stable expression of lentiviral...

  19. Isolating a Monoclonal Cell Population by Limiting Dilution

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    Protocol
    ...a loading control. 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described... Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature... pool of stable cells. Transducing cells with lentivirus results in a heterogenous polyclonal population...protocol for generating stable cell lines with lentivirus . Day 0: (optional) Seed cells for the generation...protocol for generating stable cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is...expressing Cas9. A549 cells were transduced with lentiCas9-Blast 1 and then selected with 1 μg/mL blasticidin...lines generated from A549 cells transduced with lentiCas9-Blast 1 . A549 cells were transduced (MOI = 37...

  20. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

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    Blog Post
    ...gRNA for CRISPR Genome Editing CRISPR-Cas9: Tips for Optimizing sgRNA Activity Lentiviral CRISPR Libraries...tandem. They went on to show that their CRISPR/Cas9-nuclease and CRISPR/Cas9-nickase vectors work successfully...based on earlier evidence showing that a CRISPR/Cas9 plasmid could be injected directly into mice for ...Find the Mulitplex CRISPR/Cas9 Assembly System Kit at Addgene here. Other Popular CRISPR Posts: How to ...established all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering is now...multiplexity is one of the most advantageous properties of CRISPR/Cas9 compared to ZFNs and TALENs,” said Tetsushi...system offers ease and efficiency for all-in-one CRISPR/Cas9 vectors expressing up to seven guide RNAs ...
Showing: 41 - 60 of 119 results